Objective Isoliquiritigenin （ISL）, a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigates the mechanisms involved in ISL-induced apoptosis in human cervi...Objective Isoliquiritigenin （ISL）, a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells. Methods Cell viability was evaluated using a 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl-tetrazolium bromide （MTT） assay. Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit. The intracetlular ROS levels were assessed using a 2, 7-dichlorofluorescein probe assay. The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5, 5＇, 6, 6＇-tetra-chloro-1, 1＇, 3, 3＇-tetraethyl-imidacarbocyanine iodide （JC-1）. The degradation of poly-ADP-ribose polymerase （PARP） protein, the phosphorylation of PKR-like ER kinase （PERK）, the phosphorylation of the a-subunit of eukaryotic initiation factor 2 （elF2a）, the expression of the 78 kD glucose-regulated protein （GRP 78）, and the activation of caspase-12 were analyzed via western blot analysis. Results ISL significantly inhibited the proliferation, the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner. Moreover, ISL induced mitochondrial dysfunction, caspase activation, and PARP cleavage, which displayed features of mitochondria dependent on apoptotic signals. Besides, exposure of HeLa cells to ISL triggered endoplasmic reticulum （ER） stress, as indicated by the increase in p-elF2a and GRP78 expression, ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12. Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cel apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways.展开更多
基金supported by the National Natural Science Foundation of China (No. 30960451)Major State Basic Research Development Program (2010CB535003)the Xinjiang production and construction corps funds for distinguished young scientists to ZHENG Qiu Sheng,international cooperation projects (2012BC001)
文摘Objective Isoliquiritigenin （ISL）, a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells. Methods Cell viability was evaluated using a 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl-tetrazolium bromide （MTT） assay. Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit. The intracetlular ROS levels were assessed using a 2, 7-dichlorofluorescein probe assay. The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5, 5＇, 6, 6＇-tetra-chloro-1, 1＇, 3, 3＇-tetraethyl-imidacarbocyanine iodide （JC-1）. The degradation of poly-ADP-ribose polymerase （PARP） protein, the phosphorylation of PKR-like ER kinase （PERK）, the phosphorylation of the a-subunit of eukaryotic initiation factor 2 （elF2a）, the expression of the 78 kD glucose-regulated protein （GRP 78）, and the activation of caspase-12 were analyzed via western blot analysis. Results ISL significantly inhibited the proliferation, the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner. Moreover, ISL induced mitochondrial dysfunction, caspase activation, and PARP cleavage, which displayed features of mitochondria dependent on apoptotic signals. Besides, exposure of HeLa cells to ISL triggered endoplasmic reticulum （ER） stress, as indicated by the increase in p-elF2a and GRP78 expression, ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12. Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cel apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways.
