目的:制备量子点与葡萄球菌蛋白A(Staphylococcal protein A,SPA)的偶联物,探究偶联反应的最佳条件。方法:采用“经典注入法”制备碲化镉量子点,使用1-乙基-3-(3-二甲氨基丙基)碳二亚胺盐酸盐(EDC)和N-羟基丁二酰亚胺(NHS)作为偶联剂用...目的:制备量子点与葡萄球菌蛋白A(Staphylococcal protein A,SPA)的偶联物,探究偶联反应的最佳条件。方法:采用“经典注入法”制备碲化镉量子点,使用1-乙基-3-(3-二甲氨基丙基)碳二亚胺盐酸盐(EDC)和N-羟基丁二酰亚胺(NHS)作为偶联剂用于偶联量子点和SPA,采用单因素考察法,分别探究EDC、NHS和SPA用量以及反应时间对偶联效率的影响。结果:制备得到不同量子点、EDC、NHS、SPA比例以及不同反应时间的偶联物,其中量子点和EDC的比例为1∶1500,EDC和NHS的比例为1∶2,反应时间为2 h时偶联物荧光强度最强。结论:碲化镉量子点∶EDC∶NHS为1∶1500∶3000,反应时间为2 h时,可以制备出荧光强度较好的量子点SPA偶联物。展开更多
This paper presents 2D wave-current interaction model for evaluating nearly horizontal wave-induced currents in the surf-zone and coastal waters.The hydrodynamic model is the two-dimensional depth-averaged nonlinear s...This paper presents 2D wave-current interaction model for evaluating nearly horizontal wave-induced currents in the surf-zone and coastal waters.The hydrodynamic model is the two-dimensional depth-averaged nonlinear shallow water equations by using an unstructured non-staggered and multiple-level quadtree rectangular mesh,this mesh information is stored in simple data structures and it is easy to obtain a locally high resolution for important region.The intercell fluxes are computed based on the HLL(Harten-Lax-van Leer) approximate Riemann solver with shock capturing capability for computing the dry-to-wet interface of coastal line.The effects of pressure and gravity are included in source term in the model,this treatment can simplify the computation and eliminate numerical imbalance between source and flux terms.The wave model readily provides the radiation stresses that represent the shortwave-averaged forces in a water column for the hydrodynamic model and the wave model takes into account the effect of wave-induced nearshore currents and water level.The coupling model is applied to verify different experimental cases and real life case of considering the wave-current interaction.The calculated results agree with analytical solution,experimental and field data.The results show that the modeling approach presented herein should be useful in simulating the nearshore processes in complicated natural coastal domains.展开更多
文摘目的:制备量子点与葡萄球菌蛋白A(Staphylococcal protein A,SPA)的偶联物,探究偶联反应的最佳条件。方法:采用“经典注入法”制备碲化镉量子点,使用1-乙基-3-(3-二甲氨基丙基)碳二亚胺盐酸盐(EDC)和N-羟基丁二酰亚胺(NHS)作为偶联剂用于偶联量子点和SPA,采用单因素考察法,分别探究EDC、NHS和SPA用量以及反应时间对偶联效率的影响。结果:制备得到不同量子点、EDC、NHS、SPA比例以及不同反应时间的偶联物,其中量子点和EDC的比例为1∶1500,EDC和NHS的比例为1∶2,反应时间为2 h时偶联物荧光强度最强。结论:碲化镉量子点∶EDC∶NHS为1∶1500∶3000,反应时间为2 h时,可以制备出荧光强度较好的量子点SPA偶联物。
基金supported by the National Natural Science Foundation of China (Grant No. 50839001)the research grant from Southeast Regional Research Initiative (SERRI,80037)the Coastal Inlets Research Program,ERDC,US Army Corps of Engineers,Vicksburg,MS,USA
文摘This paper presents 2D wave-current interaction model for evaluating nearly horizontal wave-induced currents in the surf-zone and coastal waters.The hydrodynamic model is the two-dimensional depth-averaged nonlinear shallow water equations by using an unstructured non-staggered and multiple-level quadtree rectangular mesh,this mesh information is stored in simple data structures and it is easy to obtain a locally high resolution for important region.The intercell fluxes are computed based on the HLL(Harten-Lax-van Leer) approximate Riemann solver with shock capturing capability for computing the dry-to-wet interface of coastal line.The effects of pressure and gravity are included in source term in the model,this treatment can simplify the computation and eliminate numerical imbalance between source and flux terms.The wave model readily provides the radiation stresses that represent the shortwave-averaged forces in a water column for the hydrodynamic model and the wave model takes into account the effect of wave-induced nearshore currents and water level.The coupling model is applied to verify different experimental cases and real life case of considering the wave-current interaction.The calculated results agree with analytical solution,experimental and field data.The results show that the modeling approach presented herein should be useful in simulating the nearshore processes in complicated natural coastal domains.
