Moderate leaf rolling can maintain leaf erectness,improve light transmittance in the population,and improve light energy utilization,thereby increasing rice yield.This study used ethyl methanesulfonate(EMS)to treat Yu...Moderate leaf rolling can maintain leaf erectness,improve light transmittance in the population,and improve light energy utilization,thereby increasing rice yield.This study used ethyl methanesulfonate(EMS)to treat Yunjing 17(YJ17)and obtained a semi-rolled leaf mutant that was named semi-rolled leaf 3(srl3).We found that the rolled-leaf phenotype was due to the aberrant development of bulliform cells and the loss of sclerenchymatous cells.In addition,the shoot and root length of srl3 seedlings differed from the wild type.The srl3 mutant had significantly lower plant height and seed-setting rate but notably greater tiller number,panicle length,and primary branch number per panicle than the wild type.Genetic analysis showed that a single recessive nuclear gene defined the srl3 mutant,and it was precisely located in a 144-kb region between two insertion-deletion(InDel)markers,M8 and M19,on chromosome 2.In this region,no leaf-rollingrelated genes have been reported previously.Thus,the study indicated that SRL3 is a novel leaf-rolling-related gene,and the results laid the foundation for the cloning and functional analysis of the SRL3 gene.展开更多
Two novel macrocyclic Schiff base complexes[LnL(PhCOO)(CH3OH)](ClO4)2·(CH3OH)2(Ln=Ho,1;Ln=Er,2),in which L denotes the macrocyclic ligand,were synthesized via condensation of 2,6-diformyl-4-methyl-phenol and 1,3-...Two novel macrocyclic Schiff base complexes[LnL(PhCOO)(CH3OH)](ClO4)2·(CH3OH)2(Ln=Ho,1;Ln=Er,2),in which L denotes the macrocyclic ligand,were synthesized via condensation of 2,6-diformyl-4-methyl-phenol and 1,3-diamino-2-propanol by employing benzoate as the second ligand.Their crystal structures were determined by X-ray diffraction,which showed that both complexes belong to a monoclinic system with space group P21/c.The coordination polyhedron displayed a distorted bicapped trigonal prism with the coordination number of eight.Both complexes efficiently cleaved supercoiled DNA to its nicked circular form.The UV-vis absorption results and ethidium bromide(EB)fluorescence spectra indicated that the complexes bind to CT-DNA in an intercalative mode,with Ksq values to be 11830 and 10560,respectively.展开更多
基金supported by the National Natural Science Foundation of China(32071993 and 91735304)。
文摘Moderate leaf rolling can maintain leaf erectness,improve light transmittance in the population,and improve light energy utilization,thereby increasing rice yield.This study used ethyl methanesulfonate(EMS)to treat Yunjing 17(YJ17)and obtained a semi-rolled leaf mutant that was named semi-rolled leaf 3(srl3).We found that the rolled-leaf phenotype was due to the aberrant development of bulliform cells and the loss of sclerenchymatous cells.In addition,the shoot and root length of srl3 seedlings differed from the wild type.The srl3 mutant had significantly lower plant height and seed-setting rate but notably greater tiller number,panicle length,and primary branch number per panicle than the wild type.Genetic analysis showed that a single recessive nuclear gene defined the srl3 mutant,and it was precisely located in a 144-kb region between two insertion-deletion(InDel)markers,M8 and M19,on chromosome 2.In this region,no leaf-rollingrelated genes have been reported previously.Thus,the study indicated that SRL3 is a novel leaf-rolling-related gene,and the results laid the foundation for the cloning and functional analysis of the SRL3 gene.
基金supported by the Natural Science Foundation of Hubei Province (2018CFB323)。
文摘Two novel macrocyclic Schiff base complexes[LnL(PhCOO)(CH3OH)](ClO4)2·(CH3OH)2(Ln=Ho,1;Ln=Er,2),in which L denotes the macrocyclic ligand,were synthesized via condensation of 2,6-diformyl-4-methyl-phenol and 1,3-diamino-2-propanol by employing benzoate as the second ligand.Their crystal structures were determined by X-ray diffraction,which showed that both complexes belong to a monoclinic system with space group P21/c.The coordination polyhedron displayed a distorted bicapped trigonal prism with the coordination number of eight.Both complexes efficiently cleaved supercoiled DNA to its nicked circular form.The UV-vis absorption results and ethidium bromide(EB)fluorescence spectra indicated that the complexes bind to CT-DNA in an intercalative mode,with Ksq values to be 11830 and 10560,respectively.
