仓储生态因子和烟叶化学成分的改变直接影响烟叶微生物群落结构和功能。以储存在贵阳库(GY)、坛厂库(TC)及紫云库(ZY)的云南保山C3F烟叶为研究对象,对不同陈化时间(0、6、12、18、24个月)的烟叶样品提取微生物总DNA,利用Illumina Hi Se...仓储生态因子和烟叶化学成分的改变直接影响烟叶微生物群落结构和功能。以储存在贵阳库(GY)、坛厂库(TC)及紫云库(ZY)的云南保山C3F烟叶为研究对象,对不同陈化时间(0、6、12、18、24个月)的烟叶样品提取微生物总DNA,利用Illumina Hi Seq平台对细菌的16S rRNA V4区进行高通量测序,并结合主要化学成分分析,以期揭示烟叶陈化过程中细菌群落演替规律及其与烟叶化学成分间的作用关系。研究结果表明,烟叶细菌群落以假单胞菌属、鞘氨醇单胞菌属、寡养单胞菌属、芽孢杆菌属为优势属;随陈化时间的增加,细菌优势群落呈现出变形菌门和厚壁菌门间消长变化的趋势,陈化后期芽孢杆菌(厚壁菌门)优势度明显增强;烟叶陈化过程中,细菌优势功能群变化与化学成分逐级降解间呈显著的相关关系,主要表现为由降解糖类菌群向降解淀粉类菌群,再向降解纤维素类菌群变化的趋势;其中,影响菌群演替的关键因素是水溶性总糖和纤维素。研究结果揭示了在烟叶陈化过程中存在显著的细菌群落演替特征,加深了对烟叶陈化机制的理解,为微生物调控烟叶陈化过程提供了科学依据。展开更多
Forest can be taken as a natural therapy to alleviate perceived anxiety of visitors. Given the geographical difference between urban and rural forest environments, little is known about the urban forest therapy effect...Forest can be taken as a natural therapy to alleviate perceived anxiety of visitors. Given the geographical difference between urban and rural forest environments, little is known about the urban forest therapy effect of anxiety alleviation with reference to the rural forest. In this study, forty-three university students(aged from 19 to 23) were recruited as participants to visit the forest parks at urban and rural areas of Guiyang City on 21 and 23 December 2016. The forest experience was separated by four sceneries. Participants were asked to complete questionnaires by self-evaluating specific anxiety change from 12 questions with scores from 1 to 10 at both entrance and exit of the parks. The Wilcoxon signed-rank test and paired t-test were used to compare the change of anti-anxiety scores during forest bathing and between urban and rural forests, respectively. Results revealed that forest bathing in the urban park can alleviate the anxiety from financial state(P = 0.0028), exam-pass pressure(P = 0.0040) and love-affair relationship(P = 0.0286). Although rural forest bathing can also alleviate the anxiety from financial state(P = 0.0222), meanwhile, it maintained the anxiety about campus life(P < 0.0001). Forest tree richness tended to be higher in the rural forest park than in the urban one, which in contrast decreased the anxiety alleviation from inter-communication in the rural forest park(P = 0.0487). Principle component analysis indicated that participants tended to perceive more decline of anxiety from social contact in the urban forest. In conclusion, university students were recommended to pay a short visit to the urban forest with partners if they felt anxious about personal affairs and felt necessary to talk with others. For general people's visiting, urban forest trees can be controlled in diversity to some extent to look orderly and alleviate perceived anxiety.展开更多
文摘仓储生态因子和烟叶化学成分的改变直接影响烟叶微生物群落结构和功能。以储存在贵阳库(GY)、坛厂库(TC)及紫云库(ZY)的云南保山C3F烟叶为研究对象,对不同陈化时间(0、6、12、18、24个月)的烟叶样品提取微生物总DNA,利用Illumina Hi Seq平台对细菌的16S rRNA V4区进行高通量测序,并结合主要化学成分分析,以期揭示烟叶陈化过程中细菌群落演替规律及其与烟叶化学成分间的作用关系。研究结果表明,烟叶细菌群落以假单胞菌属、鞘氨醇单胞菌属、寡养单胞菌属、芽孢杆菌属为优势属;随陈化时间的增加,细菌优势群落呈现出变形菌门和厚壁菌门间消长变化的趋势,陈化后期芽孢杆菌(厚壁菌门)优势度明显增强;烟叶陈化过程中,细菌优势功能群变化与化学成分逐级降解间呈显著的相关关系,主要表现为由降解糖类菌群向降解淀粉类菌群,再向降解纤维素类菌群变化的趋势;其中,影响菌群演替的关键因素是水溶性总糖和纤维素。研究结果揭示了在烟叶陈化过程中存在显著的细菌群落演替特征,加深了对烟叶陈化机制的理解,为微生物调控烟叶陈化过程提供了科学依据。
基金National Natural Science Foundation of China(No.31600496)National Key Research and Development Program of China(No.2016YFC0500300)Construction Program of Biology First-class Discipline in Guizhou(No.GNYL[2017]009)
文摘Forest can be taken as a natural therapy to alleviate perceived anxiety of visitors. Given the geographical difference between urban and rural forest environments, little is known about the urban forest therapy effect of anxiety alleviation with reference to the rural forest. In this study, forty-three university students(aged from 19 to 23) were recruited as participants to visit the forest parks at urban and rural areas of Guiyang City on 21 and 23 December 2016. The forest experience was separated by four sceneries. Participants were asked to complete questionnaires by self-evaluating specific anxiety change from 12 questions with scores from 1 to 10 at both entrance and exit of the parks. The Wilcoxon signed-rank test and paired t-test were used to compare the change of anti-anxiety scores during forest bathing and between urban and rural forests, respectively. Results revealed that forest bathing in the urban park can alleviate the anxiety from financial state(P = 0.0028), exam-pass pressure(P = 0.0040) and love-affair relationship(P = 0.0286). Although rural forest bathing can also alleviate the anxiety from financial state(P = 0.0222), meanwhile, it maintained the anxiety about campus life(P < 0.0001). Forest tree richness tended to be higher in the rural forest park than in the urban one, which in contrast decreased the anxiety alleviation from inter-communication in the rural forest park(P = 0.0487). Principle component analysis indicated that participants tended to perceive more decline of anxiety from social contact in the urban forest. In conclusion, university students were recommended to pay a short visit to the urban forest with partners if they felt anxious about personal affairs and felt necessary to talk with others. For general people's visiting, urban forest trees can be controlled in diversity to some extent to look orderly and alleviate perceived anxiety.
