The compound KdV-Burgers equation and combined KdV-mKdV equation are real physical models concerning many branches in physics.In this paper,applying the improved trigonometric function method to these equations,rich e...The compound KdV-Burgers equation and combined KdV-mKdV equation are real physical models concerning many branches in physics.In this paper,applying the improved trigonometric function method to these equations,rich explicit and exact travelling wave solutions,which contain solitary-wave solutions,periodic solutions,and combined formal solitary-wave solutions,are obtained.展开更多
A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and mon...A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin (HA) genes of the pandemic H1N1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic H1N1/2009 virus infection.展开更多
In this letter, a class of reaction-diffusion equations, which arise in chemical reaction or ecology and other fields of physics, are investigated. A more general analytical solution of the equation is obtained by usi...In this letter, a class of reaction-diffusion equations, which arise in chemical reaction or ecology and other fields of physics, are investigated. A more general analytical solution of the equation is obtained by using the first integral method.展开更多
Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specifici...Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specificity of anti-Id antibody was established by ELISA.The 84kD protein inhibited the binding of anti-Id to YWK-I mAb,but failed to repress antibody against normal mouse Ig binding to YWK-I mAb.In competitive inhibition assay,84kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner.Crude sperm extract showed a lower competitive ability.No effect was found with the irrelevant 36kD sperm protein.The antisera from the Balb/C micr immunized with AId contained Ab3 that reacted with 84kD sperm protein.The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sperm agglutination.These results indicate that anti-Id which may mimic an epitope of the 84kD protein could be exploited as an antigen to raise antibodies against sperm protein.展开更多
Attention has been paid to the potential application of the network concept for producing contraceptive vaccine. The existence of a special class of antibodies capable of
The key techniques of modular design of heavy duty NC mathine tools are described. Amodule definition modelfor modular design and manufacturing of heavy duty NC machine tools isbulit and the essential composition of t...The key techniques of modular design of heavy duty NC mathine tools are described. Amodule definition modelfor modular design and manufacturing of heavy duty NC machine tools isbulit and the essential composition of the module definition model (MDM) is discussed in detail. Itis composed of two models: the part definition model (PDM) and the module assembly model(MAM). The PDM and MAM are built and their structures are given. Using object-oriented know-ledge representation and based on these models, an intelligent support system of modular design forheavy duty NC machine tools is developed and implemented This system has been applied to thepractical use of Wuhan Heavy Duty Machine Tool Works展开更多
The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and product...The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and productive traits such as growth rate and carcass composition. As yet system- atical research on SLA expression profile is not reported. In order to illustrate SLA expression comprehensively and deepen our understanding of its function, we outlined the expression profile of SLA in 51 tissues of Landrace by ana- lyzing a large amount of ESTs produced by “Sino-Danish Porcine Genome Project”. In addition, we also compared the expression profile of SLA in several tissues from different development stages and from another breed (Erhualian). The result shows: (i) classical SLA genes are highly expressed in immune tissues and middle part of intestine; (ii) although SLA-3 is an SLA Ia gene, its expression abundance and pat- tern are quite different from those of the other two SLA Ia genes. The same phenomenon is seen in HLA-C expression, suggesting that the two genes may function similarly and undergo convergent evolution; (iii) except in jejunum, the antigen presenting genes are more highly expressed in breed Erhualian than in Landrace. The difference might associate with the higher resistance to bad conditions (including pathogens) of Erhualian and higher growth rates of Land- race.展开更多
A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by...A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.展开更多
文摘The compound KdV-Burgers equation and combined KdV-mKdV equation are real physical models concerning many branches in physics.In this paper,applying the improved trigonometric function method to these equations,rich explicit and exact travelling wave solutions,which contain solitary-wave solutions,periodic solutions,and combined formal solitary-wave solutions,are obtained.
基金supported by grants from National Basic Research Program of China (No.2011CB504800)National Natural Science Foundation of China (No. 31100128 and 81030031)+3 种基金National Mega Project on Major Drug Development (2009ZX09103-678)National Small Business Innovation and Research (SBIR) Program of Chinathe Technology R & D Program of Jiangsu Province, China (BG20077035 and BG2008662)NIH (RO1-AI041927,RO1-AI050468, RO1-DE014145, and RO1-DE014842)
文摘A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin (HA) genes of the pandemic H1N1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic H1N1/2009 virus infection.
文摘In this letter, a class of reaction-diffusion equations, which arise in chemical reaction or ecology and other fields of physics, are investigated. A more general analytical solution of the equation is obtained by using the first integral method.
文摘Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specificity of anti-Id antibody was established by ELISA.The 84kD protein inhibited the binding of anti-Id to YWK-I mAb,but failed to repress antibody against normal mouse Ig binding to YWK-I mAb.In competitive inhibition assay,84kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner.Crude sperm extract showed a lower competitive ability.No effect was found with the irrelevant 36kD sperm protein.The antisera from the Balb/C micr immunized with AId contained Ab3 that reacted with 84kD sperm protein.The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sperm agglutination.These results indicate that anti-Id which may mimic an epitope of the 84kD protein could be exploited as an antigen to raise antibodies against sperm protein.
文摘Attention has been paid to the potential application of the network concept for producing contraceptive vaccine. The existence of a special class of antibodies capable of
文摘The key techniques of modular design of heavy duty NC mathine tools are described. Amodule definition modelfor modular design and manufacturing of heavy duty NC machine tools isbulit and the essential composition of the module definition model (MDM) is discussed in detail. Itis composed of two models: the part definition model (PDM) and the module assembly model(MAM). The PDM and MAM are built and their structures are given. Using object-oriented know-ledge representation and based on these models, an intelligent support system of modular design forheavy duty NC machine tools is developed and implemented This system has been applied to thepractical use of Wuhan Heavy Duty Machine Tool Works
文摘The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and productive traits such as growth rate and carcass composition. As yet system- atical research on SLA expression profile is not reported. In order to illustrate SLA expression comprehensively and deepen our understanding of its function, we outlined the expression profile of SLA in 51 tissues of Landrace by ana- lyzing a large amount of ESTs produced by “Sino-Danish Porcine Genome Project”. In addition, we also compared the expression profile of SLA in several tissues from different development stages and from another breed (Erhualian). The result shows: (i) classical SLA genes are highly expressed in immune tissues and middle part of intestine; (ii) although SLA-3 is an SLA Ia gene, its expression abundance and pat- tern are quite different from those of the other two SLA Ia genes. The same phenomenon is seen in HLA-C expression, suggesting that the two genes may function similarly and undergo convergent evolution; (iii) except in jejunum, the antigen presenting genes are more highly expressed in breed Erhualian than in Landrace. The difference might associate with the higher resistance to bad conditions (including pathogens) of Erhualian and higher growth rates of Land- race.
文摘A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.
