Objective Estrogens play an important role in intrinsic skin aging.The associated changes in global gene expression are poorly understood.Methods We used the Illumina microarray platform to obtain comprehensive gene e...Objective Estrogens play an important role in intrinsic skin aging.The associated changes in global gene expression are poorly understood.Methods We used the Illumina microarray platform to obtain comprehensive gene expression profiles in female Chinese Han skin,and confirmed the data by quantitative real-time PCR （Q-RT-PCR）.Results We found 244 genes significantly related to estrogen-associated intrinsic skin aging,and some of these genes were confirmed by Q-RT-PCR.We also performed functional analysis by both Gene Ontology annotation and enrichment of the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways database.The functional analysis revealed 11 biological pathways （including the KEGG pathways,the mitogen-activated protein kinase signaling pathway and metabolic pathways）,that were associated with multiple cellular functions which may be involved in intrinsic skin aging.Conclusion This study suggests that estrogen-associated intrinsic skin aging is a complicated biological process involving many genes and pathways.展开更多
Background Maxillary anterior segmental distraction osteogenesis （MASDO） is a recently used method for correction of severe maxillary retrusion in cleft lip and palate （CLP） patients.In this article,we evaluated t...Background Maxillary anterior segmental distraction osteogenesis （MASDO） is a recently used method for correction of severe maxillary retrusion in cleft lip and palate （CLP） patients.In this article,we evaluated the feasibility of MASDO using rigid external distraction （RED） and rapid orthodontic tooth movement to correct severe maxillary retrusion in CLP patients.Methods Fourteen male and five female complete CLP patients between the ages of 18 and 22 years （mean age 19.7 years） at the time of distraction,with severe maxillary retrusion,were treated with the rigid external distraction （RED） device after maxillary anterior osteotomy.Rapid orthodontic tooth movement was started one week after the MASDO.Standard profile photographic,cephalometric films were obtained preoperatively and after therapy.Sella-nasion-point A （SNA） and Sella-nasion-point B （SNB） angles were measured to reflect changes in maxillary and mandibular position,and the distance between anterior nasal spine and posterior nasal spine （ANS-PNS） was measured to represent the maxillary dental arch length.Results The SNA angle increased from an average of 74.6° （range 73.0°-78.0°）,preoperatively,to 83.4° （range 78.6°-88.0°） after the RED was removed （P ＜0.01）.All cases of severe maxillary retrusion were improved.Nine patients＇ profiles became harmonious after therapy.One patient had a bimaxillary protrusion deformity and needed further surgery.The regenerate alveolar crest and edentulous space on both segments was almost completely eliminated after rapid orthodontic tooth movement.Conclusion MASDO with the RED system and rapid orthodontic tooth movement is a successful way of correcting severe maxillary retrusion in CLP patients.展开更多
文摘Objective Estrogens play an important role in intrinsic skin aging.The associated changes in global gene expression are poorly understood.Methods We used the Illumina microarray platform to obtain comprehensive gene expression profiles in female Chinese Han skin,and confirmed the data by quantitative real-time PCR （Q-RT-PCR）.Results We found 244 genes significantly related to estrogen-associated intrinsic skin aging,and some of these genes were confirmed by Q-RT-PCR.We also performed functional analysis by both Gene Ontology annotation and enrichment of the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways database.The functional analysis revealed 11 biological pathways （including the KEGG pathways,the mitogen-activated protein kinase signaling pathway and metabolic pathways）,that were associated with multiple cellular functions which may be involved in intrinsic skin aging.Conclusion This study suggests that estrogen-associated intrinsic skin aging is a complicated biological process involving many genes and pathways.
文摘Background Maxillary anterior segmental distraction osteogenesis （MASDO） is a recently used method for correction of severe maxillary retrusion in cleft lip and palate （CLP） patients.In this article,we evaluated the feasibility of MASDO using rigid external distraction （RED） and rapid orthodontic tooth movement to correct severe maxillary retrusion in CLP patients.Methods Fourteen male and five female complete CLP patients between the ages of 18 and 22 years （mean age 19.7 years） at the time of distraction,with severe maxillary retrusion,were treated with the rigid external distraction （RED） device after maxillary anterior osteotomy.Rapid orthodontic tooth movement was started one week after the MASDO.Standard profile photographic,cephalometric films were obtained preoperatively and after therapy.Sella-nasion-point A （SNA） and Sella-nasion-point B （SNB） angles were measured to reflect changes in maxillary and mandibular position,and the distance between anterior nasal spine and posterior nasal spine （ANS-PNS） was measured to represent the maxillary dental arch length.Results The SNA angle increased from an average of 74.6° （range 73.0°-78.0°）,preoperatively,to 83.4° （range 78.6°-88.0°） after the RED was removed （P ＜0.01）.All cases of severe maxillary retrusion were improved.Nine patients＇ profiles became harmonious after therapy.One patient had a bimaxillary protrusion deformity and needed further surgery.The regenerate alveolar crest and edentulous space on both segments was almost completely eliminated after rapid orthodontic tooth movement.Conclusion MASDO with the RED system and rapid orthodontic tooth movement is a successful way of correcting severe maxillary retrusion in CLP patients.
