通过挖掘水稻籽粒中锌含量显著关联的QTL,并结合基因功能注释、单倍型和灌浆期籽粒相对表达量分析,筛选与水稻籽粒锌含量相关的候选基因。本研究以139份西北早粳稻核心种质资源为试验材料,结合重测序获得的1800920个高可信度SNPs,基于...通过挖掘水稻籽粒中锌含量显著关联的QTL,并结合基因功能注释、单倍型和灌浆期籽粒相对表达量分析,筛选与水稻籽粒锌含量相关的候选基因。本研究以139份西北早粳稻核心种质资源为试验材料,结合重测序获得的1800920个高可信度SNPs,基于一般线性模型(GLM,general linear model)进行全基因组关联分析,对显著QTL区间内候选基因进行功能注释,并对进一步筛选后的基因进行单倍型和籽粒灌浆期相对表达量分析,以期筛选出与籽粒锌含量相关的候选基因和优势单倍型。结果表明,139份水稻中籽粒锌含量存在丰富的表型变异,且符合正态分布。全基因组关联分析共关联到12个显著SNP,分布在第1、2、4、10、11号染色体上,结合候选区间内的基因功能注释,推测定位在囊泡中的OsFRDL2可能与水稻籽粒锌元素富集相关;单倍型和灌浆期籽粒相对表达量分析表明,OsFRDL2在优势单倍型Hap5中表达量相对较高。因此推测OsFRDL2可能与水稻籽粒锌元素富集相关。研究结果为解析水稻籽粒锌元素富集机制和开发富锌水稻分子标记提供理论基础,为培育富锌水稻提供基因资源。展开更多
Rice is an important food crop grown in more than 100 countries,and is of particular significance for the Asian people.Obtaining rice cultivars characterized by high yield and outstanding quality has always been the d...Rice is an important food crop grown in more than 100 countries,and is of particular significance for the Asian people.Obtaining rice cultivars characterized by high yield and outstanding quality has always been the desire of rice breeders and scientists.Starch is the principal source of human energy from plant foods.The starch in rice consists of mainly amylose,amylopectin and resistant starch(RS).Amylose and amylopectin are forms of starch that are easily digested in the small intestine,and play an important role in defining the quality of rice.RS is a new form of dietary fiber not easily degraded into glucose in the small intestine which effectively reduces postprandial blood glucose concentration,so it has a significant effect on the nutritional quality of rice.This paper discusses the current gene function research of these three starches in rice,analyzes the effects of some abiotic stress on rice starch content and structure,and explores the relationship between them in order to provide a theoretical reference for the cultivation of new high-quality,high-RS content rice varieties.展开更多
文摘通过挖掘水稻籽粒中锌含量显著关联的QTL,并结合基因功能注释、单倍型和灌浆期籽粒相对表达量分析,筛选与水稻籽粒锌含量相关的候选基因。本研究以139份西北早粳稻核心种质资源为试验材料,结合重测序获得的1800920个高可信度SNPs,基于一般线性模型(GLM,general linear model)进行全基因组关联分析,对显著QTL区间内候选基因进行功能注释,并对进一步筛选后的基因进行单倍型和籽粒灌浆期相对表达量分析,以期筛选出与籽粒锌含量相关的候选基因和优势单倍型。结果表明,139份水稻中籽粒锌含量存在丰富的表型变异,且符合正态分布。全基因组关联分析共关联到12个显著SNP,分布在第1、2、4、10、11号染色体上,结合候选区间内的基因功能注释,推测定位在囊泡中的OsFRDL2可能与水稻籽粒锌元素富集相关;单倍型和灌浆期籽粒相对表达量分析表明,OsFRDL2在优势单倍型Hap5中表达量相对较高。因此推测OsFRDL2可能与水稻籽粒锌元素富集相关。研究结果为解析水稻籽粒锌元素富集机制和开发富锌水稻分子标记提供理论基础,为培育富锌水稻提供基因资源。
基金the Key R&D Plan Project of Ningxia(Talent Project)(2019BEB04014)the Agricultural Breeding Project of Ningxia(2018NYYZ0302)the Scientific Research Funding Project of Ningxia University(ZR18010)。
文摘Rice is an important food crop grown in more than 100 countries,and is of particular significance for the Asian people.Obtaining rice cultivars characterized by high yield and outstanding quality has always been the desire of rice breeders and scientists.Starch is the principal source of human energy from plant foods.The starch in rice consists of mainly amylose,amylopectin and resistant starch(RS).Amylose and amylopectin are forms of starch that are easily digested in the small intestine,and play an important role in defining the quality of rice.RS is a new form of dietary fiber not easily degraded into glucose in the small intestine which effectively reduces postprandial blood glucose concentration,so it has a significant effect on the nutritional quality of rice.This paper discusses the current gene function research of these three starches in rice,analyzes the effects of some abiotic stress on rice starch content and structure,and explores the relationship between them in order to provide a theoretical reference for the cultivation of new high-quality,high-RS content rice varieties.
