This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients an...This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients and 4 controls were subjected to tRF and tiRNA polymerase chain reaction(PCR)Array analysis.Then quantitative PCR(qPCR)validation was performed on all the samples.Bioinformatics analyses were conducted to explore their functions.Results:We found 115 tsRNAs that significantly differed between the two groups.3′tiR-080-ProTGG(mt)was selected for further qPCR validation in all participants,and it was significantly decreased in diabetes patients compared with controls.Bioinformatics analysis indicated that 3′tiR-080-ProTGG(mt)may play regulatory roles via the cyclic adenosine monophosphate(cAMP)signaling pathway in the pathogenesis and progression of diabetes.Conclusion:Hence,we report that circulating tsRNAs are dysregulated that could be involved in the pathogenesis of diabetes.展开更多
基金funded by the Project of the Shanghai Municipal Health Commission(Grant No.20194Y0268)Project of Science and Technology Commission of Shanghai Municipality(Grant No.20Z11900400).
文摘This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients and 4 controls were subjected to tRF and tiRNA polymerase chain reaction(PCR)Array analysis.Then quantitative PCR(qPCR)validation was performed on all the samples.Bioinformatics analyses were conducted to explore their functions.Results:We found 115 tsRNAs that significantly differed between the two groups.3′tiR-080-ProTGG(mt)was selected for further qPCR validation in all participants,and it was significantly decreased in diabetes patients compared with controls.Bioinformatics analysis indicated that 3′tiR-080-ProTGG(mt)may play regulatory roles via the cyclic adenosine monophosphate(cAMP)signaling pathway in the pathogenesis and progression of diabetes.Conclusion:Hence,we report that circulating tsRNAs are dysregulated that could be involved in the pathogenesis of diabetes.
