[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the p...[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the positive and negative influences of meteorological condition in late growth stage of wheat (May) on wheat yield in Anyang City were analyzed by using agricultural climatic statistical method. Moreover, the reason and defense measure of green-dry hazard in late growth stage of wheat in the city were studied. [Result] When the sunshine percentage in May > 55%, and rainfall < 45 mm, the wheat yield generally increased. But when it was overcast and rainy, and the sunshine was less, especially monthly rainfall > 80 mm, and monthly sunshine percentage < 55%, the wheat yield generally reduced. The overcast and rainy weather, flood in late growth stage of wheat were easy to cause green-dry yield reduction. The rainless weather even drought weren’t obviously unfavorable for good harvest of wheat. In May, when precipitation was too more, or duration was too long, and the air humidity was too big, the normal water supply and inorganic nutrient transmission were affected. Meanwhile, when the overcast and rainy weather was longer, the sunshine was shorter, and the sunshine intensity weakened in late stage, it wasn’t favorable for accumulation of photosynthetic product, and the normal implementing of grouting process was affected. The measures should be used to prevent and control green-dry yield reduction of wheat, such as discharging water and preventing flood, breeding good seed, scientific planting and reasonable irrigation. [Conclusion] The research provided scientific basis for studying variety improvement, scientific plantation, reasonable irrigation, good quality and high yield of wheat.展开更多
Background Our previous studies demonstrated that mutant IKBα （IKBαM） inhibited the occurrence, growth and angiogenesis of human glioblastoma multiform （GBM）. However, the specific mechanism by which IKBaM regul...Background Our previous studies demonstrated that mutant IKBα （IKBαM） inhibited the occurrence, growth and angiogenesis of human glioblastoma multiform （GBM）. However, the specific mechanism by which IKBaM regulates protein-degrading enzymes secreted from GBM to inhibit invasion and metastasis has remained unclear. The aim of the present study was to investigate the regulatory role and significance of IKBαM genes in the expression of tissue inhibitor of metalloproteinase （TIMP）-2 and matrix metalloproteinase （MMP）-9 in human GBM. Methods We established the following four GBM cell lines stably expressing IKBaM by plasmid construction, gene transfection and screening for IKBαM protein expression： mutant IKBa-transfected cells （G36A-M）, wild-type IKBa-transfected cells （G36A-W）, empty plasmid transfected cells （G36A-P） and untransfected cells （G36A）. The TIMP-2 and MMP-9 expression was detected by RT-PCR and Western blotting. Tumor cells were then implanted subcutaneously into nude mice to establish an animal model of ectopic tumor growth, and TIMP-2 and MMP-9 expression was determined by immunohistochemical methods. Results The results showed that there was a significant increase in TIMP-2 expression and a significant decrease in MMP-9 expression in the G36A-M group at both the RNA and protein levels compared with the G36A-W group, G36A-P group and G36A group. Similar results were observed in the immunohistochemical staining analysis of tumor tissues. In the G36A-M group, TIMP-2 expression was significantly higher while MMP-9 expression was significantly lower than in the other three groups. Conclusions Our findings indicate that IKBaM inhibits the activation of NF-KB. It significantly up-regulates TIMP-2 expression in human malignant glioma cells and down-regulates the expression of MMP-9. Thus, IKBαM maintains the integrity of the extracellular matrix and further inhibits the growth and metastasis of tumor tissues. Chin Med J 2009; 122（2）：205-211展开更多
文摘[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the positive and negative influences of meteorological condition in late growth stage of wheat (May) on wheat yield in Anyang City were analyzed by using agricultural climatic statistical method. Moreover, the reason and defense measure of green-dry hazard in late growth stage of wheat in the city were studied. [Result] When the sunshine percentage in May > 55%, and rainfall < 45 mm, the wheat yield generally increased. But when it was overcast and rainy, and the sunshine was less, especially monthly rainfall > 80 mm, and monthly sunshine percentage < 55%, the wheat yield generally reduced. The overcast and rainy weather, flood in late growth stage of wheat were easy to cause green-dry yield reduction. The rainless weather even drought weren’t obviously unfavorable for good harvest of wheat. In May, when precipitation was too more, or duration was too long, and the air humidity was too big, the normal water supply and inorganic nutrient transmission were affected. Meanwhile, when the overcast and rainy weather was longer, the sunshine was shorter, and the sunshine intensity weakened in late stage, it wasn’t favorable for accumulation of photosynthetic product, and the normal implementing of grouting process was affected. The measures should be used to prevent and control green-dry yield reduction of wheat, such as discharging water and preventing flood, breeding good seed, scientific planting and reasonable irrigation. [Conclusion] The research provided scientific basis for studying variety improvement, scientific plantation, reasonable irrigation, good quality and high yield of wheat.
文摘Background Our previous studies demonstrated that mutant IKBα （IKBαM） inhibited the occurrence, growth and angiogenesis of human glioblastoma multiform （GBM）. However, the specific mechanism by which IKBaM regulates protein-degrading enzymes secreted from GBM to inhibit invasion and metastasis has remained unclear. The aim of the present study was to investigate the regulatory role and significance of IKBαM genes in the expression of tissue inhibitor of metalloproteinase （TIMP）-2 and matrix metalloproteinase （MMP）-9 in human GBM. Methods We established the following four GBM cell lines stably expressing IKBaM by plasmid construction, gene transfection and screening for IKBαM protein expression： mutant IKBa-transfected cells （G36A-M）, wild-type IKBa-transfected cells （G36A-W）, empty plasmid transfected cells （G36A-P） and untransfected cells （G36A）. The TIMP-2 and MMP-9 expression was detected by RT-PCR and Western blotting. Tumor cells were then implanted subcutaneously into nude mice to establish an animal model of ectopic tumor growth, and TIMP-2 and MMP-9 expression was determined by immunohistochemical methods. Results The results showed that there was a significant increase in TIMP-2 expression and a significant decrease in MMP-9 expression in the G36A-M group at both the RNA and protein levels compared with the G36A-W group, G36A-P group and G36A group. Similar results were observed in the immunohistochemical staining analysis of tumor tissues. In the G36A-M group, TIMP-2 expression was significantly higher while MMP-9 expression was significantly lower than in the other three groups. Conclusions Our findings indicate that IKBaM inhibits the activation of NF-KB. It significantly up-regulates TIMP-2 expression in human malignant glioma cells and down-regulates the expression of MMP-9. Thus, IKBαM maintains the integrity of the extracellular matrix and further inhibits the growth and metastasis of tumor tissues. Chin Med J 2009; 122（2）：205-211
