目的分析血小板(PLT)参数的变化及胸部CT在重症肺炎患儿中的诊断价值。方法回顾性选取2018年5月至2021年2月与本院治疗的81例重症肺炎患儿和34例普通肺炎患儿的临床资料,收集两组患儿入院第1 d PLT水平和CT检查结果,比较两组患儿PLT水平...目的分析血小板(PLT)参数的变化及胸部CT在重症肺炎患儿中的诊断价值。方法回顾性选取2018年5月至2021年2月与本院治疗的81例重症肺炎患儿和34例普通肺炎患儿的临床资料,收集两组患儿入院第1 d PLT水平和CT检查结果,比较两组患儿PLT水平,分析重症患儿CT图形特征,评价PLT水平、胸部CT检查诊断重症肺炎患儿的诊断价值。结果重症组患儿血清PLT水平明显高于轻症组患儿(P<0.05);ROC曲线显示血清PLT水平诊断重症肺炎患儿的曲线下面积为0.923,敏感度和特异度分别为77.8%和97.1%;胸部CT诊断诊断重症肺炎患儿的灵敏度、特异度和准确率分别为90.12%、88.24%、89.57%。结论PLT参数的变化和胸部CT对重症肺炎均有较高的诊断加之,临床工作中可联合应用以提高诊断准确度。展开更多
Objective: To examine the anti-inflammatory effect of grape seed extract(GSE) in animal and cellular models and explore its mechanism of action. Methods: This study determined the inhibitory effect of GSE on macrophag...Objective: To examine the anti-inflammatory effect of grape seed extract(GSE) in animal and cellular models and explore its mechanism of action. Methods: This study determined the inhibitory effect of GSE on macrophage inflammation and Th1 and Th17 polarization in vitro. Based on the in vitro results, the effects and mechanisms of GSE on multiple sclerosis(MS)-experimental autoimmune encephalomyelitis(EAE) mice model were further explored. The C57BL/6 mice were intragastrically administered with 50 mg/kg of GSE once a day from the 3rd day to the 27th day after immunization. The activation of microglia, the polarization of Th1 and Th17and the inflammatory factors such as tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6, IL-12, IL-17and interferon-γ(IFN-γ) secreted by them were detected in vitro and in vivo by flow cytometry, enzyme linked immunosorbent assay(ELISA), immunofluorescence staining and Western blot, respectively. Results: GSE reduced the secretion of TNF-α, IL-1β and IL-6 in bone marrow-derived macrophages stimulated by lipopolysaccharide(P<0.01), inhibited the secretion of TNF-α, IL-1β, IL-6, IL-12, IL-17 and IFN-γ in spleen cells of EAE mice immunized for 9 days(P<0.05 or P<0.01), and reduced the differentiation of Th1 and Th17 mediated by CD3 and CD28 factors(P<0.01). GSE significantly improved the clinical symptoms of EAE mice, and inhibited spinal cord demyelination and inflammatory cell infiltration. Peripherally, GSE downregulated the expression of toll-like-receptor 4(TLR4) and Rho-associated kinase(ROCKⅡ, P<0.05 or P<0.01), and inhibited the secretion of inflammatory factors(P<0.01 or P<0.05). In the central nervous system, GSE inhibited the infiltration of CD45+CD11b+and CD45+CD4+cells, and weakened the differentiation of Th1 and Th17(P<0.05). Moreover, it reduced the secretion of inflammatory factors(P<0.01), and prevented the activation of microglia(P<0.05). Conclusion: GSE had a beneficial effect on the pathogenesis and progression of EAE by inhibiting inflammatory response as a potential drug and strategy for the treatment of MS.展开更多
文摘目的分析血小板(PLT)参数的变化及胸部CT在重症肺炎患儿中的诊断价值。方法回顾性选取2018年5月至2021年2月与本院治疗的81例重症肺炎患儿和34例普通肺炎患儿的临床资料,收集两组患儿入院第1 d PLT水平和CT检查结果,比较两组患儿PLT水平,分析重症患儿CT图形特征,评价PLT水平、胸部CT检查诊断重症肺炎患儿的诊断价值。结果重症组患儿血清PLT水平明显高于轻症组患儿(P<0.05);ROC曲线显示血清PLT水平诊断重症肺炎患儿的曲线下面积为0.923,敏感度和特异度分别为77.8%和97.1%;胸部CT诊断诊断重症肺炎患儿的灵敏度、特异度和准确率分别为90.12%、88.24%、89.57%。结论PLT参数的变化和胸部CT对重症肺炎均有较高的诊断加之,临床工作中可联合应用以提高诊断准确度。
基金Supported by the National Natural Science Foundation of China (No.81903596)Science and Technology Innovation Project of Shanxi Colleges (No.2019L0728)+2 种基金Leading Team of Medical Science and Technology of Shanxi Province (No.2020TD05)Funds for Construction of Key Disciplines from Shanxi University of Chinese Medicine (No.030200117)Cultivation Project of Shanxi University of Chinese Medicine (Nos.2019PY130 and 2020PY-JC-14)。
文摘Objective: To examine the anti-inflammatory effect of grape seed extract(GSE) in animal and cellular models and explore its mechanism of action. Methods: This study determined the inhibitory effect of GSE on macrophage inflammation and Th1 and Th17 polarization in vitro. Based on the in vitro results, the effects and mechanisms of GSE on multiple sclerosis(MS)-experimental autoimmune encephalomyelitis(EAE) mice model were further explored. The C57BL/6 mice were intragastrically administered with 50 mg/kg of GSE once a day from the 3rd day to the 27th day after immunization. The activation of microglia, the polarization of Th1 and Th17and the inflammatory factors such as tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6, IL-12, IL-17and interferon-γ(IFN-γ) secreted by them were detected in vitro and in vivo by flow cytometry, enzyme linked immunosorbent assay(ELISA), immunofluorescence staining and Western blot, respectively. Results: GSE reduced the secretion of TNF-α, IL-1β and IL-6 in bone marrow-derived macrophages stimulated by lipopolysaccharide(P<0.01), inhibited the secretion of TNF-α, IL-1β, IL-6, IL-12, IL-17 and IFN-γ in spleen cells of EAE mice immunized for 9 days(P<0.05 or P<0.01), and reduced the differentiation of Th1 and Th17 mediated by CD3 and CD28 factors(P<0.01). GSE significantly improved the clinical symptoms of EAE mice, and inhibited spinal cord demyelination and inflammatory cell infiltration. Peripherally, GSE downregulated the expression of toll-like-receptor 4(TLR4) and Rho-associated kinase(ROCKⅡ, P<0.05 or P<0.01), and inhibited the secretion of inflammatory factors(P<0.01 or P<0.05). In the central nervous system, GSE inhibited the infiltration of CD45+CD11b+and CD45+CD4+cells, and weakened the differentiation of Th1 and Th17(P<0.05). Moreover, it reduced the secretion of inflammatory factors(P<0.01), and prevented the activation of microglia(P<0.05). Conclusion: GSE had a beneficial effect on the pathogenesis and progression of EAE by inhibiting inflammatory response as a potential drug and strategy for the treatment of MS.
