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Characteristics of the dynamic changes in active accumulated temperature in Sichuan,China in the last 51 years against the background of climate change
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作者 WANG Hao JIANG Shan +3 位作者 WANG Jia-bin yu xiao-hang HUANG Jia-ning LIU Jian-gang 《Journal of Mountain Science》 SCIE CSCD 2023年第1期115-128,共14页
It is of utmost necessity to understand the dynamics of regional active accumulated temperature(AAT)to cope with the negative impacts of global warming on agroforestry development and food security and to provide a re... It is of utmost necessity to understand the dynamics of regional active accumulated temperature(AAT)to cope with the negative impacts of global warming on agroforestry development and food security and to provide a real-time and effective reference basis for regional agroforestry planning.The daily temperature data from 30 meteorological stations in Sichuan Province from 1970 to 2020,and sea surface temperature(SST)index data from the Atlantic Multiphase Oscillation(AMO)and Pacific Decadal Oscillation(PDO)were used for the study.Sichuan Province was divided into the western region(WS)and the eastern region(ES),considering 1000 m above sea level as the boundary.The spatiotemporal characteristics of≥0℃ and≥10℃ active accumulated temperature(AAT0,AAT10)in WS and ES were analyzed comprehensively using 5-day average sliding,empirical orthogonal function(EOF),ensemble empirical mode decomposition(EEMD),and multiple mutation tests.The results show that(1)AAT0 and AAT10 of WS ranged from 3034℃ to 3586℃ and 1971℃ to 2636℃,respectively,while the AAT0 and AAT10 of ES ranged from 5863℃ to 6513℃ and 4847℃ to 5875℃,respectively.The period around 1997 was a significant abrupt change,and the AAT in the province generally increased during the subsequent time period(2)AAT in the study area is mainly driven by the fluctuations of AMO,as reflected by the low-to-high variation of AAT coinciding with the jump of the cold-to-warm phase of AMO.Considering different time scale fluctuations in the past 51 years,the major cycle for both AAT0 and AAT10 in WS is 3.40 a,while the major cycles in ES are 3.64 a and 3.19 a,respectively with a sub-cycle of 7.29 a.AAT fluctuation has an insignificant periodic characteristic of 25.50 a on the interdecadal scale(3)The spatial heterogeneity of AAT in WS is prominent and is mainly reflected by the significantly warm conditions in the south of the WS region and relatively slight warm conditions in the north,as well as by the isolated cooling area in the form of"freezing point",i.e.,Xiaojin county.In contrast,the spatial variability of AAT in ES is more or less consistent,with the warming areas concentrated in the foothills of the western edge of the basin and a slight increase in AAT observed in the central part of the basin. 展开更多
关键词 Sichuan Province Active accumulated temperature(AAT) Ensemble empirical mode decomposition(EEMD) Mutation analysis Empirical orthogonal function(EOF)
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美国白蛾热激蛋白70基因的鉴定及功能分析 被引量:2
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作者 乔恒 李慧 +3 位作者 耿薏舒 赵旭东 于晓航 郝德君 《昆虫学报》 CAS CSCD 北大核心 2021年第7期790-799,共10页
【目的】探究热激蛋白70(heat shock protein 70,HSP70)基因在美国白蛾Hyphantria cunea抵御高温胁迫过程中的作用,为揭示美国白蛾的扩散机制以及预测潜在分布区提供理论支撑。【方法】利用PCR技术克隆美国白蛾HSP70基因,并进行生物信... 【目的】探究热激蛋白70(heat shock protein 70,HSP70)基因在美国白蛾Hyphantria cunea抵御高温胁迫过程中的作用,为揭示美国白蛾的扩散机制以及预测潜在分布区提供理论支撑。【方法】利用PCR技术克隆美国白蛾HSP70基因,并进行生物信息学分析;利用qPCR技术检测新蜕皮的美国白蛾4龄第2天幼虫在25,30,35和40℃处理下HSP70基因的表达特性;构建美国白蛾HSP70的原核表达载体,并在大肠杆菌Escherichia coli BL21感受态细胞中诱导表达该蛋白,利用Ni2+-His柱纯化蛋白,并通过Western blot验证该蛋白;利用体外实验测定原核表达获得的重组蛋白在高温胁迫下的ATP酶活性。【结果】克隆获得美国白蛾2条HSP70基因HcHSP70(GenBank登录号:MT995848)和HcHSC70(GenBank登录号:MT261583),其ORF长度分别为1917和2061 bp,分别编码637和687个氨基酸,分子质量分别约为69.66和74.96 kD,等电点分别为5.90和5.96;氨基酸序列结构均含有3个高度保守的区域GIDLGTTYS,IFDLGGGTFDVSIL和VGGSTRIPKVQ,符合热激蛋白70家族的特征;蛋白三维结构均由N端ATPase功能域和C端底物结合功能域所组成。系统进化树显示HcHSP70与鳞翅目HSP70家族其他成员聚为一支,而HcHSC70与鳞翅目HSC70家族的其他成员聚为另一支。qPCR结果表明,新蜕皮的美国白蛾4龄第2天幼虫在热胁迫下HcHSP70的相对表达量显著上调,且在35℃处理2 h下达到峰值,而热胁迫下HcHSC70的表达响应较微弱。成功构建了HcHSP70的原核表达载体并在体外诱导表达。纯化后的重组蛋白HcHSP70具备ATPase活性,且在高温胁迫下酶活性稳定。【结论】本研究克隆获得美国白蛾2条HSP70基因HcHSP70和HcHSC70,明确了两条基因在高温处理下的表达特性;成功对美国白蛾HcHSP70进行原核表达及纯化,并证明重组蛋白HcHSP70在高温下具有稳定的ATPase活性,推测其在美国白蛾应对高温胁迫中发挥着重要作用。 展开更多
关键词 美国白蛾 热激蛋白70 高温胁迫 原核表达 分子伴侣
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提高阳极炉直收率的研究
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作者 虞晓航 马国跃 谢长振 《广州化工》 CAS 2022年第13期173-175,共3页
直收率是阳极板铜含量与直接投入原料铜含量的百分比,检验的是冶金工艺的效率。理论分析造成阳极炉直收率低的原因,提出了提高阳极炉直收率具体办法有:合理搭配入炉铜料、控制炉内温度、控制加入溶剂量、吹风时间和还原时间适当。采取... 直收率是阳极板铜含量与直接投入原料铜含量的百分比,检验的是冶金工艺的效率。理论分析造成阳极炉直收率低的原因,提出了提高阳极炉直收率具体办法有:合理搭配入炉铜料、控制炉内温度、控制加入溶剂量、吹风时间和还原时间适当。采取相对的控制手段,减少铜的损失,提高阳极炉的直收率。目前,通过此方式直收率已明显提高,铜金属的价格贵,相对应的提高直收率能够明显的提高经济效益。 展开更多
关键词 直收率 阳极炉
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