Based on geochemical analysis results of core samples from the Triassic Chang 7 Member of Well Feng 75 drilled in the northwest margin of Ordos Basin,combined with geological characteristics of this region,the formati...Based on geochemical analysis results of core samples from the Triassic Chang 7 Member of Well Feng 75 drilled in the northwest margin of Ordos Basin,combined with geological characteristics of this region,the formation environment of the black shale and its control on shale oil enrichment are comprehensively studied.From the Chang 73 to Chang 71 Sub-members,the black shale have organic carbon contents decreasing from 5.70%to 3.55%,hydrogen indexes decreasing from 345 mg/g to 269 mg/g,while the oxygen indexes increasing gradually from 6 mg/g to 29 mg/g,indicating that the sedimentary environment during the depositional period of Chang 72 and Chang 73 Sub-members was anoxic.Biomarkers in the black shale change regu-larly,and have an obvious“inflection point”at the depth of 2753–2777 m in the Chang 73 Sub-member,indicating that the input of terrigenous organic matter increased.However,there is a negative drift about 2%of organic carbon isotopic composition near the“inflection point”,which is in conflict with the results of biomarker compounds.This is because the extreme thermal and anoxic events caused by continental volcanic activity in the ancient Qinling region caused negative drift of carbon isotopic composition of the black shale in the Ordos Basin.The volcanic activity caused rise of temperature,polluted air,extreme weathers,more floods and thus more input of terrigenous organic matter,and gave rise to extremely anoxic environment con-ducive to the preservation of organic matter.Terrigenous organic matter is more conducive to the formation of light oil than aquatic organic matter,so these sections in Yanchang Formation are major“sweet spots”for shale oil enrichment.展开更多
目的利用成簇的规律间隔短回文重复序列和相关蛋白9(clustered regular interval short palindromic repeats/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术敲除长白猪胎儿成纤维细胞(porcine fetal fibroblasts,PFFs)的PIN1基...目的利用成簇的规律间隔短回文重复序列和相关蛋白9(clustered regular interval short palindromic repeats/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术敲除长白猪胎儿成纤维细胞(porcine fetal fibroblasts,PFFs)的PIN1基因,以构建PIN1基因敲除长白猪胎儿成纤维细胞系。方法对人和猪的PIN1基因进行同源性分析。利用在线工具设计2个靶向猪PIN1基因第二个外显子区的sgRNAs,并克隆至pX330骨架质粒中。将构建成功的打靶质粒和Neomycin抗性质粒共转染至PFFs中,用G418药物筛选出抗性单细胞克隆,测序鉴定其基因型并从转录和翻译水平鉴定PIN1的表达。结果同源性分析显示人和猪PIN1蛋白的氨基酸序列一致性和相似性均为98%,二者三维结构的均方根偏差(RMSD)值为0.014。获得15个PIN1基因敲除的纯合单克隆细胞系,并且证实PIN1蛋白在翻译水平被完全敲除。结论利用双sgRNAs引导的CRSIPR/Cas9系统在PFFs中高效实现PIN1基因的双等位基因敲除,成功建立PIN1基因敲除的长白猪胎儿成纤维细胞系。展开更多
The influence of water on gas generation from humic type organic matter at highly to over mature stage was investigated with thermal simulation experiments at high temperature and pressure.The result of the experiment...The influence of water on gas generation from humic type organic matter at highly to over mature stage was investigated with thermal simulation experiments at high temperature and pressure.The result of the experiments indicates that the effect of water on gas generation was controlled by the thermal maturity of organic matter.Water could enhance gas generation and increase hydrocarbon gas yields significantly at over mature stage of humic type organic matter.Hydrogen isotopic compositions of coal-derived gases generated at highly to over mature stage were mainly controlled by thermal maturity of source rocks,but also affected by formation water.Highly and over mature coal measure source rocks are widely distributed in China.The hydrocarbon gas generation capacity of coal measure source rocks and resource potential of coal-derived gases in deep formations would be significantly enhanced assuming that formation water could be involved in the thermal cracking of highly to over mature organic matter in real geological settings.展开更多
基金Supported by the National Science and Technology Major Project(2016ZX05050)Scientific Research and Technology Development Program of the Research Institute of Petroleum Exploration and Development,Petrochina(No.2018YCQ02)。
文摘Based on geochemical analysis results of core samples from the Triassic Chang 7 Member of Well Feng 75 drilled in the northwest margin of Ordos Basin,combined with geological characteristics of this region,the formation environment of the black shale and its control on shale oil enrichment are comprehensively studied.From the Chang 73 to Chang 71 Sub-members,the black shale have organic carbon contents decreasing from 5.70%to 3.55%,hydrogen indexes decreasing from 345 mg/g to 269 mg/g,while the oxygen indexes increasing gradually from 6 mg/g to 29 mg/g,indicating that the sedimentary environment during the depositional period of Chang 72 and Chang 73 Sub-members was anoxic.Biomarkers in the black shale change regu-larly,and have an obvious“inflection point”at the depth of 2753–2777 m in the Chang 73 Sub-member,indicating that the input of terrigenous organic matter increased.However,there is a negative drift about 2%of organic carbon isotopic composition near the“inflection point”,which is in conflict with the results of biomarker compounds.This is because the extreme thermal and anoxic events caused by continental volcanic activity in the ancient Qinling region caused negative drift of carbon isotopic composition of the black shale in the Ordos Basin.The volcanic activity caused rise of temperature,polluted air,extreme weathers,more floods and thus more input of terrigenous organic matter,and gave rise to extremely anoxic environment con-ducive to the preservation of organic matter.Terrigenous organic matter is more conducive to the formation of light oil than aquatic organic matter,so these sections in Yanchang Formation are major“sweet spots”for shale oil enrichment.
文摘目的利用成簇的规律间隔短回文重复序列和相关蛋白9(clustered regular interval short palindromic repeats/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术敲除长白猪胎儿成纤维细胞(porcine fetal fibroblasts,PFFs)的PIN1基因,以构建PIN1基因敲除长白猪胎儿成纤维细胞系。方法对人和猪的PIN1基因进行同源性分析。利用在线工具设计2个靶向猪PIN1基因第二个外显子区的sgRNAs,并克隆至pX330骨架质粒中。将构建成功的打靶质粒和Neomycin抗性质粒共转染至PFFs中,用G418药物筛选出抗性单细胞克隆,测序鉴定其基因型并从转录和翻译水平鉴定PIN1的表达。结果同源性分析显示人和猪PIN1蛋白的氨基酸序列一致性和相似性均为98%,二者三维结构的均方根偏差(RMSD)值为0.014。获得15个PIN1基因敲除的纯合单克隆细胞系,并且证实PIN1蛋白在翻译水平被完全敲除。结论利用双sgRNAs引导的CRSIPR/Cas9系统在PFFs中高效实现PIN1基因的双等位基因敲除,成功建立PIN1基因敲除的长白猪胎儿成纤维细胞系。
基金Supported by the National Natural Science Foundation of China(41472120)
文摘The influence of water on gas generation from humic type organic matter at highly to over mature stage was investigated with thermal simulation experiments at high temperature and pressure.The result of the experiments indicates that the effect of water on gas generation was controlled by the thermal maturity of organic matter.Water could enhance gas generation and increase hydrocarbon gas yields significantly at over mature stage of humic type organic matter.Hydrogen isotopic compositions of coal-derived gases generated at highly to over mature stage were mainly controlled by thermal maturity of source rocks,but also affected by formation water.Highly and over mature coal measure source rocks are widely distributed in China.The hydrocarbon gas generation capacity of coal measure source rocks and resource potential of coal-derived gases in deep formations would be significantly enhanced assuming that formation water could be involved in the thermal cracking of highly to over mature organic matter in real geological settings.
