Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectr...Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum. The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%, This result indicates the tryptophan residue is essential for the interaction between ssDNA and IgGRF .and ionic-bonding plays an important role in adsorption.展开更多
Stroma--free hemoglobin (SFHb) was prepared from fresh or out--dated whole blood by lysing red cells. At different temperatures, pyridoxal 5--phosphate (PLP) was added in a 4:1 molar-ratio to the Hb tetramer followed ...Stroma--free hemoglobin (SFHb) was prepared from fresh or out--dated whole blood by lysing red cells. At different temperatures, pyridoxal 5--phosphate (PLP) was added in a 4:1 molar-ratio to the Hb tetramer followed by reduction with NaBH4 under N2 for 16 hrs subsequenl crosslinking by glutaraldehyde in presence of lysine for 14--16 hrs. Yielded soluble pyridoxalated polyhemoglobin (Poly Hb--P). 5%--20% gradient SDS--PAGE showed the change of the Hb molecular weight (MW) after crosslinking. Gel chromatography showed that the poly Hb--P had a continuous distribution of molecular weight with the range from about 65,000 to 600,000 Dalton. The poly Hb--P solution provided a normal oxygen carrying with 23.1 of P50, at pH 7.24 or 26.1 of P50 at pH 7.24展开更多
Bioart,ficial liver support system （BALS） has the potential to provide temporary support for patients with fulminant hepatic failure and consist of viable hepatocytes and scaffolding materials for hepatocytes attach...Bioart,ficial liver support system （BALS） has the potential to provide temporary support for patients with fulminant hepatic failure and consist of viable hepatocytes and scaffolding materials for hepatocytes attachment. Various scaffolding materials are used in BALS, including chitosan, which is easily obtained by deacetylation of chitin and widely applied in biomedical applications. In this paper, we introduce and discuses chitosan-based biomaterials for BALS application.展开更多
Cholesterol modified dextran (CHD) having self-aggregation or self-assembly property was synthesized from cholesterol and 1,6-hexyldiisocyanate. The degree of substitution of cholesteryl moiety in dextran main line is...Cholesterol modified dextran (CHD) having self-aggregation or self-assembly property was synthesized from cholesterol and 1,6-hexyldiisocyanate. The degree of substitution of cholesteryl moiety in dextran main line is 3-5 cholesterols the 100 glucose units. We have prepared water solution of CHD using probe type sonifier and N-Phenyl-α-naphthylamine (PNA) as a fluorescent probe to study CHD self-aggregate process .For each solution of two samples, we found that the maximum emission of PNA in CHD solution was shifted to the lower wavelength and the intensity drastically increased above a critical CHD concentration. This change corresponds to the formation of micelle-like clusters self-aggregated by the cholesterol moiety once the CHD concentration exceeds 0.01 mg/ml.展开更多
Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on...Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on PVA-based microcarriers and cytodexIII microcarriers. Morphology, attachment and growth rate of LO2 cells were studied.展开更多
Various types of porous resins adsorbents based on polystyrene,polyvinyl alcohol,cellulose as matrixes and DNA,amino acids with other biological active molecules as ligands were extensively studied at Nankai Universit...Various types of porous resins adsorbents based on polystyrene,polyvinyl alcohol,cellulose as matrixes and DNA,amino acids with other biological active molecules as ligands were extensively studied at Nankai University.Molecular recognition between the ligand and pathogenic molecule was investigated.Several commercialized products are now widely used in hospitals all over China.Whole blood hemoperfusion are used to treat patients suffering from autoimmune diseases,uremia acute intoxication and hyperbilirubinemia.Clinical performances of hundreds and thousands of patients,treated by whole blood sorption therapy are safe,efficient(90%)and cost-effective.展开更多
A tumor-targeting gene vector G250mAb-PEI-PEG has been prepared by modification of polyethylenimine (PEI) with polyethyleneglycol (PEG) and G250, a monoclonal antibody against the G250 antigen on tumor cell surface. T...A tumor-targeting gene vector G250mAb-PEI-PEG has been prepared by modification of polyethylenimine (PEI) with polyethyleneglycol (PEG) and G250, a monoclonal antibody against the G250 antigen on tumor cell surface. The transfection efficiency was as high as 70% in G250 positive HeLa cells, whereas the transfection efficiency was relatively low (30%) in normal NIH3T3 cells. A plasmid encoding the short hairpin RNA (shRNA) specific for nucleostemin gene (NS) was efficiently transfected into the HeLa cells with this nonviral gene vector. RNA interference down-regulated the expression of NS gene in HeLa cells, inhibited cells proliferation and induced apoptosis. However, the growth and activity of the NIH3T3 cells were not affected under the same treatment. These results indicate that the reported nonviral gene vector, G250mAb-PEI-PEG, can target and efficiently deliver genes into HeLa cells, and has the potential for the cervical cancer treatment.展开更多
基金National Basic Science and Development of China (G1999O64707)
文摘Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum. The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%, This result indicates the tryptophan residue is essential for the interaction between ssDNA and IgGRF .and ionic-bonding plays an important role in adsorption.
文摘Stroma--free hemoglobin (SFHb) was prepared from fresh or out--dated whole blood by lysing red cells. At different temperatures, pyridoxal 5--phosphate (PLP) was added in a 4:1 molar-ratio to the Hb tetramer followed by reduction with NaBH4 under N2 for 16 hrs subsequenl crosslinking by glutaraldehyde in presence of lysine for 14--16 hrs. Yielded soluble pyridoxalated polyhemoglobin (Poly Hb--P). 5%--20% gradient SDS--PAGE showed the change of the Hb molecular weight (MW) after crosslinking. Gel chromatography showed that the poly Hb--P had a continuous distribution of molecular weight with the range from about 65,000 to 600,000 Dalton. The poly Hb--P solution provided a normal oxygen carrying with 23.1 of P50, at pH 7.24 or 26.1 of P50 at pH 7.24
基金The National Natural Science Foundation of China, No. 3007022 The Natural Science Foundation of Tianjin, No. 033608011
文摘Bioart,ficial liver support system （BALS） has the potential to provide temporary support for patients with fulminant hepatic failure and consist of viable hepatocytes and scaffolding materials for hepatocytes attachment. Various scaffolding materials are used in BALS, including chitosan, which is easily obtained by deacetylation of chitin and widely applied in biomedical applications. In this paper, we introduce and discuses chitosan-based biomaterials for BALS application.
文摘Cholesterol modified dextran (CHD) having self-aggregation or self-assembly property was synthesized from cholesterol and 1,6-hexyldiisocyanate. The degree of substitution of cholesteryl moiety in dextran main line is 3-5 cholesterols the 100 glucose units. We have prepared water solution of CHD using probe type sonifier and N-Phenyl-α-naphthylamine (PNA) as a fluorescent probe to study CHD self-aggregate process .For each solution of two samples, we found that the maximum emission of PNA in CHD solution was shifted to the lower wavelength and the intensity drastically increased above a critical CHD concentration. This change corresponds to the formation of micelle-like clusters self-aggregated by the cholesterol moiety once the CHD concentration exceeds 0.01 mg/ml.
基金The National Natural Science Foundation of China(No.29776027).
文摘Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on PVA-based microcarriers and cytodexIII microcarriers. Morphology, attachment and growth rate of LO2 cells were studied.
基金supported the Nankai research group for so many years
文摘Various types of porous resins adsorbents based on polystyrene,polyvinyl alcohol,cellulose as matrixes and DNA,amino acids with other biological active molecules as ligands were extensively studied at Nankai University.Molecular recognition between the ligand and pathogenic molecule was investigated.Several commercialized products are now widely used in hospitals all over China.Whole blood hemoperfusion are used to treat patients suffering from autoimmune diseases,uremia acute intoxication and hyperbilirubinemia.Clinical performances of hundreds and thousands of patients,treated by whole blood sorption therapy are safe,efficient(90%)and cost-effective.
基金Supported by the National Natural Science Foundation of China (Grant No. 20774050)National Natural Science Funds for Distinguished Young Scholar (Grant No. 30725030)National Basic Research Program of China (Grant No. 2009CB918900-G)
文摘A tumor-targeting gene vector G250mAb-PEI-PEG has been prepared by modification of polyethylenimine (PEI) with polyethyleneglycol (PEG) and G250, a monoclonal antibody against the G250 antigen on tumor cell surface. The transfection efficiency was as high as 70% in G250 positive HeLa cells, whereas the transfection efficiency was relatively low (30%) in normal NIH3T3 cells. A plasmid encoding the short hairpin RNA (shRNA) specific for nucleostemin gene (NS) was efficiently transfected into the HeLa cells with this nonviral gene vector. RNA interference down-regulated the expression of NS gene in HeLa cells, inhibited cells proliferation and induced apoptosis. However, the growth and activity of the NIH3T3 cells were not affected under the same treatment. These results indicate that the reported nonviral gene vector, G250mAb-PEI-PEG, can target and efficiently deliver genes into HeLa cells, and has the potential for the cervical cancer treatment.
