1案例1.1简要案情及病史摘要某日,某孕妇因“停经39+3周,见红1 d”入院,当晚行剖宫产术娩出一足月男婴,呼吸道中清理出较多污染羊水,在继续清理呼吸道过程中出现呼吸渐弱、面色发绀,1 min Apgar评分8分。后出现继发性呼吸暂停、心率减慢...1案例1.1简要案情及病史摘要某日,某孕妇因“停经39+3周,见红1 d”入院,当晚行剖宫产术娩出一足月男婴,呼吸道中清理出较多污染羊水,在继续清理呼吸道过程中出现呼吸渐弱、面色发绀,1 min Apgar评分8分。后出现继发性呼吸暂停、心率减慢,抢救约40 min后未见好转,转上级医院进一步救治。进入上级医院重症监护室,患儿面色及周身青紫,胸廓无明显隆起,呼吸急促,吸气三凹征阳性,双肺呼吸音粗,可闻及干性啰音。展开更多
Objective To research the relation between the time-dependent appearances of myofibroblasts during the repair of contused skeletal muscle in rat and wound age determination. Methods A total of 35 SD male rats were div...Objective To research the relation between the time-dependent appearances of myofibroblasts during the repair of contused skeletal muscle in rat and wound age determination. Methods A total of 35 SD male rats were divided into the control and six injured groups according to wound age as follows: 12 h, 1 d, 5 d, 7 d, 10 d and 14 d after injury. The appearances of myofibroblasts were detected by HE staining, immunohistochemistry and confocal laser scanning microscopy. Masson's trichrome staining was utilized to examine collagen accumulation in the contused areas. Results Immunohistochemical staining showed that α-SMA+myofibroblasts were initially observed at 5 d post-injury. The average ratio of myofibroblasts was highest at 14 d post-injury, with all samples, ratios more than 50%. In the other five groups, the average of α-SMA positive ratios were less than 50%. The collagen stained areas in the contused zones, concomitant with myofibroblast appearance, were increasingly augmented along with advances of posttraumatic interval. Conclusion The immunohistochemical detection of myofibroblasts can be applied to wound age determination. The myofibroblasts might be involved in collagen deposition during the repair of contused skeletal muscle in rat.展开更多
文摘1案例1.1简要案情及病史摘要某日,某孕妇因“停经39+3周,见红1 d”入院,当晚行剖宫产术娩出一足月男婴,呼吸道中清理出较多污染羊水,在继续清理呼吸道过程中出现呼吸渐弱、面色发绀,1 min Apgar评分8分。后出现继发性呼吸暂停、心率减慢,抢救约40 min后未见好转,转上级医院进一步救治。进入上级医院重症监护室,患儿面色及周身青紫,胸廓无明显隆起,呼吸急促,吸气三凹征阳性,双肺呼吸音粗,可闻及干性啰音。
基金This study was financially supported in part bythe research funds from the National Natural Science Foundation of China （81202383）, the Doctoral Program of Higher Education of China （20122104110025） and Program for Young Innovative Research Team in China University of Political Science and Law.
文摘Objective To research the relation between the time-dependent appearances of myofibroblasts during the repair of contused skeletal muscle in rat and wound age determination. Methods A total of 35 SD male rats were divided into the control and six injured groups according to wound age as follows: 12 h, 1 d, 5 d, 7 d, 10 d and 14 d after injury. The appearances of myofibroblasts were detected by HE staining, immunohistochemistry and confocal laser scanning microscopy. Masson's trichrome staining was utilized to examine collagen accumulation in the contused areas. Results Immunohistochemical staining showed that α-SMA+myofibroblasts were initially observed at 5 d post-injury. The average ratio of myofibroblasts was highest at 14 d post-injury, with all samples, ratios more than 50%. In the other five groups, the average of α-SMA positive ratios were less than 50%. The collagen stained areas in the contused zones, concomitant with myofibroblast appearance, were increasingly augmented along with advances of posttraumatic interval. Conclusion The immunohistochemical detection of myofibroblasts can be applied to wound age determination. The myofibroblasts might be involved in collagen deposition during the repair of contused skeletal muscle in rat.
