To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by ...To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by using the approach of plant proteomics, and those proteins were separated with two-dimensional electrophoresis （2-DE） and then analyzed with an imagemaster 2D Elite 5.0. The results showed that the 41 protein spots were detected differential expression, of which 17 new protein spots appeared after the 3-leaf stage, including 9 special protein spots, which were only detected at the 3-leaf stage. Thirteen protein spots increased first and then decreased in expression abundance gradually and finally even disappeared. For the other 11 protein spots, 3 protein spots decreased, but 6 protein spots were opposite in expression abundance, however, 2 protein spots expressed in an irregular pattern after the 2-leaf stage. Of the 41 differential leaf proteins, 15 protein spots were identified by ESI-Q MS/MS and categorized into 4 groups of functions. The results indicated that proteins were the carriers of the functions in cells, but were significantly influenced by the changes in cell function or intercellular environment; hence, the reason that caused the proteomic changes as mentioned earlier might be related to the occurrence of tillers at the rice seedling stage after the 3-leaf stage.展开更多
基金the National Natural Science Foundation of China (30600385)the National Key Technology R&D Program of China (2004NZ0104)
文摘To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by using the approach of plant proteomics, and those proteins were separated with two-dimensional electrophoresis （2-DE） and then analyzed with an imagemaster 2D Elite 5.0. The results showed that the 41 protein spots were detected differential expression, of which 17 new protein spots appeared after the 3-leaf stage, including 9 special protein spots, which were only detected at the 3-leaf stage. Thirteen protein spots increased first and then decreased in expression abundance gradually and finally even disappeared. For the other 11 protein spots, 3 protein spots decreased, but 6 protein spots were opposite in expression abundance, however, 2 protein spots expressed in an irregular pattern after the 2-leaf stage. Of the 41 differential leaf proteins, 15 protein spots were identified by ESI-Q MS/MS and categorized into 4 groups of functions. The results indicated that proteins were the carriers of the functions in cells, but were significantly influenced by the changes in cell function or intercellular environment; hence, the reason that caused the proteomic changes as mentioned earlier might be related to the occurrence of tillers at the rice seedling stage after the 3-leaf stage.
