为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,...为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.展开更多
For the first time we fabricated ZnO membranes with thicknesses of 2.4 nm by a facile one-pot synthesis in aqueous solution.The crystal analysis revealed that the hexagonal ZnO membranes were about 10 atomic layers in...For the first time we fabricated ZnO membranes with thicknesses of 2.4 nm by a facile one-pot synthesis in aqueous solution.The crystal analysis revealed that the hexagonal ZnO membranes were about 10 atomic layers in thickness.The ZnO membranes bent,scrolled,intersected with each other,and self-assembled to particles in micrometre size.The hierarchical assemblies showed sponge-like structures with room inside.In the growth process,a cationic polyelectrolyte was utilized to modulate growth behavior of the ZnO crystals.As a result,the preferred growth direction of ZnO membranes is along 0110,which was perpendicular to[0001]growth direction in a typical hydrothermal synthesis.The growth mechanism of the membranes was also discussed.展开更多
ZnO nanorod arrays were fabricated in aqueous solutions under external voltages.The morphology and length of the nanorods could be readily controlled by varying such parameters as the voltage magnitude,growth time,sol...ZnO nanorod arrays were fabricated in aqueous solutions under external voltages.The morphology and length of the nanorods could be readily controlled by varying such parameters as the voltage magnitude,growth time,solution concentration and substrate type.The external voltage,which made the adsorption of the Zn 2+ cation-containing complexes more possible,modified the growth behavior of the ZnO crystals and played a key role in guaranteeing the orderliness of the arrays.The increase in the nanorod length with the prolonged growth time gradually saturated due to the balance between the growth and the erosion.The ZnO nanorods respectively grown on the Zn and Si substrates differed considerably in both morphology and defect concentration.Field emission was extracted from arrays of nanorods with tapered ends.This field-assisted solution route for fabricating ZnO nanorods featured simplicity in manipulation,inexpensiveness in instrumentation,and effectiveness in controlling the morphology and length.展开更多
文摘为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.
基金supported by the Ministry of Science and Technology of China(Grant Nos.2013CB933604 and 2010CB934203)the National Natural Science Foundation of China(Grant Nos.61171023 and 61076057)
文摘For the first time we fabricated ZnO membranes with thicknesses of 2.4 nm by a facile one-pot synthesis in aqueous solution.The crystal analysis revealed that the hexagonal ZnO membranes were about 10 atomic layers in thickness.The ZnO membranes bent,scrolled,intersected with each other,and self-assembled to particles in micrometre size.The hierarchical assemblies showed sponge-like structures with room inside.In the growth process,a cationic polyelectrolyte was utilized to modulate growth behavior of the ZnO crystals.As a result,the preferred growth direction of ZnO membranes is along 0110,which was perpendicular to[0001]growth direction in a typical hydrothermal synthesis.The growth mechanism of the membranes was also discussed.
基金supported by the National Natural Science Foundation of China (Grant No. 61171023)the National Basic Research Program of China ("973" Project) (Grant No. 2010CB934203)
文摘ZnO nanorod arrays were fabricated in aqueous solutions under external voltages.The morphology and length of the nanorods could be readily controlled by varying such parameters as the voltage magnitude,growth time,solution concentration and substrate type.The external voltage,which made the adsorption of the Zn 2+ cation-containing complexes more possible,modified the growth behavior of the ZnO crystals and played a key role in guaranteeing the orderliness of the arrays.The increase in the nanorod length with the prolonged growth time gradually saturated due to the balance between the growth and the erosion.The ZnO nanorods respectively grown on the Zn and Si substrates differed considerably in both morphology and defect concentration.Field emission was extracted from arrays of nanorods with tapered ends.This field-assisted solution route for fabricating ZnO nanorods featured simplicity in manipulation,inexpensiveness in instrumentation,and effectiveness in controlling the morphology and length.
