【目的】制备抗罗非鱼湖病毒(Tilapia Lake Virus,TiLV)S10节段基因编码蛋白的单克隆抗体(Monoclonal antibody,MAb)。【方法】构建含有TiLV S10基因节段的重组表达质粒pET32a-S10,并将其转化至BL21(DE3)感受态细胞,经IPTG诱导表达和镍...【目的】制备抗罗非鱼湖病毒(Tilapia Lake Virus,TiLV)S10节段基因编码蛋白的单克隆抗体(Monoclonal antibody,MAb)。【方法】构建含有TiLV S10基因节段的重组表达质粒pET32a-S10,并将其转化至BL21(DE3)感受态细胞,经IPTG诱导表达和镍柱纯化获得高纯度重组S10蛋白;用纯化的重组蛋白免疫6~8周龄雌性BALB/c小鼠多次后,取其脾细胞与SP2/0细胞进行融合获得杂交瘤细胞,采用有限稀释法和ELISA方法,筛选获得2株能稳定分泌抗S10蛋白MAb的杂交瘤细胞株,分别命名为2C3和2E3,并对其特异性、亚型和效价进行分析。【结果】Western blot结果显示,2C3和2E3均能识别S10重组蛋白及TiLV,间接免疫荧光试验(IFA)结果表明,2C3和2E3只与TiLV感染的TiB细胞呈阳性反应。亚型检测结果显示,2C3抗体为IgG1/к型,2E3抗体为IgG2a/к型。抗体效价测定结果表明,两种MAb的效价分别为1∶12800,1∶51200。【结论】抗TiLV-S10蛋白MAb特异性强、效价高,可为后续TiLV疫苗的研发、免疫学方法的建立及S10蛋白功能的研究提供重要材料和支撑。展开更多
The Tianshan orogenic belt is a major part of the southern Central Asian Orogenic Belt（CAOB）,extending from west to east for over 2500 km through Uzbekistan,Tajikistan,Kyrgyzstan and Kazakhstan to Xinjiang in NW Chi...The Tianshan orogenic belt is a major part of the southern Central Asian Orogenic Belt（CAOB）,extending from west to east for over 2500 km through Uzbekistan,Tajikistan,Kyrgyzstan and Kazakhstan to Xinjiang in NW China,and contains the record of multi-phase tectonothermal evolution.Till now.展开更多
Late Silurian-early Devonian mafic--intermediate dykes occur in the Xiemisitai mountain of the northern West Junggar. However, their ages, petrogenesis and geodynamic process remain to be unraveled. We report firstly ...Late Silurian-early Devonian mafic--intermediate dykes occur in the Xiemisitai mountain of the northern West Junggar. However, their ages, petrogenesis and geodynamic process remain to be unraveled. We report firstly in situ zircon U–Pb and hornblende 40Ar-39Ar ages, geochemical and in situ zircon Hf isotopic data for the Xiemisitai dykes. The Xiemisitai dykes give zircon U–Pb ages of 416.7±2.1 Ma and 405.1±2.5 Ma and hornblende 40Ar-39Ar age of 405.9±4.9 Ma, respectively. They are characterized by high εHf(t)values(+11.9 to +15.5), suggesting a depleted mantle features. The Xiemisitai dykes show low Mg#(46–59) and low Cr(11.3–197 ppm) and Ni(19.9–102 ppm) abundances indicating that they have experienced significant fractional crystallization. These dykes contain hornblende and biotite and display negative Nb-Ta-Ti anomalies, enrichment of LREEs, LILEs and depletion of HREEs and HFSEs with high Ba/La ratios, similar with an origin from a depleted lithospheric mantle metasomatized by subducted slabderived fluids. In addition, the Xiemisitai dykes are plotted within melting trends with little to no garnet(Cpx:Grt=6:1) in their source. The La/Yb vs. Tb/Yb plot also indicates the presence of less than 1% residual garnet in the source region for the Xiemisitai dykes. Therefore, it can be inferred that the Xiemisitai dykes were generated at a correspondingly shallow depth, mostly within the spinel stability field. Finally, the Xiemisitai dykes were most probably generated by partial melting of metasomatized lithospheric mantle in relatively shallow level(<80 km). They could be possibly triggered by the asthenospheric upwelling as a result of the rollback of the subducted Irtysh-Zaysan oceanic lithosphere.展开更多
基金supported by the Major Basic Research Project of the Ministry of Science and Technology of China(Grant No.2014CB448000)National Science Foundation of China(Grant Nos..41473053 and 41573045)a grant of Chinese Ministry of Land and Resources(Grant No.201211074–05)
文摘The Tianshan orogenic belt is a major part of the southern Central Asian Orogenic Belt（CAOB）,extending from west to east for over 2500 km through Uzbekistan,Tajikistan,Kyrgyzstan and Kazakhstan to Xinjiang in NW China,and contains the record of multi-phase tectonothermal evolution.Till now.
基金supported by the National Key R&D 445 Program of China(2017YFC0601206)the Strategic Priority Research Program(B)of the Chinese Academy of Sciences(XDB18020203)+1 种基金the Opening Foundation of State Key Laboratory of Continental Dynamics,Northwest University(17LCD04)National Science Foundation of China(Grant nos.41473053,41573045,41611530698,41873060 and 41830216)
文摘Late Silurian-early Devonian mafic--intermediate dykes occur in the Xiemisitai mountain of the northern West Junggar. However, their ages, petrogenesis and geodynamic process remain to be unraveled. We report firstly in situ zircon U–Pb and hornblende 40Ar-39Ar ages, geochemical and in situ zircon Hf isotopic data for the Xiemisitai dykes. The Xiemisitai dykes give zircon U–Pb ages of 416.7±2.1 Ma and 405.1±2.5 Ma and hornblende 40Ar-39Ar age of 405.9±4.9 Ma, respectively. They are characterized by high εHf(t)values(+11.9 to +15.5), suggesting a depleted mantle features. The Xiemisitai dykes show low Mg#(46–59) and low Cr(11.3–197 ppm) and Ni(19.9–102 ppm) abundances indicating that they have experienced significant fractional crystallization. These dykes contain hornblende and biotite and display negative Nb-Ta-Ti anomalies, enrichment of LREEs, LILEs and depletion of HREEs and HFSEs with high Ba/La ratios, similar with an origin from a depleted lithospheric mantle metasomatized by subducted slabderived fluids. In addition, the Xiemisitai dykes are plotted within melting trends with little to no garnet(Cpx:Grt=6:1) in their source. The La/Yb vs. Tb/Yb plot also indicates the presence of less than 1% residual garnet in the source region for the Xiemisitai dykes. Therefore, it can be inferred that the Xiemisitai dykes were generated at a correspondingly shallow depth, mostly within the spinel stability field. Finally, the Xiemisitai dykes were most probably generated by partial melting of metasomatized lithospheric mantle in relatively shallow level(<80 km). They could be possibly triggered by the asthenospheric upwelling as a result of the rollback of the subducted Irtysh-Zaysan oceanic lithosphere.
