Objective To investigate the social mental state of drug addicts in a compulsive drug abuse treatment center; evaluate the effectiveness of integrated program for the prevention of abuse relapse and improvement of dru...Objective To investigate the social mental state of drug addicts in a compulsive drug abuse treatment center; evaluate the effectiveness of integrated program for the prevention of abuse relapse and improvement of drug addicts＇ psychological health. Methods The study subjects were addicts from the Wuhan Compulsive Drug Abuse Treatment Center between October 2003 and June 2004, who satisfied the inclusion criteria. A non-randomized control-intervention study design was adopted. Volunteers willing to take part in intervention were put into the intervention group with their full awareness and willingness to prevent drug abuse relapse. The control group was composed of the addicts who were willing to prevent relapse and to be followed up after their discharge. Results The effectiveness of the integrated intervention program in promoting addicts＇ psychological health： before the intervention, the scores of Self-Rating Anxiety Scale （SAS）, the positive and negative dimensionalities of Simple Coping Style Questionnaire （SCSQ） and Chinese Perceived Stress Scales （CPSS） had no significant differences between the intervention group and the control group. After the intervention, except that the SCSQ＇s positive dimensionality in the intervention group was significantly higher than that in the control group, other indices in the intervention group were lower. Before and after the intervention, the psychological health level in both the groups was lower than that in the normal population; there were significant differences between addicts and normal subjects in regards with all of the indices above. Conclusion Drug abuse was associated closely with addicts＇ social mental factors. The integrated intervention program can alleviate anxiety and stress, reduce co-morbid mental disorders and effectively improve their coping style. In conclusion, the program can promote addicts＇ psychological health significantly.展开更多
AZ31 magnesium alloy sheets with different strong textures were cryorolled at the liquid-nitrogen temperature to the strain of 4% and 8%. The microstructure and texture of the rolled sheets were investigated via scann...AZ31 magnesium alloy sheets with different strong textures were cryorolled at the liquid-nitrogen temperature to the strain of 4% and 8%. The microstructure and texture of the rolled sheets were investigated via scanning electron microscopy（SEM）, electron backscatter diffraction（EBSD）, and X-ray diffraction（XRD）. The mechanical properties of the sheets were tested through in-plane uniaxial tensile tests at ambient temperature. The tensile stress was exerted in the rolling direction（RD） and transverse directions（TD）. The microstructural and textural evolutions of the alloy during cryorolling were investigated. Due to active twining during rolling, the initial texture significantly influenced the microstructural and textural evolutions of the rolled sheets. A {10 12} extension twin was found as the dominated twin-type in the cryorolled samples. After cryogenic rolling, the ductility of the samples decreased while the strength increased. Twinning also played an important role in explaining the mechanical differences between the rolled samples with different initial textures. The samples were significantly strengthened by the high stored energy accumulated from cryorolling.展开更多
Background Newborn screening(NBS)is an important and successful public health program that helps improve the long-term clinical outcomes of newborns by providing early diagnosis and treatment of certain inborn disease...Background Newborn screening(NBS)is an important and successful public health program that helps improve the long-term clinical outcomes of newborns by providing early diagnosis and treatment of certain inborn diseases.The develop-ment of next-generation sequencing(NGS)technology provides new opportunities to expand current newborn screening methodologies.Methods We designed a a newborn genetic screening(NBGS)panel targeting 135 genes associated with 75 inborn disorders by multiplex PCR combined with NGS.With this panel,a large-scale,multicenter,prospective multidisease analysis was conducted on dried blood spot(DBS)profiles from 21,442 neonates nationwide.Results We presented the positive detection rate and carrier frequency of diseases and related variants in different regions;and 168(0.78%)positive cases were detected.Glucose-6-Phosphate Dehydrogenase deficiency(G6PDD)and phenylketonuria(PKU)had higher prevalence rates,which were significantly different in different regions.The positive detection of G6PD variants was quite common in south China,whereas PAH variants were most commonly identified in north China.In addi-tion,NBGS identified 3 cases with DUOX2 variants and one with SLC25A13 variants,which were normal in conventional NBS,but were confirmed later as abnormal in repeated biochemical testing after recall.Eighty percent of high-frequency gene carriers and 60%of high-frequency variant carriers had obvious regional differences.On the premise that there was no significant difference in birth weight and gestational age,the biochemical indicators of SLC22A5 c.1400C>G and ACADSB c.1165A>G carriers were significantly different from those of non-carriers.Conclusions We demonstrated that NBGS is an effective strategy to identify neonates affected with treatable diseases as a supplement to current NBS methods.Our data also showed that the prevalence of diseases has significant regional charac-teristics,which provides a theoretical basis for screening diseases in different regions.展开更多
The poor stability of therapeutic peptides in physiological environments hampers their therapeutic efficacy.In this work,a strategy of supramolecular peptide therapeutics(SPT)was proposed for the improvement of the st...The poor stability of therapeutic peptides in physiological environments hampers their therapeutic efficacy.In this work,a strategy of supramolecular peptide therapeutics(SPT)was proposed for the improvement of the stability and anticancer efficacy of the peptides in vivo.N-Terminal phenylalanine-containing cytotoxic peptides were carried in the cucurbit[7]uril-containing polymer through host–guest interactions between the phenylalanine and cucurbit[7]uril,generating the supramolecular peptide complex with high peptide encapsulation efficiency(>97%).The formation of the supramolecular peptide complex reserved the biological activities of the peptide,presenting prolonged blood circulation and improved anticancer efficacy.This SPT strategy might provide a cucurbituril-and phenylalanine-functionalized approach for the design and the development of peptide-based pharmaceuticals.展开更多
Radionuclide imaging is now the premier imaging method in clinical practice for its high sensitivity and tomographic capability.Current clinically available radio imaging methods mostly use positron-emission tomograph...Radionuclide imaging is now the premier imaging method in clinical practice for its high sensitivity and tomographic capability.Current clinically available radio imaging methods mostly use positron-emission tomography(PET)and single-photon emission computed tomography(SPECT)to detect anatomic abnormalities that conventional imaging techniques typically have challenges for visualizing.Contrast agents are indispensable for radionuclide imaging,and the radionuclide is always attached to a suitable vector that achieves targeted delivery.Nowadays,peptides have attracted increasing interest in targeting vectors of contrast agents,mainly due to their high specificity for target receptors at nanomolar concentrations and low toxicity.Radiolabeled peptide probes as kinds of PET/SPECT tracers had become essential tools for clinical radionuclide diagnosis.This review mainly summarizes radiolabeled peptide probes for bioimaging,including fundamental concepts of radiolabeled peptide probe design,some typical peptide analogs radiocontrast agents for PET,SPECT,and the combination imaging.展开更多
基金a grant from the National Natural Science Foundation (No.30271140, 2002)
文摘Objective To investigate the social mental state of drug addicts in a compulsive drug abuse treatment center; evaluate the effectiveness of integrated program for the prevention of abuse relapse and improvement of drug addicts＇ psychological health. Methods The study subjects were addicts from the Wuhan Compulsive Drug Abuse Treatment Center between October 2003 and June 2004, who satisfied the inclusion criteria. A non-randomized control-intervention study design was adopted. Volunteers willing to take part in intervention were put into the intervention group with their full awareness and willingness to prevent drug abuse relapse. The control group was composed of the addicts who were willing to prevent relapse and to be followed up after their discharge. Results The effectiveness of the integrated intervention program in promoting addicts＇ psychological health： before the intervention, the scores of Self-Rating Anxiety Scale （SAS）, the positive and negative dimensionalities of Simple Coping Style Questionnaire （SCSQ） and Chinese Perceived Stress Scales （CPSS） had no significant differences between the intervention group and the control group. After the intervention, except that the SCSQ＇s positive dimensionality in the intervention group was significantly higher than that in the control group, other indices in the intervention group were lower. Before and after the intervention, the psychological health level in both the groups was lower than that in the normal population; there were significant differences between addicts and normal subjects in regards with all of the indices above. Conclusion Drug abuse was associated closely with addicts＇ social mental factors. The integrated intervention program can alleviate anxiety and stress, reduce co-morbid mental disorders and effectively improve their coping style. In conclusion, the program can promote addicts＇ psychological health significantly.
基金financially supported by the National Natural Science Foundation of China(No.51401019)the China Postdoctoral Science Foundation(No.2014M550612)+1 种基金the Fundamental Research Funds for the Central Universities(Nos.FRF-TP-14-048A1 and FRF-TP-15-055A2)the Common Construction Project from Beijing Municipal Commission of Education(No.FRF-SD-13-005B)
文摘AZ31 magnesium alloy sheets with different strong textures were cryorolled at the liquid-nitrogen temperature to the strain of 4% and 8%. The microstructure and texture of the rolled sheets were investigated via scanning electron microscopy（SEM）, electron backscatter diffraction（EBSD）, and X-ray diffraction（XRD）. The mechanical properties of the sheets were tested through in-plane uniaxial tensile tests at ambient temperature. The tensile stress was exerted in the rolling direction（RD） and transverse directions（TD）. The microstructural and textural evolutions of the alloy during cryorolling were investigated. Due to active twining during rolling, the initial texture significantly influenced the microstructural and textural evolutions of the rolled sheets. A {10 12} extension twin was found as the dominated twin-type in the cryorolled samples. After cryogenic rolling, the ductility of the samples decreased while the strength increased. Twinning also played an important role in explaining the mechanical differences between the rolled samples with different initial textures. The samples were significantly strengthened by the high stored energy accumulated from cryorolling.
基金the Foundation of National Key R&D Program of China of Research on Application Demonstration and Evaluation of Comprehensive Prevention And Control Technology of Birth Defects(Grant No.2018YFC1002700)Zhejiang R&D Research Project Research on New Technologies for Birth Health,Birth Safety and Perinatal Disease Diagnosis and Treatment(Grant No.2021C03099).
文摘Background Newborn screening(NBS)is an important and successful public health program that helps improve the long-term clinical outcomes of newborns by providing early diagnosis and treatment of certain inborn diseases.The develop-ment of next-generation sequencing(NGS)technology provides new opportunities to expand current newborn screening methodologies.Methods We designed a a newborn genetic screening(NBGS)panel targeting 135 genes associated with 75 inborn disorders by multiplex PCR combined with NGS.With this panel,a large-scale,multicenter,prospective multidisease analysis was conducted on dried blood spot(DBS)profiles from 21,442 neonates nationwide.Results We presented the positive detection rate and carrier frequency of diseases and related variants in different regions;and 168(0.78%)positive cases were detected.Glucose-6-Phosphate Dehydrogenase deficiency(G6PDD)and phenylketonuria(PKU)had higher prevalence rates,which were significantly different in different regions.The positive detection of G6PD variants was quite common in south China,whereas PAH variants were most commonly identified in north China.In addi-tion,NBGS identified 3 cases with DUOX2 variants and one with SLC25A13 variants,which were normal in conventional NBS,but were confirmed later as abnormal in repeated biochemical testing after recall.Eighty percent of high-frequency gene carriers and 60%of high-frequency variant carriers had obvious regional differences.On the premise that there was no significant difference in birth weight and gestational age,the biochemical indicators of SLC22A5 c.1400C>G and ACADSB c.1165A>G carriers were significantly different from those of non-carriers.Conclusions We demonstrated that NBGS is an effective strategy to identify neonates affected with treatable diseases as a supplement to current NBS methods.Our data also showed that the prevalence of diseases has significant regional charac-teristics,which provides a theoretical basis for screening diseases in different regions.
基金supported financially by the Ministry of Science and Technology of China(2018YFA0208900 and 2018YFE0205400)the National Natural Science Foundation of China(21821001,21805058,and 51890892).
文摘The poor stability of therapeutic peptides in physiological environments hampers their therapeutic efficacy.In this work,a strategy of supramolecular peptide therapeutics(SPT)was proposed for the improvement of the stability and anticancer efficacy of the peptides in vivo.N-Terminal phenylalanine-containing cytotoxic peptides were carried in the cucurbit[7]uril-containing polymer through host–guest interactions between the phenylalanine and cucurbit[7]uril,generating the supramolecular peptide complex with high peptide encapsulation efficiency(>97%).The formation of the supramolecular peptide complex reserved the biological activities of the peptide,presenting prolonged blood circulation and improved anticancer efficacy.This SPT strategy might provide a cucurbituril-and phenylalanine-functionalized approach for the design and the development of peptide-based pharmaceuticals.
基金supported by the National Key R&D Program of China(No.2018YFE0205400)。
文摘Radionuclide imaging is now the premier imaging method in clinical practice for its high sensitivity and tomographic capability.Current clinically available radio imaging methods mostly use positron-emission tomography(PET)and single-photon emission computed tomography(SPECT)to detect anatomic abnormalities that conventional imaging techniques typically have challenges for visualizing.Contrast agents are indispensable for radionuclide imaging,and the radionuclide is always attached to a suitable vector that achieves targeted delivery.Nowadays,peptides have attracted increasing interest in targeting vectors of contrast agents,mainly due to their high specificity for target receptors at nanomolar concentrations and low toxicity.Radiolabeled peptide probes as kinds of PET/SPECT tracers had become essential tools for clinical radionuclide diagnosis.This review mainly summarizes radiolabeled peptide probes for bioimaging,including fundamental concepts of radiolabeled peptide probe design,some typical peptide analogs radiocontrast agents for PET,SPECT,and the combination imaging.
