同伴支持教育对慢性萎缩性胃炎患者健康行为及生活质量的影响

背景慢性萎缩性胃炎为临床常见疾病,其病因及发病机制较为复杂,不仅与某些病理因素有关,个体对疾病的认知水平、心理状态、健康行为方式等也与之有关.从心理学角度来讲,适当的心理管理在疾病管理中的意义显著,尤其是在慢性疾病管理中的效果值得肯定.本研究以同伴支持教育为主要干预手段,分析其对慢性萎缩性胃炎患者健康行为及生活质量的影响.目的探讨同伴支持教育对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)患者健康行为及生活质量的影响.方法选择2017-01/2017-12符合标准的CAG患者107例为研究对象,采用随机数字表法分为干预组55例、对照组52例.对照组给予包括疾病认知、健康行为、并发症预防等在内的常规健康教育,干预组联合应用同伴支持教育.随访6 mo,比较两组患者健康行为、生活质量、健康教育满意度等指标.结果干预组CAG患者合理膳食、运动锻炼、情绪控制、戒烟戒酒达标率明显高于对照组(87.27%vs 71.15%,89.09%vs 73.08%,85.45%vs 69.23%,89.09%vs 71.15%,χ2=4.252,4.510,4.044,5.4582,P<0.05);生理职能、总体健康、活力、社会功能、情感职能、精神健康评分明显高于对照组(76.62±8.21 vs 70.10±8.32,67.45±7.26 vs 61.24±7.32,70.12±8.23 vs 62.45±7.41,82.45±8.36 vs 76.45±8.45,73.12±8.42 v s 65.12±7.56,72.65±8.22 v s 64.78±8.34,t=4.079,4.405,5.056,3.691,5.161,4.915,P<0.05),健康教育满意度96.36%明显高于对照组84.62%(χ2=4.354,P<0.05).结论同伴支持教育更容易被CAG患者所接受,有助于促进患者健康行为方式的养成,进而改善患者生活质量.

Disruption of Secondary Wall Cellulose Biosynthesis Alters Cadmium Translocation and Tolerance in Rice Plants

Tricheary elements （TEs）, wrapped by secondary cell wall, play essential roles in water, mineral, and nutrient transduction. Cadmium （Cd） is a toxic heavy metal that is absorbed by roots and transported to shoot, leaves, and grains through vascular systems in plants. As rice is a major source of Cd intake, many efforts have been made to establish ＇low- Cd rice＇. However, no links have been found between cellulose biosynthesis and cadmium accumulation. We report here a rice brittle culm13 mutant, resulting from a novel missense mutation （G101K） in the N-terminus of cellulose synthase subunit 9 （CESA9）. Except for the abnormal mechanical strength, the mutant plants are morphologically indistinguishable from the wild-type plants. Transmission electron microscopy （TEM） and chemical analyses showed a slight reduction in secondary wall thickness and 22% decrease in cellulose content in bc13 plants. Moreover, this mutation unexpectedly confers the mutant plants Cd tolerance due to less Cd accumulation in leaves. Expression analysis of the genes required for Cd uptake and transport revealed complicated alterations after applying Cd to wild-type and bc13. The mutants were further found to have altered vascular structure. More importantly, Cd concentration in the xylem saps from the bc13 plants was significantly lower than that from the wild-type. Combining the analyses of CESA9 gene expression and Cd content retention in the cell-wall residues, we conclude that CESA9^G101K mutation alters cell-wall properties in the conducting tissues, which consequently affects Cd translocation efficiency that largely contributes to the low Cd accumulation in the mutant plants.

Expression of Cytokeratin,Actin and Endocrine Hormones in Human Hatched Blastocyst Implantation Model in vitro

Objective To investigate the expression of cytokeratin,actin and hCG,E2 and P of human hacthed blastocysts in the model.Methods Human hatched blastocysts were co-cultured with human endometrial decidualization stromal cell monolayer.The process of orientation,attachment,outgrowth and invasion in morphology were observed.Immunofluorescence staining for cytokeratin and actin,immunofluorescence measurement of hCG and radioimmunoassay measurement of E2 and P were performed.Results Blastocysts attached to stromal cell layer after 5h in co-culture.After 24h in co-culture,the trophoblast protruded from two opposite poles of the blastocyst and underwent outgrowth into the stromal cell monolayer,blastocyst became bigger and invaded finally into the stromal cells.After 48h in co-culture,cytokeratin staining was only visible in trophoblast but not stromal cells,actin staining was visible in both of trophoblast and stromal cells with distinct conformation and structure.Stromal cells had prominent linear actin filaments,aligned along the long axis of the cells.Cytokeratin staining in trophoblast cells was localized in short filaments arranged in a mesh.hCG,E2 and P levels in the supernate of stromal cell-blastocyst co-culture were higher than the supernate from blastocyst cultured only （P〈0.01）.Conclusion An implantation model for the reflection of the process of human blastocysts attachment,outgrowth and invasion into stromal cells has been established in vitro.Cytokeratin,actin and hCG,E2 and P take place corresponding changes in the human implantation blastocyst cells.