Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objecti...Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objective protein. Methods： By polymerase chain reaction （PCR） technique, SjAslp was amplified from the constructed recombinant plasmid pBCSK＋/SjAslp, and inserted into cloning vector pUCm-T. Then, SjAslp was subcloned into an eukaryotic expression vector pcDNA3.1（＋）. After identifying it by PCR, restrictive enzymes digestion and DNA sequencing, the recombinant plasmid was transfected into HeLa cells using electroporation, and the expression of the recombinant protein was analyzed by immunocytochemical assay. Results： The specific gene fragment of 558 bp was successfully amplified. The DNA vaccine of SjAslp was successfully constructed. Immunocytochemical assay showed that SjAslp was expressed in the cytoplasm of HeLa cells. Conclusion： SjAslp gene can be expressed in eukaryotic system, which lays the foundation for development of the SjAslp DNA vaccine against schitosomiasis.展开更多
Since the transition from rotating stall to surge in a transonic compressor at high speed is very quick,quite often there is no time to take measures to prevent the surge.Therefore,it is desired to find any rotating s...Since the transition from rotating stall to surge in a transonic compressor at high speed is very quick,quite often there is no time to take measures to prevent the surge.Therefore,it is desired to find any rotating stall precursors,of which the occurrence can offer sufficient time for stall or surge prevention.In this study,a series of unsteady flow analyses were performed on a transonic compressor under operating conditions before rotating stall with unsteady results scrutinized to find rotating stall precursors.Particular attention is paid to the spatial modes and time modes of static pressure near the casing and around the blade leading and trailing edges.The results show that the characteristics of the precursor in both spatial and time domains can be used as rotating stall warnings.展开更多
基金the Scientific Research Foundation of Education Committee of Hunan (07C713 and 07C708)
文摘Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objective protein. Methods： By polymerase chain reaction （PCR） technique, SjAslp was amplified from the constructed recombinant plasmid pBCSK＋/SjAslp, and inserted into cloning vector pUCm-T. Then, SjAslp was subcloned into an eukaryotic expression vector pcDNA3.1（＋）. After identifying it by PCR, restrictive enzymes digestion and DNA sequencing, the recombinant plasmid was transfected into HeLa cells using electroporation, and the expression of the recombinant protein was analyzed by immunocytochemical assay. Results： The specific gene fragment of 558 bp was successfully amplified. The DNA vaccine of SjAslp was successfully constructed. Immunocytochemical assay showed that SjAslp was expressed in the cytoplasm of HeLa cells. Conclusion： SjAslp gene can be expressed in eukaryotic system, which lays the foundation for development of the SjAslp DNA vaccine against schitosomiasis.
基金The research was supported by the National Natural Science Foundation of China under Grant No.51976172,National Science and Technology Major Project(2017-II-O009-0023),and China's 111 project under Grant No.B17037.
文摘Since the transition from rotating stall to surge in a transonic compressor at high speed is very quick,quite often there is no time to take measures to prevent the surge.Therefore,it is desired to find any rotating stall precursors,of which the occurrence can offer sufficient time for stall or surge prevention.In this study,a series of unsteady flow analyses were performed on a transonic compressor under operating conditions before rotating stall with unsteady results scrutinized to find rotating stall precursors.Particular attention is paid to the spatial modes and time modes of static pressure near the casing and around the blade leading and trailing edges.The results show that the characteristics of the precursor in both spatial and time domains can be used as rotating stall warnings.
