Dissolved organic matter (DOM) is one of the most interesting and difficult problems in recent years due to its important functions in the ecological and environmental system and the complexity of its chemical composi...Dissolved organic matter (DOM) is one of the most interesting and difficult problems in recent years due to its important functions in the ecological and environmental system and the complexity of its chemical composition and structure. It is well ac- cepted that fluorescence characteristics and mo- lecular weight distribution are two important pa- rameters in the DOM characterization. However, the relationship between them is still unknown. In this study, fluorescence and molecular weight distribution of DOM in Lake Hongfeng, Lake Baihua and their rivers, and their relationship were investigated using the combination of fluorescence spectroscopy and high-performance size exclusion chromatography (HPSEC) with on-line UV absorbance and fluores- cence detectors. The results show that there were two obvious humic-like fluorescence peaks (Peaks A and B) in DOM from lake water. But there was an- other obvious protein-like fluorescence peak (Peak C) in DOM from river water. The humic-like fluorescence material consisted of DOM fraction with smaller mo- lecular weight, ranging from 1.0 to 3.0 kDa, while the protein-like fluorescence material mainly consisted of DOM fraction with MW larger than 2.0 kDa. The cal- culation of MW using HPSEC was related to the UV absorbance wavelength chosen.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.40525011,40403011)973 Projects(Grant No.2002CB412300)the National Knowledge Innovation Program of the Chinese Academy of Sciences(Grant No.KZCX2-W1).
文摘Dissolved organic matter (DOM) is one of the most interesting and difficult problems in recent years due to its important functions in the ecological and environmental system and the complexity of its chemical composition and structure. It is well ac- cepted that fluorescence characteristics and mo- lecular weight distribution are two important pa- rameters in the DOM characterization. However, the relationship between them is still unknown. In this study, fluorescence and molecular weight distribution of DOM in Lake Hongfeng, Lake Baihua and their rivers, and their relationship were investigated using the combination of fluorescence spectroscopy and high-performance size exclusion chromatography (HPSEC) with on-line UV absorbance and fluores- cence detectors. The results show that there were two obvious humic-like fluorescence peaks (Peaks A and B) in DOM from lake water. But there was an- other obvious protein-like fluorescence peak (Peak C) in DOM from river water. The humic-like fluorescence material consisted of DOM fraction with smaller mo- lecular weight, ranging from 1.0 to 3.0 kDa, while the protein-like fluorescence material mainly consisted of DOM fraction with MW larger than 2.0 kDa. The cal- culation of MW using HPSEC was related to the UV absorbance wavelength chosen.
