目的探讨绝经后妇女血清视黄醇结合蛋白4(retinol binding protein 4,RBP4)及骨桥蛋白(osteopontin,OPN)水平与骨密度(bone mineral density,BMD)的关系,分析其在绝经后骨质疏松症(postmenopousal osteoporosis,PMOP)中的应用价值。方法...目的探讨绝经后妇女血清视黄醇结合蛋白4(retinol binding protein 4,RBP4)及骨桥蛋白(osteopontin,OPN)水平与骨密度(bone mineral density,BMD)的关系,分析其在绝经后骨质疏松症(postmenopousal osteoporosis,PMOP)中的应用价值。方法对262例绝经后妇女进行研究双能X线骨密度仪测量腰椎1-4(L1-4)和左侧股骨颈(FN),测定血中RBP4,OPN,I型原胶原N-端前肽(PINP),β-胶原降解产物(β-CTX),甲状旁腺素(PTH),骨钙素(OC),血钙(Ca)及血磷(P)水平。ROC曲线分析RBP4及OPN诊断PMOP的价值。结果骨质疏松组血清RBP4(μg/ml)(25.13±4.74, 18.46±4.25和15.30±3.87)及OPN(ng/ml)(13.58±4.42, 10.47±3.46和8.24±3.13)水平均明显高于骨量减少组和正常组,差异有统计学意义(均P <0.05)。相关分析显示,骨质疏松症组血清RBP4及OPN水平与β-CTX呈正相关(r=0.291,0.284,均P <0.05),与Ca,L1-4及左FN骨密度呈负相关(r=-0.247,-0.513,-0.486,-0.265,-0.556,-0.574,均P <0.05)。ROC曲线显示,血清RBP4及OPN水平为21.53μg/ml和12.27 ng/ml是诊断PMOP的最佳截值。结论血清RBP4及OPN水平升高与低BMD相关,是影响PMOP发生的关键因子。展开更多
The antidepressant trimipramine（Tri） enantiomers were successfully separated by capillary electrophoresis（CE） coupled with electrochemiluminescence（ECL） detection in aqueous-organic media. A dual cyclodextrin（C...The antidepressant trimipramine（Tri） enantiomers were successfully separated by capillary electrophoresis（CE） coupled with electrochemiluminescence（ECL） detection in aqueous-organic media. A dual cyclodextrin（CD） system combining β-CD and hydroxypropyl-β-cyclodextrin（HP-β-CD） was used as chiral selector. Acetonitrile（ACN） was added to the running buffer to improve the separation efficiency, detection sensitivity and repeatability. The method was also successfully applied to the chiral separation of Tri in spiked human urine sample.展开更多
基金Supported by the National Natural Science Foundation of China(No20875085)the Fund of Chinese Academy of Sciences(NoKJCX2-YW-H11)
文摘The antidepressant trimipramine（Tri） enantiomers were successfully separated by capillary electrophoresis（CE） coupled with electrochemiluminescence（ECL） detection in aqueous-organic media. A dual cyclodextrin（CD） system combining β-CD and hydroxypropyl-β-cyclodextrin（HP-β-CD） was used as chiral selector. Acetonitrile（ACN） was added to the running buffer to improve the separation efficiency, detection sensitivity and repeatability. The method was also successfully applied to the chiral separation of Tri in spiked human urine sample.
