Aim： To investigate the protective effect of diallyl sulfide （DAS）, a constituent of garlic, against testosterone-induced oxidative stress in male Swiss albino mice. Methods： The animals were given low （250 mg/an...Aim： To investigate the protective effect of diallyl sulfide （DAS）, a constituent of garlic, against testosterone-induced oxidative stress in male Swiss albino mice. Methods： The animals were given low （250 mg/animal） and high dose （500 mg/animal） of DAS in corn oil for 7 days along with testosterone （5 mg/kg body weight, i.p.）. At the end of the study period, the prostate and the liver were dissected to determine various antioxidant enzyme levels （catalase, superoxide dismutase, glutathione reductase, glutathione-s-transferase） and lipid peroxidation. Results： In testosterone treated mice, depleted antioxidant enzyme level was accompanied with enhancement in lipid peroxidation in prostate and liver. DAS significantly restored the testosterone-induced antioxidant enzymes and lipid peroxidation in the both organs. These changes appear to be mediated by the antioxidant-enhancing effects of DAS. Conclusion： The results of the present study suggest that DAS is effective in exerting antioxidant effects by inhibiting testosterone-induced oxidative stress and might be helpful in preventing prostate cancer.展开更多
Cancer chemoprevention, a desirable and important facet of biomedical research, Provides a practical approach to identify potentially useful inhibitors of cancer development, and offers an opportunity to study the mec...Cancer chemoprevention, a desirable and important facet of biomedical research, Provides a practical approach to identify potentially useful inhibitors of cancer development, and offers an opportunity to study the mechanism of carcinogenesis. During the recent past a nuInber of compounds have been tested for their anticarcinogenic potential specially constituents of human diet. The enzyme y-glutamyl transpeptidase (CGT), which catalyses the transfer of glutamyl gnups of peptides to other peptides and amino acids, has been proposed as a marker of cell proliferation and neoplasia. It also serves as a tool to evaluate the carcinogenic and cocareinogenic poential of environmental toxicants. In the present investigations, GGT activity induced by carcinogenic polycyclic aromatic hydrocarbons, viz. 7, 12-dimethyl-benz(a) anthracene (DMBA) and benzo(a)Pyrene (BaP was significantly inhibited by diallylsulfide (DAS) and indole-3-carbinol (13C) in mouse skin. DAS and 13C are Constituents of garlic. A significant inhibition in GGT levels was also observed in a strong mitogen (12-o-tetradecanoyl phorbol-13-acelate) induced activity by pretreatment of DAS/13C in mouse skin. Therefore these dielary constituents seem to be strong modifiers of chemically induced carcinogenesis.展开更多
文摘Aim： To investigate the protective effect of diallyl sulfide （DAS）, a constituent of garlic, against testosterone-induced oxidative stress in male Swiss albino mice. Methods： The animals were given low （250 mg/animal） and high dose （500 mg/animal） of DAS in corn oil for 7 days along with testosterone （5 mg/kg body weight, i.p.）. At the end of the study period, the prostate and the liver were dissected to determine various antioxidant enzyme levels （catalase, superoxide dismutase, glutathione reductase, glutathione-s-transferase） and lipid peroxidation. Results： In testosterone treated mice, depleted antioxidant enzyme level was accompanied with enhancement in lipid peroxidation in prostate and liver. DAS significantly restored the testosterone-induced antioxidant enzymes and lipid peroxidation in the both organs. These changes appear to be mediated by the antioxidant-enhancing effects of DAS. Conclusion： The results of the present study suggest that DAS is effective in exerting antioxidant effects by inhibiting testosterone-induced oxidative stress and might be helpful in preventing prostate cancer.
文摘Cancer chemoprevention, a desirable and important facet of biomedical research, Provides a practical approach to identify potentially useful inhibitors of cancer development, and offers an opportunity to study the mechanism of carcinogenesis. During the recent past a nuInber of compounds have been tested for their anticarcinogenic potential specially constituents of human diet. The enzyme y-glutamyl transpeptidase (CGT), which catalyses the transfer of glutamyl gnups of peptides to other peptides and amino acids, has been proposed as a marker of cell proliferation and neoplasia. It also serves as a tool to evaluate the carcinogenic and cocareinogenic poential of environmental toxicants. In the present investigations, GGT activity induced by carcinogenic polycyclic aromatic hydrocarbons, viz. 7, 12-dimethyl-benz(a) anthracene (DMBA) and benzo(a)Pyrene (BaP was significantly inhibited by diallylsulfide (DAS) and indole-3-carbinol (13C) in mouse skin. DAS and 13C are Constituents of garlic. A significant inhibition in GGT levels was also observed in a strong mitogen (12-o-tetradecanoyl phorbol-13-acelate) induced activity by pretreatment of DAS/13C in mouse skin. Therefore these dielary constituents seem to be strong modifiers of chemically induced carcinogenesis.
