With the development of color Doppler and laparoscopic ultrasound, now intraoperative ultrasound(IOUS) plays an important role in liver surgery. Compared to percutaneous ultrasound(US), IOUS is conducted directly on t...With the development of color Doppler and laparoscopic ultrasound, now intraoperative ultrasound(IOUS) plays an important role in liver surgery. Compared to percutaneous ultrasound(US), IOUS is conducted directly on the liver surface, with no blind spots or dead ends, thus improves the detection, localization and characterization of lesions without influencing factors, such as obesity.展开更多
The aim of this study was to investigate the role of seminal plasma miR-210-3p in the impairment of semen quality caused by varicocele.This study included 102 patients whose semen quality was normal when they were dia...The aim of this study was to investigate the role of seminal plasma miR-210-3p in the impairment of semen quality caused by varicocele.This study included 102 patients whose semen quality was normal when they were diagnosed with varicocele.A 2-year follow-up for included patients was performed,and they were divided into Group A(semen quality became abnormal)and Group B(semen quality remained normal)according to the results of semen analysis during the follow-up.Semen parameters and seminal plasma miR-210-3p expression were investigated by semen analysis and quantitative real-time polymerase chain reaction,respectively.In vitro experiments with GC-2 cells were performed to explore the role of miR-210-3p in spermatogenic cells.The results of quantitative real-time polymerase chain reaction showed that the level of seminal plasma miR-210-3p in Group A was higher than that in Group B both after 2-year follow-up and when they were diagnosed with varicocele(both P<0.01).Apoptosis and proliferation assays showed that miR-210-3p induces apoptosis of spermatogenic cells by promoting caspase-3 activation.In conclusion,our study indicated that seminal plasma miR-210-3p induces spermatogenic cell apoptosis by activating caspase-3 in patients with varicocele.Seminal plasma miR-210-3p may be a potential biomarker for predicting impaired semen quality caused by varicocele.展开更多
文摘With the development of color Doppler and laparoscopic ultrasound, now intraoperative ultrasound(IOUS) plays an important role in liver surgery. Compared to percutaneous ultrasound(US), IOUS is conducted directly on the liver surface, with no blind spots or dead ends, thus improves the detection, localization and characterization of lesions without influencing factors, such as obesity.
基金by Tianjin Natural Science Foundation of China(No.17JCQNJC11900).
文摘The aim of this study was to investigate the role of seminal plasma miR-210-3p in the impairment of semen quality caused by varicocele.This study included 102 patients whose semen quality was normal when they were diagnosed with varicocele.A 2-year follow-up for included patients was performed,and they were divided into Group A(semen quality became abnormal)and Group B(semen quality remained normal)according to the results of semen analysis during the follow-up.Semen parameters and seminal plasma miR-210-3p expression were investigated by semen analysis and quantitative real-time polymerase chain reaction,respectively.In vitro experiments with GC-2 cells were performed to explore the role of miR-210-3p in spermatogenic cells.The results of quantitative real-time polymerase chain reaction showed that the level of seminal plasma miR-210-3p in Group A was higher than that in Group B both after 2-year follow-up and when they were diagnosed with varicocele(both P<0.01).Apoptosis and proliferation assays showed that miR-210-3p induces apoptosis of spermatogenic cells by promoting caspase-3 activation.In conclusion,our study indicated that seminal plasma miR-210-3p induces spermatogenic cell apoptosis by activating caspase-3 in patients with varicocele.Seminal plasma miR-210-3p may be a potential biomarker for predicting impaired semen quality caused by varicocele.
