Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipi...Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipitation, which characterization was detected by TEM, XRD, CMIAS, EDS. MTT assay was used to evaluate the in vitro cytotoxicity test; hemolytic test was carried out to estimate whether it has blood toxicity; Fe2O3 suspended in sterile 0.9% NaCl was intraperitoneally injected into Kumning mouse to calculate the LD50 ; micronucleus （MN） were reckoned to identify whether it is genotoxic. Results：The nanoparticles are brown spherical particles with diameter ranging from 8 to 15 nm, which have good decentralization and stability. The experiments also showed that the toxicity of the material on mouse fibroblast （L-929） cell lines was 0 - 1 degree ; it has no hemolysis activity; LD50 arrived at 5.45 g/kg^-1 after intraperitoneal injection of 1 ml suspension; micronucleus test showed that it has no genotoxic effects either. Conclusion： The results showed that the Fe2O3 nanoparticles are prepared successfully, the self-prepared nanosized Fe2O3 is a kind of high biocompatibility materials and perhaps it is suitable for further application in tumor hyperthermia.展开更多
基金Grant sponsor:National Natural Science Foundation of China,Grant number:30371830Grant sponsor:National Hi-tech research and development program of China,Grant number:2002AA302207+3 种基金 Grant sponsor:Natural Science Foundation of Jiangsu,Grant number:BK2001003Grant sponsor:Hi-tech research pro-gram of Jiangsu,Grant number:BG2001006 Grant sponsor:Key Project of Chinese Traditional Medicine of Jiangsu,Grant number:H027Grant sponsor:Sci-ence Foundation of Southeast University,Grant number:9223001162
文摘Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipitation, which characterization was detected by TEM, XRD, CMIAS, EDS. MTT assay was used to evaluate the in vitro cytotoxicity test; hemolytic test was carried out to estimate whether it has blood toxicity; Fe2O3 suspended in sterile 0.9% NaCl was intraperitoneally injected into Kumning mouse to calculate the LD50 ; micronucleus （MN） were reckoned to identify whether it is genotoxic. Results：The nanoparticles are brown spherical particles with diameter ranging from 8 to 15 nm, which have good decentralization and stability. The experiments also showed that the toxicity of the material on mouse fibroblast （L-929） cell lines was 0 - 1 degree ; it has no hemolysis activity; LD50 arrived at 5.45 g/kg^-1 after intraperitoneal injection of 1 ml suspension; micronucleus test showed that it has no genotoxic effects either. Conclusion： The results showed that the Fe2O3 nanoparticles are prepared successfully, the self-prepared nanosized Fe2O3 is a kind of high biocompatibility materials and perhaps it is suitable for further application in tumor hyperthermia.
