将对数生长期的布朗葡萄藻Botryococcus braunii接种到250 m L三角瓶培养体系中,无菌培养液体积为150 m L,分别选择富营养的BG-11培养基、贫营养的f/2培养基及营养水平中等的改良Chu培养基和SE培养基,初始OD690均控制在0.02左右。光暗比...将对数生长期的布朗葡萄藻Botryococcus braunii接种到250 m L三角瓶培养体系中,无菌培养液体积为150 m L,分别选择富营养的BG-11培养基、贫营养的f/2培养基及营养水平中等的改良Chu培养基和SE培养基,初始OD690均控制在0.02左右。光暗比12 h∶12 h、光照强度4 000 lx、(25±2)℃下一次性摇床培养18 d,转速100 r/min,比较了布朗葡萄藻在不同营养供给条件下的生长速度、物质积累和单位产率。结果发现:布朗葡萄藻生长速度取决于培养液的营养元素贫富水平,富营养的BG-11培养基中的比生长速率高于中营养的Chu和SE培养基,而贫营养的f/2培养基中比生长速率最低。但是,富营养的BG-11不利于细胞总脂和总烃的积累,而贫营养f/2培养基更利于布朗葡萄藻细胞积累总脂、总烃和类胡萝卜素。布朗葡萄藻的单位物质产率取决于生长速率和单个细胞物质积累的综合效应;相对而言,贫营养培养基f/2更适合于布朗葡萄藻生产与积累储存性物质(特别是油脂)。所以,富营养的培养基,如BG-11,适合于布朗葡萄藻的快速生长;贫营养的培养基,如f/2,适合于油脂生产与积累;而从营养成分的投入和生产物质的产出效率看,f/2培养基显著优于其他三种培养基。展开更多
Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investi...Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated.The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid.Mono-chromatic Blue-light has advantageous effects on lipid production compared with Red-and White-light.The optimal light spectrum for the protein production was the combination of Red-and Blue-light at a ratio of 4:3 or 5:2.Among the seven fatty acids identified from N.oculata,the contents of C16:0,C18:0,and C18:3(n-3)in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9),C16:2(n-6),and C20:0 were inhibited by Blue-light.Monochromatic Red-and Blue-light and their com-binations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.展开更多
基金supported by the National Natural Sci-ence Foundation of China(Nos.41776156 and 42177459)the Open-End Funds of Jiangsu Key Laboratory of Ma-rine Bioresources and Environment(No.SH20201206).
文摘Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated.The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid.Mono-chromatic Blue-light has advantageous effects on lipid production compared with Red-and White-light.The optimal light spectrum for the protein production was the combination of Red-and Blue-light at a ratio of 4:3 or 5:2.Among the seven fatty acids identified from N.oculata,the contents of C16:0,C18:0,and C18:3(n-3)in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9),C16:2(n-6),and C20:0 were inhibited by Blue-light.Monochromatic Red-and Blue-light and their com-binations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.
