Resistance Evaluation of Soybean Germplasm from Huanghuai Region to Phytophthora Root Rot

The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and Rps1k that soybean cultivars or lines may carry.Virulence formula of 125 Phytophthora sojae isolates were determined using the hypocotyls inoculation technique,the strains,which includ 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars or lines and resistant genes were analyzed using the gene postulating procedure.Eighteen reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars or lines probably carried gene Rps1c and no cultivar may carry genes Rps1a or Rps1k.A few of soybean cultivars or lines from Huanghuai Region carry Rps genes Rps1a,Rps1c and Rps1k and tend to infect by P.sojae,so resistant cultivars or lines need to be bred and popularized actively.

MfSre1基因参与调控桃褐腐病菌对外源渗透的胁迫和对DCFs杀菌剂敏感性

美澳型核果链核盘菌(Monilinia fructicola)引起的褐腐病是严重威胁桃生产的重要真菌性病害。为揭示该病原菌的抗药性机制和发掘新的药剂靶标,本研究利用同源重组分割标记法对MfSre1基因进行了敲除,并研究了该基因的生物学功能。与亲本甾醇14-α-脱甲基化酶抑制剂(DMI)抗性菌株Bmpc7相比,敲除转化子的菌落形态、菌丝生长速率、产孢能力和致病力没有显著变化(P>0.5),对DMIs杀菌剂的敏感性、过氧化氢和高浓度甘油渗透胁迫也没有显著性区别。但对金属离子、糖类、细胞壁/细胞膜破坏剂的敏感性明显降低,对二甲酰亚胺类杀菌剂(DCFs)的抗性水平显著上升。这些结果表明,MfSre1参与调控对部分外源渗透的胁迫和对DCFs杀菌剂的敏感性。