Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP

Objective To prepare the PrP specific monoclonal antibodies (mAbs) that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein (HaPrP). Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrPSc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPC from several other mammalian species, including humans and cattles. Immunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPSc. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.

经导管主动脉瓣植入术4例

本文报道了2018至2020年云南省第一人民医院为4例主动脉瓣病变患者行经导管主动脉瓣植入术(transcatheter aortic valve implantation,TAVI),其中3例为主动脉瓣重度狭窄患者,1例为主动脉瓣重度关闭不全患者,重度关闭不全患者采用瓣中瓣方法进行治疗。4例患者手术顺利,术后恢复可,术后10小时开始下床活动。术后5天复查各项指标满意,均已出院。随访至2020年10月,所有患者未见不适。通过4例患者可见,TAVI对外科手术风险评估为中危以上的主动脉瓣狭窄或符合条件的关闭不全的老年患者效果良好,而经股动脉入路是首选的入路方式。