Objectives: In this study, bibliometric approach was used to explore the literature in the field of research related to Diabetic Myasthenia Gravis in order to identify the current research progress in Diabetic Myasthe...Objectives: In this study, bibliometric approach was used to explore the literature in the field of research related to Diabetic Myasthenia Gravis in order to identify the current research progress in Diabetic Myasthenia Gravis related research and to help the researchers to predict the future hotspots in the field of research and to provide reference for the research. Methods: Literature related to diabetic sarcopenia published from 1993 to 2023 since the inception of the repository was extracted from the Web of Science Core Collection (WoSCC) and bibliometric analyses were performed. We have analysed the published literature of the last approximately almost 30 years, as well as publication and citation analyses from different countries, institutions, journals and authors. For keywords, we performed co-occurrence, clustering, timeline view and citation burst analysis. Results: On the basis of 1564 publications, we found a continuous increase in the number of publications and citations, especially in the last six years. The United States is the most representative country, and Seoul National University (SNU) is the most representative institution. The most popular journal in the field is Diabetes Care;Fukui, Michiaki is the most prolific author, leading many studies related to diabetic sarcopenia. The most frequently cited reference was a revised European consensus on the definition and diagnosis of sarcopenia;the most cited keywords were related to physiological factors of diabetes, sarcopenia and related conditions, such as “insulin resistance”, “skeletal muscle”, “body composition”, “risk” and “prevalence”. Conclusion: With more and more studies on the relationship between diabetic sarcopenia, this study presents the current status and trend of research related to diabetic sarcopenia over the past nearly 30 years through the visualization software CiteSpace. It can help researchers identify potential collaborators and partner institutions, hotspots and research frontiers in the field of diabetic sarcopenia. However, our work is only based on the English language literature in the WoSCC database, and for future studies, we recommend that researchers explore the literature from multiple databases to enhance the scope of their research.展开更多
Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein re...Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response inβcells remains unclear.Methods:To investigate this,we induced early-onset T1D in non-obese diabetic mice using streptozotocin.Subsequently,T1D mice were randomly assigned to receive either MSCs or phosphate-buffered saline.We observed the in vivo homing of MSCs and assessed their effectiveness by analyzing blood glucose levels,body weight,histopathology,pancreatic protein expression,and serum levels of cytokines,proinsulin,and C-peptide.Results:Infused MSCs were found in the lungs,liver,spleen,and pancreas of T1D mice.They exhibited various effects,including reducing blood glucose levels,regulating immunity,inhibiting inflammation,increasingβ-cell areas,and reducing the expression of key proteins in the unfolded protein response pathway.Fasting serum proinsulin and C-peptide levels were significantly higher in the MSCs treatment group than in the T1D model group.However,there was no significant difference in the biomarker ofβ-cell endoplasmic reticulum stress,the ratio of fasting serum proinsulin to C-peptide,between the two groups.Conclusion:Ourfindings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress inβcells directly but modulates the unfolded protein response pathway to preserveβ-cell mass and function in T1D mice.展开更多
在自动驾驶场景下的3D目标检测任务中,探索毫米波雷达数据作为RGB图像输入的补充正成为多模态融合的新兴趋势。然而,现有的毫米波雷达-相机融合方法高度依赖于相机的一阶段检测结果,导致整体性能不够理想。本文提供了一种不依赖于相机...在自动驾驶场景下的3D目标检测任务中,探索毫米波雷达数据作为RGB图像输入的补充正成为多模态融合的新兴趋势。然而,现有的毫米波雷达-相机融合方法高度依赖于相机的一阶段检测结果,导致整体性能不够理想。本文提供了一种不依赖于相机检测结果的鸟瞰图下双向融合方法(BEV-radar)。对于来自不同域的两个模态的特征,BEV-radar设计了一个双向的基于注意力的融合策略。具体地,以基于BEV的3D目标检测方法为基础,我们的方法使用双向转换器嵌入来自两种模态的信息,并根据后续的卷积块强制执行局部空间关系。嵌入特征后,BEV特征在3D对象预测头中解码。我们在nu Scenes数据集上评估了我们的方法,实现了48.2 m AP和57.6 NDS。结果显示,与仅使用相机的基础模型相比,不仅在精度上有所提升,特别地,速度预测误差项有了相当大的改进。代码开源于https://github.com/Etah0409/BEV-Radar。展开更多
The couple between the power network and the transportation network(TN)is deepening gradually with the increasing penetration rate of electric vehicles(EV),which also poses a great challenge to the traditional voltage...The couple between the power network and the transportation network(TN)is deepening gradually with the increasing penetration rate of electric vehicles(EV),which also poses a great challenge to the traditional voltage control scheme.In this paper,we propose a coordinated voltage control strategy for the active distribution networks considering multiple types of EV.In the first stage,the action of on-load tap changer and capacitor banks,etc.,are determined by optimal power flow calculation,and the node electricity price is also determined based on dynamic time-of-use tariff mechanism.In the second stage,multiple operating scenarios of multiple types of EVs such as cabs,private cars and buses are considered,and the scheduling results of each EV are solved by building an optimization model based on constraints such as queuing theory,Floyd-Warshall algorithm and traffic flow information.In the third stage,the output power of photovoltaic and energy storage systems is fine-tuned in the normal control mode.The charging power of EVs is also regulated in the emergency control mode to reduce the voltage deviation,and the amount of regulation is calculated based on the fair voltage control mode of EVs.Finally,we test the modified IEEE 33-bus distribution system coupled with the 24-bus Beijing TN.The simulation results show that the proposed scheme can mitigate voltage violations well.展开更多
BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the role of TMEM100 in esophageal cancer(EC).AIM To investigate epigenetic regulation of TMEM100 expression in ESCC and the effect of TMEM100 on ESCC proliferation and invasion.METHODS Firstly,we found the expression of TMEM100 in EC through The Cancer Genome Atlas database.The correlation between TMEM100 gene expression and the survival of patients with EC was further confirmed through Kaplan-Meier analysis.We then added the demethylating agent 5-AZA to ESCC cell lines to explore the regulation of TMEM100 expression by epigenetic modification.To observe the effect of TMEM100 expression on tumor proliferation and invasion by overexpressing TMEM100.Finally,we performed gene set enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes Orthology-Based Annotation System database to look for pathways that might be affected by TMEM100 and verified the effect of TMEM100 expression on the mitogen-activated protein kinases(MAPK)pathway.RESULTS In the present study,by bioinformatic analysis we found that TMEM100 was lowly expressed in EC patients compared to normal subjects.Kaplan-meier survival analysis showed that low expression of TMEM100 was associated with poor prognosis in patients with EC.Then,we found that the demethylating agent 5-AZA resulted in increased expression of TMEM100 in ESCC cells[quantitative real-time PCR(qRT-PCR)and western blotting].Subsequently,we confirmed that overexpression of TMEM100 leads to its increased expression in ESCC cells(qRT-PCR and western blotting).Overexpression of TMEM100 also inhibited proliferation,invasion and migration of ESCC cells(cell counting kit-8 and clone formation assays).Next,by enrichment analysis,we found that the gene set was significantly enriched in the MAPK signaling pathway.The involvement of TMEM100 in the regulation of MAPK signaling pathway in ESCC cell was subsequently verified by western blotting.CONCLUSION TMEM100 is a suppressor gene in ESCC,and its low expression may lead to aberrant activation of the MAPK pathway.Promoter methylation may play a key role in regulating TMEM100 expression.展开更多
In recent years, sarcopenia, as a progressive muscular atrophy and weakness, has become one of the common diseases in the elderly. Although its cause is not fully understood, a growing body of research suggests that e...In recent years, sarcopenia, as a progressive muscular atrophy and weakness, has become one of the common diseases in the elderly. Although its cause is not fully understood, a growing body of research suggests that epigenetic mechanisms play an important role in the pathogenesis of sarcopenia. The purpose of this review is to summarize the current research progress in the epigenetics of sarcopenia, focusing on the role of DNA methylation, RNA methylation and non-coding RNA in the pathogenesis of sarcopenia. While exploring the epigenetic mechanism of sarcopenia, this study will also look into the application prospect of epigenetics in the treatment strategy of sarcopenia, which will provide new ideas and directions for the treatment of sarcopenia.展开更多
Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific rese...Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on “3332”. Methods: Three course learning modules are constructed: stage-based course learning module, systematic project practice training module and comprehensive practice training module. A practical training platform for scientific research innovation projects is built, and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines. Results: Since 2017, the students have obtained 7 national innovation and entrepreneurship training programs, 52 university-level undergraduate scientific research projects, published more than 10 academic papers, and obtained 2 patent authorization. Conclusions: The training system of scientific research innovation ability of nursing undergraduates based on “3332” is conducive to the development of scientific research innovation ability of nursing students, and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.展开更多
Objective:The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students.To explore the method and effect of training system of scientific resear...Objective:The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students.To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on“3332”.Methods:Three course learning modules are constructed:stage-based course learning module,systematic project practice training module and comprehensive practice training module.A practical training platform for scientific research innovation projects is built,and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines.Results:Since 2017,the students have obtained 7 national innovation and entrepreneurship training programs,52 university-level undergraduate scientific research projects,published more than 10 academic papers,and obtained 2 patent authorization.Conclusions:The training system of scientific research innovation ability of nursing undergraduates based on“3332”is conducive to the development of scientific research innovation ability of nursing students,and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.展开更多
直流断路器作为柔性直流电网的关键设备,对直流输配电网的安全、稳定运行有着重要的意义。针对带限流能力的直流断路器存在的造价偏高、故障清除速度慢等问题,提出了一种带自充电支路的改进型混合直流断路器(improved hybrid DC circuit...直流断路器作为柔性直流电网的关键设备,对直流输配电网的安全、稳定运行有着重要的意义。针对带限流能力的直流断路器存在的造价偏高、故障清除速度慢等问题,提出了一种带自充电支路的改进型混合直流断路器(improved hybrid DC circuit breaker with self-charging branch,SCB-IHDCCB)拓扑,设置限流电感旁路和接地旁路,优化自充电支路路线,将泄能支路与自充电支路结合使用,在保证断路器性能的前提下,减少了支路数量,大大降低了晶闸管等元器件数量,从而大大降低其造价。对所提拓扑在单端系统中的工况进行了理论推导与分析,基于所提拓扑设计了500kV的单端直流系统,在PSCAD/EMTDC中建立模型进行了仿真验证,并与其他拓扑方案的性能、元器件数量进行比较。仿真结果表明,SCB-IHDCCB的性能和造价都得到较大优化,具有良好的经济性和应用前景。展开更多
BACKGROUND Adequate bowel preparation is critical for colonoscopy screening.At present,the most widely used intestinal cleaner recommended at home and abroad is Polyethylene glycol(PEG).Intestinal cleansers can cause ...BACKGROUND Adequate bowel preparation is critical for colonoscopy screening.At present,the most widely used intestinal cleaner recommended at home and abroad is Polyethylene glycol(PEG).Intestinal cleansers can cause electrolyte disturbances and hyponatremia.However,hyponatremic encephalopathy due to hyponatremia induced by PEG solution,although rare,can lead to serious irreversible sequelae and even death.CASE SUMMARY In this report,we discuss a case of neurological dysfunction due to hyponatremia,also known as hyponatremic encephalopathy,observed in a 63-year-old woman who underwent PEG-based bowel preparation for colonoscopy.She was eventually transferred to our intensive care unit for treatment due to her Glasgow Coma Scale score of 9/15(Eye opening 2;Verbal response 1;Motor response 6)and abnormal laboratory tests.CONCLUSION Physicians should be thoroughly familiarized with the patient’s history before prescribing PEG for bowel preparation,and timely identification of patients with hyponatremic encephalopathy is essential as delayed treatment is associated with poor neurological outcomes.An intravenous infusion of 3%sodium chloride is recommended at the onset of early symptoms.The goal of treatment is to adequately treat cerebral edema while avoiding serum sodium correction beyond 15 to 20 m Eq/L within 48 h of treatment to prevent osmotic demyelination syndrome.展开更多
We present a novel scheme for dense electron acceleration driven by the laser irradiation of a near-critical-density plasma.The electron reflux effect in a transversely tailored plasma is particularly enhanced in the ...We present a novel scheme for dense electron acceleration driven by the laser irradiation of a near-critical-density plasma.The electron reflux effect in a transversely tailored plasma is particularly enhanced in the area of peak density.We observe a bubble-like distribution of re-injected electrons,which forms a strong quasistatic electromagnetic field that can accelerate electrons longitudinally while also preserving the electron transverse emittance.Simulation results demonstrate that over-dense electrons could be trapped in such an artificial bubble and accelerated to an energy of ~500 MeV.The obtained relativistic electron beam can reach a total charge of up to 0.26 nC and is well collimated with a small divergence of 17 mrad.Moreover,the wavelength of electron oscillation is noticeably reduced due to the shaking of the bubble structure in the laser field.As a result,the energy of the produced photons is substantially increased to the range.This new regime provides a path to generating high-charge electron beams and high-energy-ray sources.展开更多
Multi-photon microscopy(MPM)and coherent anti-Stokes Raman scattering(CARS)are two advanced nonlinear optical imaging techniques,which provide complementary information and have great potential in combination for noni...Multi-photon microscopy(MPM)and coherent anti-Stokes Raman scattering(CARS)are two advanced nonlinear optical imaging techniques,which provide complementary information and have great potential in combination for noninvasive in vrito biomedical applications.This paper provides a detailed discussion of the basics,development and applications of these technologies for in vrivo skin research,covering the following topics:The principle and advantage of MPM and CARS,instrumentation development for in vino applications,MPM and CARS of normal skin,application of MPM and CARS in skin cancer and disease diagnosis;application of MPM in skin disease intervention,ie.,imaging guided two-photon photothermolysis.展开更多
Tripterygium glycosides tablet(TGT),the classical commercial drug of Tripterygium wilfordii Hook.F.has been effectively used in the treatment of rheumatoid arthritis,nephrotic syndrome,leprosy,Behcet's syndrome,le...Tripterygium glycosides tablet(TGT),the classical commercial drug of Tripterygium wilfordii Hook.F.has been effectively used in the treatment of rheumatoid arthritis,nephrotic syndrome,leprosy,Behcet's syndrome,leprosy reaction and autoimmune hepatitis.However,due to its narrow and limited treatment window,TGT-induced organ toxicity(among which liver injury accounts for about 40%of clinical reports)has gained increasing attention.The present study aimed to clarify the cellular and molecular events underlying TGT-induced acute liver injury using single-cell RNA sequencing(scRNA-seq)technology.The TGT-induced acute liver injury mouse model was constructed through short-term TGT exposure and further verified by hematoxylin-eosin staining and liver function-related serum indicators,including alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase and total bilirubin.Using the mouse model,we identified 15 specific subtypes of cells in the liver tissue,including endothelial cells,hepatocytes,cholangiocytes,and hepatic stellate cells.Further analysis indicated that TGT caused a significant inflammatory response in liver endothelial cells at different spatial locations;led to marked inflammatory response,apoptosis and fatty acid metabolism dysfunction in hepatocytes;activated hepatic stellate cells;brought about the activation,inflammation,and phagocytosis of liver capsular macrophages cells;resulted in immune dysfunction of liver lymphocytes;disturbed the intercellular crosstalk in liver microenvironment by regulating various signaling pathways.Thus,these findings elaborate the mechanism underlying TGT-induced acute liver injury,provide new insights into the safe and rational applications in the clinic,and complement the identification of new biomarkers and therapeutic targets for liver protection.展开更多
Realizing single light solitons that are stable in high dimensions is a long-standing goal in research of nonlinear optical physics.Here,we address a scheme to generate stable two-dimensional solitons in a cold Rydber...Realizing single light solitons that are stable in high dimensions is a long-standing goal in research of nonlinear optical physics.Here,we address a scheme to generate stable two-dimensional solitons in a cold Rydberg atomic system with a parity-time(PT) symmetric moiré optical lattice.We uncover the formation,properties,and their dynamics of fundamental and two-pole gap solitons as well as vortical ones.The PT symmetry,lattice strength,and the degrees of local and nonlocal nonlinearity are tunable and can be used to control solitons.The stability regions of these solitons are evaluated in two numerical ways:linear-stability analysis and time evolutions with perturbations.Our results provide an insightful understanding of solitons physics in combined versatile platforms of PT-symmetric systems and Rydberg–Rydberg interaction in cold gases.展开更多
文摘Objectives: In this study, bibliometric approach was used to explore the literature in the field of research related to Diabetic Myasthenia Gravis in order to identify the current research progress in Diabetic Myasthenia Gravis related research and to help the researchers to predict the future hotspots in the field of research and to provide reference for the research. Methods: Literature related to diabetic sarcopenia published from 1993 to 2023 since the inception of the repository was extracted from the Web of Science Core Collection (WoSCC) and bibliometric analyses were performed. We have analysed the published literature of the last approximately almost 30 years, as well as publication and citation analyses from different countries, institutions, journals and authors. For keywords, we performed co-occurrence, clustering, timeline view and citation burst analysis. Results: On the basis of 1564 publications, we found a continuous increase in the number of publications and citations, especially in the last six years. The United States is the most representative country, and Seoul National University (SNU) is the most representative institution. The most popular journal in the field is Diabetes Care;Fukui, Michiaki is the most prolific author, leading many studies related to diabetic sarcopenia. The most frequently cited reference was a revised European consensus on the definition and diagnosis of sarcopenia;the most cited keywords were related to physiological factors of diabetes, sarcopenia and related conditions, such as “insulin resistance”, “skeletal muscle”, “body composition”, “risk” and “prevalence”. Conclusion: With more and more studies on the relationship between diabetic sarcopenia, this study presents the current status and trend of research related to diabetic sarcopenia over the past nearly 30 years through the visualization software CiteSpace. It can help researchers identify potential collaborators and partner institutions, hotspots and research frontiers in the field of diabetic sarcopenia. However, our work is only based on the English language literature in the WoSCC database, and for future studies, we recommend that researchers explore the literature from multiple databases to enhance the scope of their research.
文摘Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response inβcells remains unclear.Methods:To investigate this,we induced early-onset T1D in non-obese diabetic mice using streptozotocin.Subsequently,T1D mice were randomly assigned to receive either MSCs or phosphate-buffered saline.We observed the in vivo homing of MSCs and assessed their effectiveness by analyzing blood glucose levels,body weight,histopathology,pancreatic protein expression,and serum levels of cytokines,proinsulin,and C-peptide.Results:Infused MSCs were found in the lungs,liver,spleen,and pancreas of T1D mice.They exhibited various effects,including reducing blood glucose levels,regulating immunity,inhibiting inflammation,increasingβ-cell areas,and reducing the expression of key proteins in the unfolded protein response pathway.Fasting serum proinsulin and C-peptide levels were significantly higher in the MSCs treatment group than in the T1D model group.However,there was no significant difference in the biomarker ofβ-cell endoplasmic reticulum stress,the ratio of fasting serum proinsulin to C-peptide,between the two groups.Conclusion:Ourfindings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress inβcells directly but modulates the unfolded protein response pathway to preserveβ-cell mass and function in T1D mice.
文摘在自动驾驶场景下的3D目标检测任务中,探索毫米波雷达数据作为RGB图像输入的补充正成为多模态融合的新兴趋势。然而,现有的毫米波雷达-相机融合方法高度依赖于相机的一阶段检测结果,导致整体性能不够理想。本文提供了一种不依赖于相机检测结果的鸟瞰图下双向融合方法(BEV-radar)。对于来自不同域的两个模态的特征,BEV-radar设计了一个双向的基于注意力的融合策略。具体地,以基于BEV的3D目标检测方法为基础,我们的方法使用双向转换器嵌入来自两种模态的信息,并根据后续的卷积块强制执行局部空间关系。嵌入特征后,BEV特征在3D对象预测头中解码。我们在nu Scenes数据集上评估了我们的方法,实现了48.2 m AP和57.6 NDS。结果显示,与仅使用相机的基础模型相比,不仅在精度上有所提升,特别地,速度预测误差项有了相当大的改进。代码开源于https://github.com/Etah0409/BEV-Radar。
基金supported by the Science and Technology Project of North China Electric Power Research Institute,which is“Research on Key Technologies for Power Quality Evaluation and Improvement of New Distribution Network Based on Collaborative Interaction of Source-Network-Load-Storage”(KJZ2022016).
文摘The couple between the power network and the transportation network(TN)is deepening gradually with the increasing penetration rate of electric vehicles(EV),which also poses a great challenge to the traditional voltage control scheme.In this paper,we propose a coordinated voltage control strategy for the active distribution networks considering multiple types of EV.In the first stage,the action of on-load tap changer and capacitor banks,etc.,are determined by optimal power flow calculation,and the node electricity price is also determined based on dynamic time-of-use tariff mechanism.In the second stage,multiple operating scenarios of multiple types of EVs such as cabs,private cars and buses are considered,and the scheduling results of each EV are solved by building an optimization model based on constraints such as queuing theory,Floyd-Warshall algorithm and traffic flow information.In the third stage,the output power of photovoltaic and energy storage systems is fine-tuned in the normal control mode.The charging power of EVs is also regulated in the emergency control mode to reduce the voltage deviation,and the amount of regulation is calculated based on the fair voltage control mode of EVs.Finally,we test the modified IEEE 33-bus distribution system coupled with the 24-bus Beijing TN.The simulation results show that the proposed scheme can mitigate voltage violations well.
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the role of TMEM100 in esophageal cancer(EC).AIM To investigate epigenetic regulation of TMEM100 expression in ESCC and the effect of TMEM100 on ESCC proliferation and invasion.METHODS Firstly,we found the expression of TMEM100 in EC through The Cancer Genome Atlas database.The correlation between TMEM100 gene expression and the survival of patients with EC was further confirmed through Kaplan-Meier analysis.We then added the demethylating agent 5-AZA to ESCC cell lines to explore the regulation of TMEM100 expression by epigenetic modification.To observe the effect of TMEM100 expression on tumor proliferation and invasion by overexpressing TMEM100.Finally,we performed gene set enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes Orthology-Based Annotation System database to look for pathways that might be affected by TMEM100 and verified the effect of TMEM100 expression on the mitogen-activated protein kinases(MAPK)pathway.RESULTS In the present study,by bioinformatic analysis we found that TMEM100 was lowly expressed in EC patients compared to normal subjects.Kaplan-meier survival analysis showed that low expression of TMEM100 was associated with poor prognosis in patients with EC.Then,we found that the demethylating agent 5-AZA resulted in increased expression of TMEM100 in ESCC cells[quantitative real-time PCR(qRT-PCR)and western blotting].Subsequently,we confirmed that overexpression of TMEM100 leads to its increased expression in ESCC cells(qRT-PCR and western blotting).Overexpression of TMEM100 also inhibited proliferation,invasion and migration of ESCC cells(cell counting kit-8 and clone formation assays).Next,by enrichment analysis,we found that the gene set was significantly enriched in the MAPK signaling pathway.The involvement of TMEM100 in the regulation of MAPK signaling pathway in ESCC cell was subsequently verified by western blotting.CONCLUSION TMEM100 is a suppressor gene in ESCC,and its low expression may lead to aberrant activation of the MAPK pathway.Promoter methylation may play a key role in regulating TMEM100 expression.
文摘In recent years, sarcopenia, as a progressive muscular atrophy and weakness, has become one of the common diseases in the elderly. Although its cause is not fully understood, a growing body of research suggests that epigenetic mechanisms play an important role in the pathogenesis of sarcopenia. The purpose of this review is to summarize the current research progress in the epigenetics of sarcopenia, focusing on the role of DNA methylation, RNA methylation and non-coding RNA in the pathogenesis of sarcopenia. While exploring the epigenetic mechanism of sarcopenia, this study will also look into the application prospect of epigenetics in the treatment strategy of sarcopenia, which will provide new ideas and directions for the treatment of sarcopenia.
文摘Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on “3332”. Methods: Three course learning modules are constructed: stage-based course learning module, systematic project practice training module and comprehensive practice training module. A practical training platform for scientific research innovation projects is built, and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines. Results: Since 2017, the students have obtained 7 national innovation and entrepreneurship training programs, 52 university-level undergraduate scientific research projects, published more than 10 academic papers, and obtained 2 patent authorization. Conclusions: The training system of scientific research innovation ability of nursing undergraduates based on “3332” is conducive to the development of scientific research innovation ability of nursing students, and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.
文摘Objective:The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students.To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on“3332”.Methods:Three course learning modules are constructed:stage-based course learning module,systematic project practice training module and comprehensive practice training module.A practical training platform for scientific research innovation projects is built,and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines.Results:Since 2017,the students have obtained 7 national innovation and entrepreneurship training programs,52 university-level undergraduate scientific research projects,published more than 10 academic papers,and obtained 2 patent authorization.Conclusions:The training system of scientific research innovation ability of nursing undergraduates based on“3332”is conducive to the development of scientific research innovation ability of nursing students,and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.
文摘直流断路器作为柔性直流电网的关键设备,对直流输配电网的安全、稳定运行有着重要的意义。针对带限流能力的直流断路器存在的造价偏高、故障清除速度慢等问题,提出了一种带自充电支路的改进型混合直流断路器(improved hybrid DC circuit breaker with self-charging branch,SCB-IHDCCB)拓扑,设置限流电感旁路和接地旁路,优化自充电支路路线,将泄能支路与自充电支路结合使用,在保证断路器性能的前提下,减少了支路数量,大大降低了晶闸管等元器件数量,从而大大降低其造价。对所提拓扑在单端系统中的工况进行了理论推导与分析,基于所提拓扑设计了500kV的单端直流系统,在PSCAD/EMTDC中建立模型进行了仿真验证,并与其他拓扑方案的性能、元器件数量进行比较。仿真结果表明,SCB-IHDCCB的性能和造价都得到较大优化,具有良好的经济性和应用前景。
文摘BACKGROUND Adequate bowel preparation is critical for colonoscopy screening.At present,the most widely used intestinal cleaner recommended at home and abroad is Polyethylene glycol(PEG).Intestinal cleansers can cause electrolyte disturbances and hyponatremia.However,hyponatremic encephalopathy due to hyponatremia induced by PEG solution,although rare,can lead to serious irreversible sequelae and even death.CASE SUMMARY In this report,we discuss a case of neurological dysfunction due to hyponatremia,also known as hyponatremic encephalopathy,observed in a 63-year-old woman who underwent PEG-based bowel preparation for colonoscopy.She was eventually transferred to our intensive care unit for treatment due to her Glasgow Coma Scale score of 9/15(Eye opening 2;Verbal response 1;Motor response 6)and abnormal laboratory tests.CONCLUSION Physicians should be thoroughly familiarized with the patient’s history before prescribing PEG for bowel preparation,and timely identification of patients with hyponatremic encephalopathy is essential as delayed treatment is associated with poor neurological outcomes.An intravenous infusion of 3%sodium chloride is recommended at the onset of early symptoms.The goal of treatment is to adequately treat cerebral edema while avoiding serum sodium correction beyond 15 to 20 m Eq/L within 48 h of treatment to prevent osmotic demyelination syndrome.
基金supported by the China Postdoctoral Science Foundation(Grant No.2021M692204)the National Natural Science Foundation of China(Grant No.11805278)+3 种基金the Fundamental Research Program of Shenzhen(Grant No.SZWD2021007)the Science and Technology on Plasma Physics Laboratorythe Guangdong Basic and Applied Basic Research Foundation(Grant No.2022A1515010326)the Shenzhen Technology University.
文摘We present a novel scheme for dense electron acceleration driven by the laser irradiation of a near-critical-density plasma.The electron reflux effect in a transversely tailored plasma is particularly enhanced in the area of peak density.We observe a bubble-like distribution of re-injected electrons,which forms a strong quasistatic electromagnetic field that can accelerate electrons longitudinally while also preserving the electron transverse emittance.Simulation results demonstrate that over-dense electrons could be trapped in such an artificial bubble and accelerated to an energy of ~500 MeV.The obtained relativistic electron beam can reach a total charge of up to 0.26 nC and is well collimated with a small divergence of 17 mrad.Moreover,the wavelength of electron oscillation is noticeably reduced due to the shaking of the bubble structure in the laser field.As a result,the energy of the produced photons is substantially increased to the range.This new regime provides a path to generating high-charge electron beams and high-energy-ray sources.
文摘Multi-photon microscopy(MPM)and coherent anti-Stokes Raman scattering(CARS)are two advanced nonlinear optical imaging techniques,which provide complementary information and have great potential in combination for noninvasive in vrito biomedical applications.This paper provides a detailed discussion of the basics,development and applications of these technologies for in vrivo skin research,covering the following topics:The principle and advantage of MPM and CARS,instrumentation development for in vino applications,MPM and CARS of normal skin,application of MPM and CARS in skin cancer and disease diagnosis;application of MPM in skin disease intervention,ie.,imaging guided two-photon photothermolysis.
基金supported by the National Key Research and Development Program of China(Grant Nos.:2020YFA0908000,2022YFC2303600)the Establishment of Sino-Austria“Belt and Road”Joint Laboratory on Traditional Chinese Medicine for Severe Infectious Diseases and Joint Research(Grant No.:2020YFE0205100)+13 种基金the National Natural Science Foundation of China(Grant Nos.:82104480,82004248,82141001,82274182,82074098,82173914)the Fundamental Research Funds for the Central public welfare research institutes(Grant Nos.:ZZ14-YQ-055,ZZ14-YQ-059,ZZ14-YQ-060,ZXKT19018,ZXKT19021,ZXKT19022,ZZ14-YQ-050,ZZ14-YQ-051,ZZ14-YQ-052,ZZ14-FL-002,ZZ14-ND-010,ZZ15-ND-10,ZZ16-ND-10-19)the Beijing Municipal Natural Science Foundation(Grant No.:7214287)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(Grant No.:ZYYCXTD-C-202002)the Young Elite Scientists Sponsorship Program by CACM(Grant No.:2021QNRC2B29)the CACMS Innovation Fund(Grant Nos.:CI2021A05101,CI2021A05104)the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(Grant No.:CI2021B014)the Science and Technology Foundation of Shenzhen(Grant No.:JCYJ20210324115800001)the Science and Technology Foundation of Shenzhen(Shenzhen Clinical Medical Research Center for Geriatric Diseases)Shenzhen Governmental Sustainable Development Fund(Grant No.:KCXFZ20201221173612034)Shenzhen key Laboratory of Kidney Diseases(Grant No.:ZDSYS201504301616234)Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties(Grant No.:SZGSP001)the Distinguished Expert Project of Sichuan Province Tianfu Scholar(Grant No.:CW202002)the State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Open Fund(Grant No.:SKL2020Z0302).
文摘Tripterygium glycosides tablet(TGT),the classical commercial drug of Tripterygium wilfordii Hook.F.has been effectively used in the treatment of rheumatoid arthritis,nephrotic syndrome,leprosy,Behcet's syndrome,leprosy reaction and autoimmune hepatitis.However,due to its narrow and limited treatment window,TGT-induced organ toxicity(among which liver injury accounts for about 40%of clinical reports)has gained increasing attention.The present study aimed to clarify the cellular and molecular events underlying TGT-induced acute liver injury using single-cell RNA sequencing(scRNA-seq)technology.The TGT-induced acute liver injury mouse model was constructed through short-term TGT exposure and further verified by hematoxylin-eosin staining and liver function-related serum indicators,including alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase and total bilirubin.Using the mouse model,we identified 15 specific subtypes of cells in the liver tissue,including endothelial cells,hepatocytes,cholangiocytes,and hepatic stellate cells.Further analysis indicated that TGT caused a significant inflammatory response in liver endothelial cells at different spatial locations;led to marked inflammatory response,apoptosis and fatty acid metabolism dysfunction in hepatocytes;activated hepatic stellate cells;brought about the activation,inflammation,and phagocytosis of liver capsular macrophages cells;resulted in immune dysfunction of liver lymphocytes;disturbed the intercellular crosstalk in liver microenvironment by regulating various signaling pathways.Thus,these findings elaborate the mechanism underlying TGT-induced acute liver injury,provide new insights into the safe and rational applications in the clinic,and complement the identification of new biomarkers and therapeutic targets for liver protection.
基金supported by the National Natural Science Foundation of China(Grant Nos.62275075,11975172,and 12261131495)the Shanghai Outstanding Academic Leaders Plan (Grant No.20XD1402000)the Training Program of Innovation and Entrepreneurship for Undergraduates of Hubei Province (Grant No.S202210927036)。
文摘Realizing single light solitons that are stable in high dimensions is a long-standing goal in research of nonlinear optical physics.Here,we address a scheme to generate stable two-dimensional solitons in a cold Rydberg atomic system with a parity-time(PT) symmetric moiré optical lattice.We uncover the formation,properties,and their dynamics of fundamental and two-pole gap solitons as well as vortical ones.The PT symmetry,lattice strength,and the degrees of local and nonlocal nonlinearity are tunable and can be used to control solitons.The stability regions of these solitons are evaluated in two numerical ways:linear-stability analysis and time evolutions with perturbations.Our results provide an insightful understanding of solitons physics in combined versatile platforms of PT-symmetric systems and Rydberg–Rydberg interaction in cold gases.