Hollow multishelled structure(HoMS),a promising and complex multifunctional structural system,features at least two shells that are separated by internal voids.The unique structure endows it with numerous advantages i...Hollow multishelled structure(HoMS),a promising and complex multifunctional structural system,features at least two shells that are separated by internal voids.The unique structure endows it with numerous advantages including low density,high loading capacity,large specific surface area,facilitated mass transport,and multiple spatial confinement effect.In the past twenty years,benefiting from the booming development of synthesis methods,various HoMS materials have been prepared and show promising applications in diverse areas.展开更多
Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensit...Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.21931012,21821005,92163209,52272097,52202354,22293043,52261160573,52072369,and 52301296)the Zhongke-Yuneng Joint R&D Center Program,China(No.ZKYN2022008).
文摘Hollow multishelled structure(HoMS),a promising and complex multifunctional structural system,features at least two shells that are separated by internal voids.The unique structure endows it with numerous advantages including low density,high loading capacity,large specific surface area,facilitated mass transport,and multiple spatial confinement effect.In the past twenty years,benefiting from the booming development of synthesis methods,various HoMS materials have been prepared and show promising applications in diverse areas.
基金supported by the National Basic Research Program of Chi-na (2007CB714506)the National Natural Science Foundation of China (20735005 & 20925517)+1 种基金Science and Technology Committee of Shanghai (10XD1406000 & 09JC1402600)Shanghai Leading Academic Disci-pline B109
文摘Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.