Objective:To determine whether alpha lipoic acid（LA）can effectively protect lenses from hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>）-induced cataract.Methods:Lens from adult Sprague-Dawley...Objective:To determine whether alpha lipoic acid（LA）can effectively protect lenses from hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>）-induced cataract.Methods:Lens from adult Sprague-Dawley rats were cultured in 24-well plates and treated without or with 0.2 mM of H<sub>2</sub>O<sub>2</sub>,0.2 mM of H<sub>2</sub>O<sub>2</sub> plus 0.5 mM.1.0 mM.or 2.0 mM of LA for 24 h.Cataract was assessed using cross line grey scale measurement.Superoxide dismutase（SOD）.glutathione（GSH-Px）.lactate dehydrogenase（LDH）. and maloudialdehyde（MDA）activity or level in lens homogenates was measured.Apoptosis of lens epithelial cells in each group were detected by Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling（TUNEL） Assay.Results:A total of 0.2 mM of H<sub>2</sub>O<sub>2</sub> induced obvious cataract formation and apoptosis in lens’ epithelial cells,but 0.5-2.0 mM of LA could block the effect of 0.2 mM H<sub>2</sub>O<sub>2</sub> in inducing cataract and apoptosis.Furthermore.0.2 mM ol H<sub>2</sub>O<sub>2</sub> significantly decreased SOD.GSH-Px,and LDH activity and significant increased MDA level in the lens,but 0.5-2.0 mM of LA blocked the effect of 0.2 mM H<sub>2</sub>O<sub>2</sub>.One mM of LA was found to be the most effective. Conclusions:LA can protect lens from H<sub>2</sub>O<sub>2</sub>-induced cataract.LA exerts protective effects through inhibition of lens’ epithelial cell apoptosis and activation of anti-oxidative enzymes.展开更多
文摘Objective:To determine whether alpha lipoic acid（LA）can effectively protect lenses from hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>）-induced cataract.Methods:Lens from adult Sprague-Dawley rats were cultured in 24-well plates and treated without or with 0.2 mM of H<sub>2</sub>O<sub>2</sub>,0.2 mM of H<sub>2</sub>O<sub>2</sub> plus 0.5 mM.1.0 mM.or 2.0 mM of LA for 24 h.Cataract was assessed using cross line grey scale measurement.Superoxide dismutase（SOD）.glutathione（GSH-Px）.lactate dehydrogenase（LDH）. and maloudialdehyde（MDA）activity or level in lens homogenates was measured.Apoptosis of lens epithelial cells in each group were detected by Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling（TUNEL） Assay.Results:A total of 0.2 mM of H<sub>2</sub>O<sub>2</sub> induced obvious cataract formation and apoptosis in lens’ epithelial cells,but 0.5-2.0 mM of LA could block the effect of 0.2 mM H<sub>2</sub>O<sub>2</sub> in inducing cataract and apoptosis.Furthermore.0.2 mM ol H<sub>2</sub>O<sub>2</sub> significantly decreased SOD.GSH-Px,and LDH activity and significant increased MDA level in the lens,but 0.5-2.0 mM of LA blocked the effect of 0.2 mM H<sub>2</sub>O<sub>2</sub>.One mM of LA was found to be the most effective. Conclusions:LA can protect lens from H<sub>2</sub>O<sub>2</sub>-induced cataract.LA exerts protective effects through inhibition of lens’ epithelial cell apoptosis and activation of anti-oxidative enzymes.
