The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out...The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.展开更多
Cryptosporidium is a protozoan parasite belonging to the phylum Apicomplexa that causes self-limiting diarrhea in immunocompetent individuals, and it may also cause chronic and life-threatening diarrhea in those that ...Cryptosporidium is a protozoan parasite belonging to the phylum Apicomplexa that causes self-limiting diarrhea in immunocompetent individuals, and it may also cause chronic and life-threatening diarrhea in those that are immunocompromised[1]. The two main routes of Cryptosporidium transmission are via water and food. At least 30 Cryptosporidium species have been confirmed, including C. andersoni, with more than 70 genotypes of undefined species.展开更多
Objective Pigs, as hosts of zoonotic Cryptosporidium species/genotypes, are domestic animals with public health significance. The present study was to characterize the infection rate and species/genotype of Cryptospor...Objective Pigs, as hosts of zoonotic Cryptosporidium species/genotypes, are domestic animals with public health significance. The present study was to characterize the infection rate and species/genotype of Cryptosporidium in pre-weaned and post-weaned pigs from Shanghai and Shaoxing, China. Methods A total of 208 fecal samples （42 from pre-weaned piglets, and 166 from post-weaned pigs） were examined by nested PCR of the 185 rRNA gene and analyzed by phylogenetic DNA fragment sequencing of secondary PCR products. Results Infection was detected in 79 samples （19/42 pre-weaned piglets, and 60/166 post-weaned pigs）. C. suis （14/79） and Cryptosporidium pig genotype II （65/79） were identified; piglets were more susceptible to the former （13/14） and post-weaned pigs to the latter （59/65）. Conclusion Infection of Cryptosporidium spp. in pigs was age-specific; piglets were more susceptible to C. suis while pigs were more susceptible to Cryptosporidium pig genotype II. These findings combined with the isolation of the two Cryptosporidium from water suggest that pigs may be a source of zoonotic Cryptosporidium water pollution. Improvements in pig feeding practices, sewage discharge, feces disposal and field worker protection are therefore important to prevent potential public health problems.展开更多
We aimed to assess the risks of Cryptosporidium and Giardia infections associated with drinking water for local residents,based on a quantitative microbial risk assessment,in three densely populated regions of China.I...We aimed to assess the risks of Cryptosporidium and Giardia infections associated with drinking water for local residents,based on a quantitative microbial risk assessment,in three densely populated regions of China.In total,45 source water samples and 45 treated water samples were collected from June to December 2014.展开更多
基金supported by International Collaboration on Drug and Diagnostics Innovation of Tropical Diseases in China (International S&T Cooperation 2010DFB73280)Technology Development Research for Science Research Institute (2011EG150312)the Youth Project of Shanghai Municipal Health Bureau (87)
文摘The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.
基金supported by the Chinese Special Program for Scientific Research of Public Health [No.201502021 to JC]the National Natural Science Foundation of China [No.81371841 and 81772225 to JC]the Fourth Round of Three-Year Public Health Action Plan of Shanghai,China [No.15GWZK0101 to JC]
文摘Cryptosporidium is a protozoan parasite belonging to the phylum Apicomplexa that causes self-limiting diarrhea in immunocompetent individuals, and it may also cause chronic and life-threatening diarrhea in those that are immunocompromised[1]. The two main routes of Cryptosporidium transmission are via water and food. At least 30 Cryptosporidium species have been confirmed, including C. andersoni, with more than 70 genotypes of undefined species.
基金supported by grants from the Chinese Special Program for Scientific Research of Public Health (No.200802012)the National S&T Major Program of China (No.2009ZX10004-201 and No.2012ZX10004-201)
文摘Objective Pigs, as hosts of zoonotic Cryptosporidium species/genotypes, are domestic animals with public health significance. The present study was to characterize the infection rate and species/genotype of Cryptosporidium in pre-weaned and post-weaned pigs from Shanghai and Shaoxing, China. Methods A total of 208 fecal samples （42 from pre-weaned piglets, and 166 from post-weaned pigs） were examined by nested PCR of the 185 rRNA gene and analyzed by phylogenetic DNA fragment sequencing of secondary PCR products. Results Infection was detected in 79 samples （19/42 pre-weaned piglets, and 60/166 post-weaned pigs）. C. suis （14/79） and Cryptosporidium pig genotype II （65/79） were identified; piglets were more susceptible to the former （13/14） and post-weaned pigs to the latter （59/65）. Conclusion Infection of Cryptosporidium spp. in pigs was age-specific; piglets were more susceptible to C. suis while pigs were more susceptible to Cryptosporidium pig genotype II. These findings combined with the isolation of the two Cryptosporidium from water suggest that pigs may be a source of zoonotic Cryptosporidium water pollution. Improvements in pig feeding practices, sewage discharge, feces disposal and field worker protection are therefore important to prevent potential public health problems.
基金supported by the Chinese Special Program for Scientific Research of Public Health[201502021]the Fourth Round of the Three-Year Public Health Action Plan of Shanghai,China[15GWZK0101]。
文摘We aimed to assess the risks of Cryptosporidium and Giardia infections associated with drinking water for local residents,based on a quantitative microbial risk assessment,in three densely populated regions of China.In total,45 source water samples and 45 treated water samples were collected from June to December 2014.
