背景与目的:MYD88基因在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中有一定突变率,但其临床病理相关性目前研究报道甚少。该研究旨在分析DLBCL中MYD88基因突变的发生率及与临床病理参数的相关性。方法:收集121例DLBCL...背景与目的:MYD88基因在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中有一定突变率,但其临床病理相关性目前研究报道甚少。该研究旨在分析DLBCL中MYD88基因突变的发生率及与临床病理参数的相关性。方法:收集121例DLBCL患者的临床病理资料,采用免疫组织化学法分析其免疫表型,采用PCR扩增及直接测序法检测MYD88 L265P位点突变情况,采用统计学方法分析MYD88突变与各临床病理参数的相关性。结果:121例DLBCL患者中,38例(31.4%)检测到MYD88 L265P突变。其中男性50例,女性71例,患者性别与该基因突变没有相关性(P=0.609)。年龄≥60岁组MYD88突变率为40.3%(25/62),显著高于<60岁组(13/59,22.0%)(P=0.030)。MYD88突变主要发生在结外部位,其中最常见的是乳腺(12/13,92.3%)、男性生殖系统(10/11,90.9%)、女性生殖系统(5/6,83.3%)及中枢神经系统(4/6,66.7%);结外DLBCL中的MYD88突变率(35/98,35.7%)显著高于结内者(2/20,10%)(P=0.024)。NonGCB型DLBCL中MYD88突变率为39.7%(25/63),显著高于GCB亚型(10/55,18.2%)(P=0.010);结外DLBCL组中MYD88突变与免疫分型的相关性更加显著(P=0.003),而结内组中两者无相关性(P=0.776)。Bcl-2蛋白阳性组(30/77,39.0%)及MYC/Bcl-2蛋白双表达组(19/46,41.3%)中MYD88突变率分别高于Bcl-2阴性组(5/40,12.5%)及非双表达组(16/70,22.9%)(P=0.003和0.034)。Ki-67增殖活性与MYD88基因突变显著相关[高增殖活性组为38.8%(33/85),低增殖活性组为6.3%(2/32)](P<0.001)。该基因突变与MYC蛋白及CD5表达均无相关性(P=0.581和0.759)。结论:MYD88 L265P突变好发于年龄≥60岁、non-GCB起源及特殊结外部位(如乳腺、中枢神经系统及生殖系统等)的DLBCL中,且具有较高增殖指数及MYC/Bcl-2蛋白双表达率;其预后相关性有待积累更多病例进一步分析。MYD88基因突变有望为揭示DLBCL发病机制及靶向治疗提供新的理论依据。展开更多
Based on mine fire fighting practices at the 1110 working face of the Brapukuria Coal Mine,Bangladesh,we introduce and discuss the Y-Inversion Ventilation System,the latest technology used both in mine fire zone manag...Based on mine fire fighting practices at the 1110 working face of the Brapukuria Coal Mine,Bangladesh,we introduce and discuss the Y-Inversion Ventilation System,the latest technology used both in mine fire zone management and the unsealing process.This ventilation system can ensure that all miners breathed fresh air,providing protection for them during fire fighting and unsealing the fire zone.On the other hand,adjusting the amount of air at the working face and forming a CO leakage path controlled the state of the fire and as well ensured that the different fire extinguishing measures could be applied successfully.These are all fundamental techniques which ensured successful fire extinguishing and unsealing of the fire zone.We also analyzed the main reasons for the spontaneous coal combustion that occurred at the 1110 working face.Successful application of advanced composite polymer colloidal perfusion techniques,polymer foam MEA perfusion and fire-prevention technology by infusing nitrogen,used in mine fire zone management and unsealing,are presented.We value the experience with these techniques very highly and are of the opinion that these techniques could be widely used in mine fire fighting practices under similar spontaneous coal combustion conditions elsewhere.展开更多
基金supported by the Key Laboratory Opening Fund of Coalmine Gas & Fire Protection of Henan Province (No.HKLGF200702)
文摘Based on mine fire fighting practices at the 1110 working face of the Brapukuria Coal Mine,Bangladesh,we introduce and discuss the Y-Inversion Ventilation System,the latest technology used both in mine fire zone management and the unsealing process.This ventilation system can ensure that all miners breathed fresh air,providing protection for them during fire fighting and unsealing the fire zone.On the other hand,adjusting the amount of air at the working face and forming a CO leakage path controlled the state of the fire and as well ensured that the different fire extinguishing measures could be applied successfully.These are all fundamental techniques which ensured successful fire extinguishing and unsealing of the fire zone.We also analyzed the main reasons for the spontaneous coal combustion that occurred at the 1110 working face.Successful application of advanced composite polymer colloidal perfusion techniques,polymer foam MEA perfusion and fire-prevention technology by infusing nitrogen,used in mine fire zone management and unsealing,are presented.We value the experience with these techniques very highly and are of the opinion that these techniques could be widely used in mine fire fighting practices under similar spontaneous coal combustion conditions elsewhere.
