We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis metho...We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis method .The resonance with JXPXC=1-+ can e distingguished from other resonances by means of these moments exceptfor some rather special cases.The suggestion that the search for the hybrid with IG(JPC)=1-(1-+) can be performed in the decay channl J/ψ→ρωππat upgraded BEPC/BES is presented.展开更多
AIM: To assess the variability of adhesin gene hpaA between different Helicobacter pylori ( H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were c...AIM: To assess the variability of adhesin gene hpaA between different Helicobacter pylori ( H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were chosento amplify the 710-bp segments of gene hpaA. These strains were NCTC11637, SS1; Chongqing clinical isolates CCS9801, CCS9802, CCS9803, CCS9806, CCS9809,CCS9810, CCS9813, which were gained from patients of gastritis; Mongolia gerbil adapted H pylori strains (abbreviation MG), which were gained from the following steps: gastric mucosal specimens of Mongolia gerbils infected by clinical isolate CCS9803 were cultured and detected, the positive H pylori strains were named as the first generation of Mongolia gerbil adapted H pylori strains(abbreviation MG1) and then were subcultured with healthy Mongolia gerbil to generate MG2, in turn to gain the ninth generation (abbreviation MG9). All hpaA segments, obtained from 12 different H pylori strains,were digested by HhaⅠ and HaeⅢ individually and analyzed by agarose gel electrophoresis. RESULTS: In all 12 strains, the 710-bp PCR products were successfully amplified and products were cloned to pMD18T vector respectively, then the recombinant plasmids were digested simultaneously with NcoⅠ and XhoⅠ to recover the small fragments. The objective fragments from 12 different H pylori strains digested with Hae Ⅲ could be seen as 4 types of bands and 5 types with Hha Ⅰ. According to the hpaA RFLP patterns, the 12 H pylori strains could be divided into 5 groups: group Ⅰ, NCTC11637 and SS1; group Ⅱ, CCS9809, which RFLP type digested with HaeⅢ wasthe same as strains of group Ⅰ, but HhaⅠ RFLP showeddifference compared with the other groups; group Ⅲ,CCS9810; group Ⅳ, CCS9803; group Ⅴ: CCS9801,CCS9802, CCS9806, CCS9813, MG1, MG3 and MG9. The sequence data of 12 hpaA segments were analyzed by DNAsis software and it was observed that: (1) The homologies of base pair and amino acid sequence between strains NCTC11637, SS1, CCS9809 were 99.6% and 98.9%, respectively; (2) The homology of base pair and amino acid sequence between CCS9803 and CCS9810 was 97.7% and 99.1%; (3) That of the rest strains, CCS9801, CCS9802, CCS9806, CCS9813, MG1, MG3, MG9 reached 99.4% and98.4%; (4) The base pair homologies between all hpaAfragments of different sources were higher than 94.6%,therefore the correspondence of deduced amino acid sequence was higher than 96.8% between each other. CONCLUSION: The gene hpaA from different H pylori strains revealed variation, and this might provide an effectivemethod for molecular epidemiological survey of H pylori.展开更多
The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of e...The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway. Key words oxidized low density lipoproteins - macrophage - matrix metalloproteinases CLC number Q 556+.9 Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis.展开更多
1.Ma?da Calendar and Akiti Calendar from ? 45 to 48The dating of the texts from the reign of ?ulgi is quite complex work since from ? 45 onwards,the first month of a year started from the Akiti month,which was the six...1.Ma?da Calendar and Akiti Calendar from ? 45 to 48The dating of the texts from the reign of ?ulgi is quite complex work since from ? 45 onwards,the first month of a year started from the Akiti month,which was the sixth month of the normal Ma?da Calendar used in Puzri?-Dagan.The Puzri?-Dagan calendar followed the Ur Calendar,but during the period of ?ulgi and Amar-Sin,its second month,iti-ma?-dà～gu7,was changed to become the first month,so that the month iti-?e-kin-kud could be synchronized with the month of the same name at Nippur,the 12th month of both calendars.We have also found some texts which use the month of Akiti as the first month and the month of itiezem-Ninazu as the last month,which it is in the Akiti Calendar.We distinguish the Akiti Calendar as ? 44’viii’→48’vii’,which is equal to ? 45 i→48 xii in the Ma?da Calendar,for four years.展开更多
Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based ...Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based on rough set theory. First, the relation of the newinstances with the original rule set is discussed. Then the change law of attribute reduction andvalue reduction are studied when a new instance is added. Follow, a new incremental learningalgorithm for decision tables is presented within the framework of rough set. Finally, the newalgorithm and the classical algorithm are analyzed and compared by theory and experiments.展开更多
文摘We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis method .The resonance with JXPXC=1-+ can e distingguished from other resonances by means of these moments exceptfor some rather special cases.The suggestion that the search for the hybrid with IG(JPC)=1-(1-+) can be performed in the decay channl J/ψ→ρωππat upgraded BEPC/BES is presented.
基金Supported by the Major Projects of the National High Technology R&D (863) Program of China, No. 2001AA215161
文摘AIM: To assess the variability of adhesin gene hpaA between different Helicobacter pylori ( H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were chosento amplify the 710-bp segments of gene hpaA. These strains were NCTC11637, SS1; Chongqing clinical isolates CCS9801, CCS9802, CCS9803, CCS9806, CCS9809,CCS9810, CCS9813, which were gained from patients of gastritis; Mongolia gerbil adapted H pylori strains (abbreviation MG), which were gained from the following steps: gastric mucosal specimens of Mongolia gerbils infected by clinical isolate CCS9803 were cultured and detected, the positive H pylori strains were named as the first generation of Mongolia gerbil adapted H pylori strains(abbreviation MG1) and then were subcultured with healthy Mongolia gerbil to generate MG2, in turn to gain the ninth generation (abbreviation MG9). All hpaA segments, obtained from 12 different H pylori strains,were digested by HhaⅠ and HaeⅢ individually and analyzed by agarose gel electrophoresis. RESULTS: In all 12 strains, the 710-bp PCR products were successfully amplified and products were cloned to pMD18T vector respectively, then the recombinant plasmids were digested simultaneously with NcoⅠ and XhoⅠ to recover the small fragments. The objective fragments from 12 different H pylori strains digested with Hae Ⅲ could be seen as 4 types of bands and 5 types with Hha Ⅰ. According to the hpaA RFLP patterns, the 12 H pylori strains could be divided into 5 groups: group Ⅰ, NCTC11637 and SS1; group Ⅱ, CCS9809, which RFLP type digested with HaeⅢ wasthe same as strains of group Ⅰ, but HhaⅠ RFLP showeddifference compared with the other groups; group Ⅲ,CCS9810; group Ⅳ, CCS9803; group Ⅴ: CCS9801,CCS9802, CCS9806, CCS9813, MG1, MG3 and MG9. The sequence data of 12 hpaA segments were analyzed by DNAsis software and it was observed that: (1) The homologies of base pair and amino acid sequence between strains NCTC11637, SS1, CCS9809 were 99.6% and 98.9%, respectively; (2) The homology of base pair and amino acid sequence between CCS9803 and CCS9810 was 97.7% and 99.1%; (3) That of the rest strains, CCS9801, CCS9802, CCS9806, CCS9813, MG1, MG3, MG9 reached 99.4% and98.4%; (4) The base pair homologies between all hpaAfragments of different sources were higher than 94.6%,therefore the correspondence of deduced amino acid sequence was higher than 96.8% between each other. CONCLUSION: The gene hpaA from different H pylori strains revealed variation, and this might provide an effectivemethod for molecular epidemiological survey of H pylori.
文摘The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway. Key words oxidized low density lipoproteins - macrophage - matrix metalloproteinases CLC number Q 556+.9 Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis.
基金Supported by the High Technology Research and Development Programme of China （No. 2003AA404060） and the National Natural Science Foundation of China （No.60405008）.
基金supported by the Noble Group Fundthe W.F.Albright Institute of Archaeological ResearchJerusalem
文摘1.Ma?da Calendar and Akiti Calendar from ? 45 to 48The dating of the texts from the reign of ?ulgi is quite complex work since from ? 45 onwards,the first month of a year started from the Akiti month,which was the sixth month of the normal Ma?da Calendar used in Puzri?-Dagan.The Puzri?-Dagan calendar followed the Ur Calendar,but during the period of ?ulgi and Amar-Sin,its second month,iti-ma?-dà～gu7,was changed to become the first month,so that the month iti-?e-kin-kud could be synchronized with the month of the same name at Nippur,the 12th month of both calendars.We have also found some texts which use the month of Akiti as the first month and the month of itiezem-Ninazu as the last month,which it is in the Akiti Calendar.We distinguish the Akiti Calendar as ? 44’viii’→48’vii’,which is equal to ? 45 i→48 xii in the Ma?da Calendar,for four years.
基金This work is supported by National Science Foundation of China (No.60373111).
文摘Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based on rough set theory. First, the relation of the newinstances with the original rule set is discussed. Then the change law of attribute reduction andvalue reduction are studied when a new instance is added. Follow, a new incremental learningalgorithm for decision tables is presented within the framework of rough set. Finally, the newalgorithm and the classical algorithm are analyzed and compared by theory and experiments.
