为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。...为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。展开更多
FeP/FeO was prepared on carbon cloth(CC)via hydrothermal method,heat treatment in air,and phosphorization in argon.FeP/FeO/CC presents a porous and loose morphology which is conducive to the exposure of active sites a...FeP/FeO was prepared on carbon cloth(CC)via hydrothermal method,heat treatment in air,and phosphorization in argon.FeP/FeO/CC presents a porous and loose morphology which is conducive to the exposure of active sites and the transfer of reactants.FeP/FeO/CC requires the low overpotentials of 257 and 117 mV(vs.reversible hydrogen electrode(RHE))to achieve the current density of 10 mA_·cm^(-2)for oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)in alkaline KOH solution,respectively.The small Tafel slope values of 36.1 mV_·dec^(-1)(for OER)and 96.2 mV_·dec^(-1)(for HER)indicate that FeP/FeO/CC exhibits the fast electrocatalytic reactive kinetics for OER and HER.In particular,the reaction kinetics of FeP/FeO/CC accelerated with the progress of HER.The charge-transfer resistance(R_(ct))of FeP/FeO/CC is only 11Ω.Excellent bifunctional electrocatalytic performances of FeP/FeO/CC should be attributed to the porous morphology and the lower charge-transfer resistance.展开更多
基金Funded by the Natural Science Foundation of Anhui Higher Education Institution of China(No.2023AH040160)the Natural Science Foundation of Anhui Province(No.1808085QE126)+1 种基金the Hefei Normal University High level Talent Research Startup Fund Project(No.2022rcjj55)the Collaborative Innovation Project of Colleges and Universities in Anhui Province(No.GXXT-2021-091)。
文摘FeP/FeO was prepared on carbon cloth(CC)via hydrothermal method,heat treatment in air,and phosphorization in argon.FeP/FeO/CC presents a porous and loose morphology which is conducive to the exposure of active sites and the transfer of reactants.FeP/FeO/CC requires the low overpotentials of 257 and 117 mV(vs.reversible hydrogen electrode(RHE))to achieve the current density of 10 mA_·cm^(-2)for oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)in alkaline KOH solution,respectively.The small Tafel slope values of 36.1 mV_·dec^(-1)(for OER)and 96.2 mV_·dec^(-1)(for HER)indicate that FeP/FeO/CC exhibits the fast electrocatalytic reactive kinetics for OER and HER.In particular,the reaction kinetics of FeP/FeO/CC accelerated with the progress of HER.The charge-transfer resistance(R_(ct))of FeP/FeO/CC is only 11Ω.Excellent bifunctional electrocatalytic performances of FeP/FeO/CC should be attributed to the porous morphology and the lower charge-transfer resistance.
