Effects of Perioperative Pain Intervention on Postoperative Rehabilitation of Patients with Partial Resection of Lung Cancer

Objective: To explore the effect of perioperative pain intervention on postoperative rehabilitation of patients who underwent thoracoscopic partial resection of lung cancer. Methods: From January 2021 to May 2021, 100 patients with primary lung cancer who underwent thoracoscopic partial lung resection in Cardiopulmonary Department II of Cancer Center in our hospital were selected. They were divided into observation group and control group by random number table. Routine nursing after surgery was used in both groups, the observation group was given perioperative pain intervention nursing on the basis of routine nursing, and the postoperative pain (6 h, 12 h, 24 h, 48 h after operation), the rate of out-of-bed activity within 24 h after operation, lien chest tube time, the incidence of postoperative complications, the influence of pain on daily life, the satisfaction of patients with pain control methods and pain education and the satisfaction of discharged patients were observed and recorded. Results: There was no significant difference in general data (age, sex, educational level, course of disease, TNM stage of lung cancer, maximum diameter of tumor (CM), surgical site) between the two groups (P > 0.05);the NRS scores of the observation group at 6, 12, 24 and 48 hours after operation were all lower than those in the control group, and the difference was statistically significant (P < 0.05);after operation, the rate of 24 h out-of-bed activity in the observation group was higher than that in the control group, the lien chest tube time was shorter than the control group, and the incidence of postoperative complications was lower than that in the control group, the difference was statistically significant (P < 0.05);after operation, the effect level of pain in the observation group was lower than that in the control group, and the satisfaction of pain health education, pain control methods and discharged patients was higher than that in the control group, with statistical significance (P < 0.05). Conclusion: Perioperative pain intervention can effectively relieve postoperative pain state of patients, promote patients’ early out of bed and conducive to lung expansion, shorten the time of lien chest tube, reduce postoperative complications and the impact of pain on daily life, help patients recover as soon as possible, and improve the satisfaction of patients for medical treatment.

Activation of TLR7 increases CCND3 expression via the downregulation of miR-15b in B cells of systemic lupus erythematosus

Systemic lupus erythematosus （SLE） is an autoimmune disease characterized by B-cell hyperreactivity. The Toll-like receptor 7 （TLR7） signaling pathway is abnormally activated in SLE B cells. CyclinD3 （CCND3） plays an important role in B-cell proliferation, development, and differentiation. Although previous studies focused on the B cell-intrinsic role of TLR7 for the development of spontaneous germinal centers, the influence of TLR7 on CCND3 in SLE B cells is still not clear. Here, we used a B-cell profiling chip and found that CCND3 was related to SLE and significantly elevated in SLE B cells. Moreover, we determined that the expression level of CCND3 was higher, while miR-15b was significantly lower in the B cells from SLE patients and B6.MRL-Faslpr/J lupus mice compared to normal subjects. Furthermore, we demonstrated that the activation of TLR7 dramatically increased CCND3 expression but significantly decreased miR-15b in B cells in vitro and we identified that CCND3 is a direct target of miR-15b. To further confirm our results, we established another lupus model by topically treating C57BL/6 （B6） mice with the TLR-7 agonist imiquimod （IMQ） for 8 weeks according to the previously described protocol. Expectedly, topical treatment with I MQ also significantly increased CCND3 and decreased miR-15b in B cells of B6 mice. Taken together, our results identified that the activation of TLR7 increased CCND3 expression via the downregulation of miR-15b in B cells; thus, these findings suggest that extrinsic factor-induced CCND3 expression may contribute to the abnormality of B cell in SLE.