不同产地牛蒡子的指纹图谱研究

应用HPLC对九个不同产地牛蒡子药材进行指纹图谱研究。使用岛津ODS柱(4.6mm×215;150mm,5μm),以乙腈–水为流动相,梯度洗脱,流动相流速:1.0mL-min-1,柱温:25℃,检测波长:280nm,得到11个共有峰,建立了牛蒡子药材的HPLC指纹图谱可用于牛蒡子药材的专属性鉴别,九个不同产地牛蒡子药材HPLC指纹图谱中,其相似度都大于0.99,说明不同产地的牛蒡子其质量的均一性很好。

A brief review of forensically important flesh flies (Diptera: Sarcophagidae)

Forensic entomology could provide valuable data for the minimum postmortem interval (PMImin) estimation and other relevant information,such as causes and circumstances of death.Some representatives of flesh flies are one of the dominant necrophagous insects during early stages of decomposition,demonstrating unique biological characteristics compared with other necrophagous flies.Moreover,they lead to global health concerns as carriers of various pathogenic micro-organisms,and dominantly result in the traumatic myiasis.Thus,sarcophagid flies are considered important in decomposition processes for PMImin estimation.However,the utility of sarcophagid flies has been seriously hampered by limited ecological,biological and taxonomic knowledge of them.The aim of this paper is to provide a brief review on the species,distribution and biological habit of forensically important sarcophagid flies.In addition,the relation between traumatic myiasis and flesh flies,molecular identification methods and developmental pattern of flesh flies are summarized.

Regulatory variation within 3’UTR of STAT5A correlates with sudden cardiac death in Chinese populations

Definitive diagnosis to sudden cardiac death(SCD)is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death.It is therefore important to uncover the inherited risk component to SCD.Signal transducer and activators of transcription 5 A(STAT5A)is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular processes.In this study,we performed a systematic variant screening on the STAT5A to filter potential functional genetic variations.Based on the screening results,an insertion/deletion polymorphism(rs3833144)in 3’UTR of STAT5A was selected as the candidate variant.A total of 159 SCD cases and 668 SCD matched healthy controls was enrolled to perform a case-control study and evaluate the association between rs3833144 and SCD susceptibility in Chinese populations.Logistic regression analysis showed that the deletion allele of rs3833144 had significantly increased the SCD risk(odds ratio(OR)=1.54;95%confidence interval(CI)=1.18-2.01;P=0.000955).Further genotype-expression eQTL analysis showed that samples with deletion allele appeared to lower expression of STAT5A,and in silico prediction suggested the local 3 D structure changes of STAT5A mRNA caused by the variant.On the other hand,the bioinformatic analysis presented that promoters of RARA and PTGES3L-AARSD1 could interact with rs3833144,and eQTL analysis showed the higher expression of both genes in samples with deletion allele.Dual-luciferase activity assays also suggested the significant regulatory role of rs3833144 in gene transcription.Our current data thus suggested a possible involvement of rs3833144 to SCD predisposition in Chinese populations and rs3833144 with potential function roles may become a candidate marker for SCD diagnosis and prevention.

High-throughput sequencing to evaluate the effects of methamphetamine on the succession of the bacterial community to estimate the postmortem interval

In forensic medical examinations,estimating the postmortem interval(PMI)is an important factor.Methamphetamine(MA)is a synthetic stimulant that is commonly abused,and estimation of the PMI after MA abuse has become one of the main tasks in forensic investigation.Microorganisms play a vital role in carrion decomposition.Analysing the bacterial succession patterns can be used as a forensic tool to estimate the PMI.The present study aimed to analyse bacterial succession changes during the decomposition of MA to estimate the PMI.We analysed bacterial communities in rabbits treated with three different concentrations of MA(0,22.5,and 90 mg/kg)under the natural conditions of 20°C and 70%humidity by sequencing 16S rRNA gene amplicons using the Illumina MiSeq system.We obtained 2374209 high-quality sequences and 2937 operational taxonomic units(OTUs).The relative abundances of the bacterial communities varied markedly in response to different MA concentrations.Interestingly,in response to the different concentrations of MA,Bacteroidetes became disparate in the rectum in the late PMI.Increased numbers of bacterial taxa were identified in the rectum and buccal cavity samples,except at the highest concentration of MA in the rectum samples when PMI was 0-h,than were present in live rabbits.Meanwhile,the PMI correlated significantly with bacterial succession at different taxonomic levels.Our results suggested that bacterial community succession could be used as a“microbial clock”to estimate the PMI in cases of MA-related death;however,further study is required to gain a deeper understanding of this concept.

Fat Embolism as a Rare Complication of Large‑volume Liposuction in a Plastic Patient

Fat embolism is a dreaded complication in of procedures of multiple disciplines including plastic surgery.As the popularity of liposuction increases,cases of pulmonary embolism after liposuction are increasingly reported.However,documents of cerebral embolism after liposuction are rarely presented.The degree of disability with respect to Fat fat Embolism embolism Symdrome syndrome(FES)is a Gordian Knot in the evaluation of legal medicine.Therefore,it is of great significance for us to that we report the case of a 30‑year‑old woman who underwent a large‑volume liposuction that resulted in serious complications,especially cerebral embolism.Untypical symptoms of fat embolism hamper the clinical diagnosis,particularly after a surgical procedure such as liposuction.Such a seldom‑seen case would easily trigger medical disputes,especially in countries like China suffering increasing claims of medical malpractice and,medical negligences and lawsuits.Detailed descriptions of this case are presented below along with the discussion of the clinical symptoms and the diagnostic approaches to fat embolism.