Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed ...Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquiturn) demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.展开更多
随着中非合作规模和体量的不断扩大,中非公共卫生合作所蕴含的机遇也在持续上升。疟疾是中非公共卫生合作的主要领域之一,特别是中国消除疟疾之后,如何把中国疟疾防控和消除的经验结合非洲当地疟疾流行形势,加速非洲疟疾的控制和消除,...随着中非合作规模和体量的不断扩大,中非公共卫生合作所蕴含的机遇也在持续上升。疟疾是中非公共卫生合作的主要领域之一,特别是中国消除疟疾之后,如何把中国疟疾防控和消除的经验结合非洲当地疟疾流行形势,加速非洲疟疾的控制和消除,是一个重要的科学问题。本文就中非疟疾防控合作历史、复方青蒿素快速清除疟疾项目、中英坦疟疾控制试点项目、中非消除疟疾机构间合作网络(the institution-based network of China-Africa cooperation on malaria elimination, INCAM)、与世界卫生组织合作助力非洲疟疾防控、国内民间组织在中非疟疾合作中的贡献等进行回顾性研究,分析了中非合作控制和消除疟疾面临的挑战,并对未来的合作方向进行展望。展开更多
基金supported by grants from the Chinese Special Program for Scientific Research of Public Health (200802012)Chinese National Key Program for Infectious Diseases of China (2009ZX10004‐201, and 2008ZX10004‐002)
文摘Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquiturn) demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.
文摘随着中非合作规模和体量的不断扩大,中非公共卫生合作所蕴含的机遇也在持续上升。疟疾是中非公共卫生合作的主要领域之一,特别是中国消除疟疾之后,如何把中国疟疾防控和消除的经验结合非洲当地疟疾流行形势,加速非洲疟疾的控制和消除,是一个重要的科学问题。本文就中非疟疾防控合作历史、复方青蒿素快速清除疟疾项目、中英坦疟疾控制试点项目、中非消除疟疾机构间合作网络(the institution-based network of China-Africa cooperation on malaria elimination, INCAM)、与世界卫生组织合作助力非洲疟疾防控、国内民间组织在中非疟疾合作中的贡献等进行回顾性研究,分析了中非合作控制和消除疟疾面临的挑战,并对未来的合作方向进行展望。