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LIPI-3和LIPI-4共存对致病性单核细胞增生李斯特菌毒力的影响
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作者 钱瑞宣 李楠 +11 位作者 康立超 马勋 刘彩霞 史唯地 寇丽君 任慧杰 祁亚涛 殷中科 刘璐 王静 王正荣 蒋建军 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第5期2027-2036,共10页
【目的】探究单核细胞增生李斯特菌(Listeria monocytogenes,LM)毒力岛3(LIPI-3)和LIPI-4共存对LM毒力的影响,揭示它们对细菌毒力的潜在调节作用,为进一步研究LM的致病机制提供依据。【方法】以LM928(存在LIPI-4)和LM873(同时存在LIPI-3... 【目的】探究单核细胞增生李斯特菌(Listeria monocytogenes,LM)毒力岛3(LIPI-3)和LIPI-4共存对LM毒力的影响,揭示它们对细菌毒力的潜在调节作用,为进一步研究LM的致病机制提供依据。【方法】以LM928(存在LIPI-4)和LM873(同时存在LIPI-3和LIPI-4)2个LM菌株为研究对象,通过侵染HCMEC/D3细胞监测2种菌株对HCMEC/D3细胞的黏附和侵袭情况;通过鸡胚感染试验评估2株菌对鸡胚半数致死量(LD 50)的影响;采用溶血试验来测定菌株的溶血活性;通过流式细胞仪分析HCMEC/D3细胞凋亡情况,以评估菌株诱导细胞凋亡的能力;利用实时荧光定量PCR技术比较2株菌在BHI培养条件下及感染HCMEC/D3细胞后毒力基因的转录水平差异。【结果】LM928和LM873株的黏附率无显著差异(P>0.05),LM928株的侵袭率极显著高于LM873株(P<0.01)。LM873株的LD 50是LM928株的约1000倍;LM928和LM873株的溶血价分别为24和23。LM928株感染24和48 h后诱导的细胞凋亡率显著或极显著高于LM873株(P<0.05;P<0.01),而LM873株的凋亡率与对照组间无显著差异(P>0.05)。LM928和LM873株在BHI培养基中培养时,与LM928株相比,LM873株毒力基因mpl、inlB、inlC、inlP、actA、plcA、plcB和sigB的转录水平均显著或极显著上调(P<0.05;P<0.01),毒力基因hly、inlA和iap的转录水平均极显著或显著下调(P<0.01;P<0.05)。LM928和LM873株侵染HCMEC/D3细胞后,与LM928株相比,LM873株毒力基因inlP、actA、plcA、plcB和prfA的转录水平均显著或极显著上调(P<0.05;P<0.01);毒力基因hly、mpl、inlA、inlB、inlC和iap的转录水平均极显著下调(P<0.01)。【结论】LIPI-3和LIPI-4共存降低了LM的毒力,该结果对进一步研究LIPI-3和LIPI-4在细菌毒力调控中的分子机制和复杂的相互作用具有重要意义。 展开更多
关键词 单核细胞增生李斯特菌(LM) LIPI-3 LIPI-4 毒力 毒力基因
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Signal overcomplete representation and sparse decomposition based on redundant dictionaries 被引量:14
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作者 ZHANG Chunmei yin zhongke +1 位作者 CHEN Xiangdong XIAO Mingxia 《Chinese Science Bulletin》 SCIE EI CAS 2005年第23期2672-2677,共6页
Decomposing a signal based upon redundan dictionaries is a new method for data representation on sig- nal processing. It approximates a signal with an overcom- plete system instead of an orthonormal basis to provide a... Decomposing a signal based upon redundan dictionaries is a new method for data representation on sig- nal processing. It approximates a signal with an overcom- plete system instead of an orthonormal basis to provide a sufficient choice for adaptive sparse decompositions. Re- placing the original data with a sparse approximation can result in not only a higher compression ratio, but also greater flexibility in capturing the inherent structure of the natura signals with the redundancy of dictionaries. This paper gives an overview of a series of recent results in this field, and deals with the relationship between sparsity of signal de- composition and incoherence of dictionaries with BP and MP algorithms. The current and future challenges of the dic- tionary construction are discussed. 展开更多
关键词 信号处理 信号分解 冗余词典 数据表示法
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A fast algorithm for image reconstruction based on sparse decomposition
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作者 yin zhongke WANG Jianying Pierre Vandergheynst 《Frontiers of Electrical and Electronic Engineering in China》 CSCD 2007年第4期432-434,共3页
It is very slow at present to reconstruct an image from its sparse decomposition results.To overcome this one of the main drawbacks in image sparse decomposition,the property of the energy distribution of atoms is stu... It is very slow at present to reconstruct an image from its sparse decomposition results.To overcome this one of the main drawbacks in image sparse decomposition,the property of the energy distribution of atoms is studied in this paper.Based on the property that energy of most atoms is highly concentrated,an algorithm is proposed to fast reconstruct an image from atoms’parameters by limiting atom reconstruction calculating within the atom energy concentrating area.Moreover,methods for fast calculating atom energy and normalization are also put forward.The fast algorithm presented in this paper improves the speed of the image reconstructing by approximately 32 times without degrading the reconstructed image quality. 展开更多
关键词 image processing sparse decomposition matching pursuit image reconstruction
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