Objective: To investigate the effect of electroacupuncture(EA) at Neiguan(PC 6) on myocardial fibrosis in spontaneously hypertensive rats(SHRs), and to explore the contribution of interleukin-1β(IL-1β),insulin-like ...Objective: To investigate the effect of electroacupuncture(EA) at Neiguan(PC 6) on myocardial fibrosis in spontaneously hypertensive rats(SHRs), and to explore the contribution of interleukin-1β(IL-1β),insulin-like growth factor 1(IGF-1), and transforming growth factor β1(TGF-β1) to the effects. Methods:Nine 12-weeks-old Wistar Kyoto(WKY) male rats were employed as the normal group. Twenty-seven SHRs were equally randomized into SHR, SHR+EA, and SHR + sham groups. EA was applied at bilateral PC 6once a day 30 min per day in 8 consecutive weeks. After 8-weeks EA treatment at PC 6, histopathologic changes of collagen type Ⅰ(Col Ⅰ), collagen type Ⅲ(Col Ⅲ) and the levels of IGF-1, 1L-1β, TGF-β1,matrix metalloproteinase(MMP)-2 and MMP-9 were examined in myocardial tissure respectively. Results:After 8-weeks EA treatment at PC 6, the enhanced myocardial fibrosis in SHRs were characterized by the increased mean fluorescence intensity of Col Ⅰ and Col Ⅲ in myocardium tissue(P<0.01). All these abnormal alterations above in SHR + EA group was significantly lower compared with the SHR group(P<0.01). Meanwhile,the increased levels of IL-1β, IGF-1, TGF-β1 in serum or myocardial tissue of SHRs, diminished MMP 9mRNA expression in SHRs were also markedly inhibited after 8 weeks of EA treatment(P<0.05 or P<0.01).Furthermore, the contents of IL-1β, IGF-1, TGF-β1 in myocardial tissue were positively correlated with the systolic blood pressure and hydroxyproline respectively(P<0.01). Conclusion: EA at bilateral PC 6 could ameliorate cardiac fibrosis in SHRs, which might be mediated by regulation of 1L-1β/IGF-1-TGF-β1-MMP9 pathway.展开更多
基金Supported by the National Natural Science Foundation of China(No.81804211,No.81774439)China Academy of Chinese Medical Sciences Foundation(No.ZZ13-YQ-065)。
文摘Objective: To investigate the effect of electroacupuncture(EA) at Neiguan(PC 6) on myocardial fibrosis in spontaneously hypertensive rats(SHRs), and to explore the contribution of interleukin-1β(IL-1β),insulin-like growth factor 1(IGF-1), and transforming growth factor β1(TGF-β1) to the effects. Methods:Nine 12-weeks-old Wistar Kyoto(WKY) male rats were employed as the normal group. Twenty-seven SHRs were equally randomized into SHR, SHR+EA, and SHR + sham groups. EA was applied at bilateral PC 6once a day 30 min per day in 8 consecutive weeks. After 8-weeks EA treatment at PC 6, histopathologic changes of collagen type Ⅰ(Col Ⅰ), collagen type Ⅲ(Col Ⅲ) and the levels of IGF-1, 1L-1β, TGF-β1,matrix metalloproteinase(MMP)-2 and MMP-9 were examined in myocardial tissure respectively. Results:After 8-weeks EA treatment at PC 6, the enhanced myocardial fibrosis in SHRs were characterized by the increased mean fluorescence intensity of Col Ⅰ and Col Ⅲ in myocardium tissue(P<0.01). All these abnormal alterations above in SHR + EA group was significantly lower compared with the SHR group(P<0.01). Meanwhile,the increased levels of IL-1β, IGF-1, TGF-β1 in serum or myocardial tissue of SHRs, diminished MMP 9mRNA expression in SHRs were also markedly inhibited after 8 weeks of EA treatment(P<0.05 or P<0.01).Furthermore, the contents of IL-1β, IGF-1, TGF-β1 in myocardial tissue were positively correlated with the systolic blood pressure and hydroxyproline respectively(P<0.01). Conclusion: EA at bilateral PC 6 could ameliorate cardiac fibrosis in SHRs, which might be mediated by regulation of 1L-1β/IGF-1-TGF-β1-MMP9 pathway.
