A moderate stress such as cold water swimming can raise the tolerance of the body to potentially injurious events. However, little is known about the mechanism of beneficial effects induced by moderate stress. In this...A moderate stress such as cold water swimming can raise the tolerance of the body to potentially injurious events. However, little is known about the mechanism of beneficial effects induced by moderate stress. In this study, we used a classic rat model of traumatic brain injury to test the hypothesis that cold water swimming preconditioning improved the recovery of cognitive functions and explored the mechanisms. Results showed that after traumatic brain injury, pre-conditioned rats（cold water swimming for 3 minutes at 4℃） spent a significantly higher percent of times in the goal quadrant of cold water swim, and escape latencies were shorter than for non-pretreated rats. The number of circulating endothelial progenitor cells was significantly higher in pre-conditioned rats than those without pretreatment at 0, 3, 6 and 24 hours after traumatic brain injury. Immunohistochemical staining and Von Willebrand factor staining demonstrated that the number of CD34~＋ stem cells and new blood vessels in the injured hippocampus tissue increased significantly in pre-conditioned rats. These data suggest that pretreatment with cold water swimming could promote the proliferation of endothelial progenitor cells and angiogenesis in the peripheral blood and hippocampus. It also ameliorated cognitive deficits caused by experimental traumatic brain injury.展开更多
The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea E...The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.展开更多
基金supported by a grant from the Incubation Project of Natural Science Foundation of Tianjin Medical University General Hospital in China,No.303071901401the Natural Science Foundation of Tianjin of China,No.13JCZDJC30800the National Natural Science Foundation of China,No.81271361 and 81330029
文摘A moderate stress such as cold water swimming can raise the tolerance of the body to potentially injurious events. However, little is known about the mechanism of beneficial effects induced by moderate stress. In this study, we used a classic rat model of traumatic brain injury to test the hypothesis that cold water swimming preconditioning improved the recovery of cognitive functions and explored the mechanisms. Results showed that after traumatic brain injury, pre-conditioned rats（cold water swimming for 3 minutes at 4℃） spent a significantly higher percent of times in the goal quadrant of cold water swim, and escape latencies were shorter than for non-pretreated rats. The number of circulating endothelial progenitor cells was significantly higher in pre-conditioned rats than those without pretreatment at 0, 3, 6 and 24 hours after traumatic brain injury. Immunohistochemical staining and Von Willebrand factor staining demonstrated that the number of CD34~＋ stem cells and new blood vessels in the injured hippocampus tissue increased significantly in pre-conditioned rats. These data suggest that pretreatment with cold water swimming could promote the proliferation of endothelial progenitor cells and angiogenesis in the peripheral blood and hippocampus. It also ameliorated cognitive deficits caused by experimental traumatic brain injury.
基金supported by the Zhejiang Provincial Science and Technol-ogy Program (No. 2007C32013)the Zhejiang Provincial Natural Science Foundation (No. Y3090660) of China
文摘The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.
