Study on the mechanism of action of Lizhong Tang in ameliorating non-alcoholic fatty liver disease by regulating mitochondrial autophagy

Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index.Liver pathology was observed,while serum transaminase and blood lipid levels were measured.The protein expression levels of PINK1,Parkin,and LC-3II in rat liver were detected using Western Blot analysis.Results:Compared with the control group,the NAFLD rats exhibited a significant increase in body weight,liver wet weight,liver index,transaminase levels,and blood lipid levels.The expression levels of PINK1,Parkin,and LC3-II protein were significantly decreased(P<0.01).Following intervention with Lizhong Tang,rats in each herbal treatment group displayed a decrease in body weight,liver wet weight,liver index,se-rum transaminase,and blood lipid levels.The expression levels of PINK1,Parkin,and LC-3II rebounded(P<0.05),with the high-dose group demonstrating the most pronounced effects(P<0.01).Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure,swollen hepatocytes,disordered arrangement,and a multi-tude of varying-sized lipid vacuoles within the cytoplasm.Conversely,rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology,with the high-dose group showing the most notable enhancement.Conclusion:Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.

应用黄芪注射液预防子宫肌瘤术后腹部切口脂肪液化的研究

目的探究黄芪注射液在预防子宫肌瘤术后腹部切口脂肪液化的价值。方法选取2016年6月至2017年3月于鄂东医疗集团黄石市妇幼保健院妇产科行开腹手术的34例子宫肌瘤患者作为研究对象进行前瞻性研究分析,随机分成两组后,对照组采用碘伏常规处理术后伤口,研究组加用黄芪注射液外敷伤口。Logistic多因素回归分析可能影响切口脂肪液化的因素,包括年龄、体重指数及外敷药物。培养人脐静脉内皮细胞,不同浓度(0、25、50、100 mg/L)黄芪注射液处理细胞,检测细胞增殖及血管内皮细胞生长因子A的mRNA表达。结果研究组的切口脂肪液化发生率显著降低; Logistic多因素回归分析显示年龄及体重指数对切口脂肪液化的发生无显著影响,而黄芪使用则有显著影响(r=0. 456,P=0. 0156);黄芪注射液可显著促进人脐静脉内皮细胞的增殖(P <0. 001)及血管内皮细胞生长因子A mRNA的表达(P <0. 001)。结论术后切口护理中加用黄芪注射液外用后可显著减少切口脂肪液化的发生率,可能与黄芪能够促进血管内皮细胞增殖及血管内皮细胞生长因子A的表达有关。

麻黄升麻汤对肝硬化腹水并感染大鼠血清炎性因子的干预作用

目的:研究麻黄升麻汤对肝硬化腹水并感染大鼠血清炎性因子的干预作用,并从PI3K/Akt1信号转导通路探讨其作用机制。方法:将80只SD成年健康大鼠随机分为空白组,模型组,溶媒对照组,低、中、高剂量组。采用四氯化碳联合乙醇、高脂饮食法建立肝硬化腹水大鼠模型。全自动生化仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清白蛋白(ALB);显微镜下计数法检测腹水多形核白细胞(PMN);ELISA法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)水平;Western blot法检测肝组织中PI3K p85、p-Akt1蛋白表达量。结果:经干预治疗后,与模型组、溶媒对照组比较,高剂量组、中剂量组ALT、AST、TNF-α、IL-6、IL-8、PMN及肝组织中PI3K p85、p-Akt1蛋白表达量显著降低(P<0.05),且高剂量组较中、低剂量组下降更显著(P<0.05)。结论:麻黄升麻汤可明显改善肝硬化腹水并感染大鼠肝功能,降低血清炎症细胞因子水平,抑制局部炎性反应,其作用机制可能与抑制PI3K/Akt1信号通路的激活有关。