Genetic diversity in eggplant(Solanum melongena L.)germplasm from three secondary geographical origins of diversity using SSR markers

Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity.In this study,the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization.The estimation of genetic diversity among the population of three countries(Bangladesh,Malaysia,and Thailand)varied from 0.57 to 0.74,with Shannon’s index value of 0.65.The mean value of expected heterozygosity and Nei’s index was 0.49,with an average PIC value of 0.83.A dendrogram was constructed based on UPGMA(unweighted pair group method with arithmetic mean),and the dendrogram categorized all accessions into six groups.The AMOVA(analysis of molecular variance)revealed a 77%total variation within the population from three different countries and 23%total variation among the populations.The result revealed a high genetic differentiation among the eggplant germplasms while the accessions that are farther from each other show a high level of diversity;thus,they can be recommended as parental in breeding programs.Hence,accessions,EB12,ET11,ET13,ET15,ET16,and ET17 could be crossed with accessions EM3,EB34,and EB3 for improvement in the future breeding program.

Development of anthracnose disease resistance and heat tolerancechili through conventional breeding and molecular approaches:a review

Chili(Capsicum annuum L.)is the popular spicy vegetable crops belonging to family Solanaceae.Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper.Chili is used as spices,folk remedies for diseases,vegetables,and coloring agent showing a diverse role in human’s life.However,its production is hampered by different biotic stress and abiotic factors.Similarly,the unavailability of high yielding varieties,high temperature,and disease incidence,particularly,anthracnose disease,are the major constraints responsible for the low production of chili pepper.The advents of molecular markers,advancement in quantitative trait loci by classical genetic analysis,and conventional breeding have shown the number of genes for many important and major traits.While the newly developed genotyping technologies and nextgeneration sequencing have led to the discovery of molecular basis for economic important characters in the chili genome and generate large scale data for genomic resources.Based on this background,this review summarizes progress in the development of anthracnose disease-resistant and heat-tolerant chili genotypes through conventional breeding and molecular approaches.This review would help plant breeders in understanding the phenotypic and genetic make-up of capsicum genotypes and provides opportunities for pyramiding two respected genes with the help of diversified phenotypic and molecular marker evaluation.

Development of high yield and tomato yellow leaf curl virus(TYLCV)resistance using conventional and molecular approaches:A review

Tomato(Solanum lycopersicum L.)belonging to the family Solanaceae is the second most consumed and cultivated vegetable globally.Since the ancient time of its domestication,thousands of cultivated tomato varieties have been developed targeting an array of aspects.Among which breeding for yield and yield-related traits are mostly focused.Cultivated tomato is extremely genetically poor and hence it is a victim for several biotic and abiotic stresses.Among the biotic stresses,the impact of viral diseases is critical all over tomato cultivating areas.Improvement of tomato still largely rely on conventional methods worldwide while molecular approaches,particularly Marker Assisted Selection(MAS)has become popular across the globe as a fast,low cost and precise tool which is essential in present day plant breeding.In this review paper,breeding tomato for high yield and viral disease resistance,particularly to tomato yellow leaf curl virus disease(TYLCVD)using conventional and molecular approaches will be discussed.Lining up of this set of information will be useful to those who are interested in tomato variety development with high yielding and TYLCVD resistance.