A water-soluble polysaccharide, HM（41）, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM（41）...A water-soluble polysaccharide, HM（41）, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM（41） was composed of rhamnose（Rha）, arabinose（Ara）, xylose（Xyl）, mannose（Man）,galactose（Gal）, glucose（Glc） with a molar ratio of 1.0：5.5：1.8：3.0：9.4：21. The average molecular weight of HM（41） was approximately 1.17 ＊ 10~4. Periodate oxidation, Smith degradation, methylation and GC, IR,NMR, XRD, GC–MS analysis were used for the structural analysis of HM（41）. Its main chain was composed mainly of β-（1→4）Gal, β-（1→4）Glc and β-（1→6）Glc. β-（1 →4）Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues;（1→ 4）Glc had no branch;（1→6）Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of（1→3,6）-Rha,（1→4）/（1→5）-Ara,（1 →4）/（1→5）-Xyl,（1→4,6）-Man and（1→2）-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM（41） had strong scavenging activities in vitro hydroxyl. Overall, HM and HM（41） may have potential applications in the antioxidants for medical and food industry.展开更多
基金supported by the First-Class Discipline Construction Project for First-Class University of Minzu University of China (No. YLDX01013),111 Project (No. B08044)The ecological conservation technology integration and demonstration in north of Dzungaria (No. 2014BAC15B04)
文摘A water-soluble polysaccharide, HM（41）, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM（41） was composed of rhamnose（Rha）, arabinose（Ara）, xylose（Xyl）, mannose（Man）,galactose（Gal）, glucose（Glc） with a molar ratio of 1.0：5.5：1.8：3.0：9.4：21. The average molecular weight of HM（41） was approximately 1.17 ＊ 10~4. Periodate oxidation, Smith degradation, methylation and GC, IR,NMR, XRD, GC–MS analysis were used for the structural analysis of HM（41）. Its main chain was composed mainly of β-（1→4）Gal, β-（1→4）Glc and β-（1→6）Glc. β-（1 →4）Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues;（1→ 4）Glc had no branch;（1→6）Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of（1→3,6）-Rha,（1→4）/（1→5）-Ara,（1 →4）/（1→5）-Xyl,（1→4,6）-Man and（1→2）-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM（41） had strong scavenging activities in vitro hydroxyl. Overall, HM and HM（41） may have potential applications in the antioxidants for medical and food industry.
