BRMS1 performs an anti-tumor effect on renal cell carcinoma via inhibition of PI3K/AKT/mTOR signaling pathway

Objective:To explore the anti-tumor of breast cancer metastasis suppressor 1(BRMS1)on renal cell carcinoma.Methods:BRSM1 stabilized overexpressed and knockdown cell models were constructed to detect the effect of BRMS1 overexpression and knockdown on viability rate of 769-P cells.Activation of PI3K/AKT-mTOR-NFκB signaling pathway,expression of cellular apoptosis-related proteins,DNA damage repair-related proteins and angiogenesis-related proteins were detected using Western blotting analysis.Results:The viability rate of 769-P cells were significantly decreased in BRMS1 overexpression cells while significantly increased in BRMS1 knockdown cells(P<0.05).The activity of PI3K/AKT-mTOR-NFκB signaling pathway was significantly inhibited with BRMS1 overexpression(P<0.05),and expression of BAX was significantly increased with significantly decreased expression of Bcl-2(P<0.05).Besides,BRMS1 overexpression could significantly decrease the expression of DNA damage repair-related proteins and angiogenesis and cell-matrix degradation related proteins(P<0.05).Conclusion:BRMS1 induces apoptosis of renal cell carcinoma and performs an anti-tumor effect via inhibition of PI3K/AKT-mTOR-NFκB signaling pathway,expression of DNA damage repair-related proteins and angiogenesis-related proteins.

Spinal Cord Kinking in Thoracic Myelopathy Caused by Ossification of the Ligamentum Flavum

Background：Ossification of the ligamentum flavum （OLF） is being increasingly recognized as a cause of thoracic myelopathy.This study was to describe a rare clinical entity of spinal cord kinking （SK） in thoracic myelopathy secondary to OLF.Methods：The data of 95 patients with thoracic myelopathy secondary to OLF were analyzed retrospectively.The incidence and location of SK were determined using preoperative magnetic resonance imaging （MRI）.The clinical presentation and radiological characteristics in patients with SK were analyzed.Posterior en bloc laminectomy with OLF was performed,and the surgical results were evaluated.Results：SK was found in seven patients （7.4％） based on preoperative MRI.The patients included one male and six females with an average age of 55.6 years （range,48-64 years）.Five patients presented with radiculomyelopathy and two presented with typical thoracic myelopathy of spastic paraparesis.In all cases,the kinking was located just above the end of the spinal cord where the conus medullaris （CM） was compressed by the OLF.The degree of SK varied from mild to severe.The tip of the CM was located between the upper third of T1 1 to the lower third of L 1,above the lower edge of L 1.With an average follow-up of 30.4 months,the modified Japanese Orthopedic Association score significantly improved from 5.7 ± 1.8 preoperatively to 8.9 ± 1.4 postoperatively （t =12.05;P ＜ 0.0001） with an improvement rate of 63.1 ± 12.3％.Conclusions：SK is a rare radiological phenomenon.It is typically located at the thoracolumbar junction,where the CM is compressed by the OLF.Our findings indicate that these patients may benefit from a posterior decompressive procedure.