Diabetes mellitus is a metabolic disorder syndrome caused by various pathogenic factors leading to imbalance of active substance secretion and endothelial dysfunction,causing macro and micro vascular complications.As ...Diabetes mellitus is a metabolic disorder syndrome caused by various pathogenic factors leading to imbalance of active substance secretion and endothelial dysfunction,causing macro and micro vascular complications.As the most important signal transduction molecule released by endothelium,NO participates in various physiological processes of microcirculation and plays an important role in vascular relaxation regulation.Extensive studies focus on the investigation of the analysis of NO transport in microvessels,RBC motion and its influence on NO transport since they are of extremely importance for understanding the mechanisms of vessel autoregulation and microcirculation lesion.NO transport characteristics are closely associated with hemorheology,hemodynamics and microvascular structure.Nevertheless,up to date,there are limited reports on NO transport characteristics in diabetic mellitus.The purpose of this review is to summarize the current advances in the investigation of NO transport characteristics from cellular to vascular level,which can provide effective ideas for the early diagnosis of vascular complications in diabetes.展开更多
[Objectives]To analyze the main chemical components in Cocculus laurifolius DC.by ultra-high performance liquid chromatography-quaternary rod/electrostatic field orbital hydrazine high resolution mass spectrometry.[Me...[Objectives]To analyze the main chemical components in Cocculus laurifolius DC.by ultra-high performance liquid chromatography-quaternary rod/electrostatic field orbital hydrazine high resolution mass spectrometry.[Methods]Using Welch AQ-C 18 chromatographic column(150 mm×2.1 mm,1.8μm),gradient elution was performed with 0.1%formic acid aqueous solution(A)-methanol(B)as the mobile phase,and electrospray ESI ionization source and simultaneous mass spectrometry scanning mode of positive and negative ions were used.[Results]26 kinds of chemical component were identified or inferred,including 3 organic acids,5 flavonoids,4 alkaloids,1 coumarin and 13 others.[Conclusions]The UPLC-Q-Exactive HRMS technique is simple,which lays a foundation for the drug-efficacy material basis and medicinal quality evaluation of C.laurifolius DC.展开更多
基金supported by National Natural Science Foundation of China(NSFC No 51576033,51976026)Dalian Innovative Funding of Science and Technology(No.2018J12SN076)the Fundamental Research Funds for the Central Universities(DUT20GJ203,DUT22YG206).
文摘Diabetes mellitus is a metabolic disorder syndrome caused by various pathogenic factors leading to imbalance of active substance secretion and endothelial dysfunction,causing macro and micro vascular complications.As the most important signal transduction molecule released by endothelium,NO participates in various physiological processes of microcirculation and plays an important role in vascular relaxation regulation.Extensive studies focus on the investigation of the analysis of NO transport in microvessels,RBC motion and its influence on NO transport since they are of extremely importance for understanding the mechanisms of vessel autoregulation and microcirculation lesion.NO transport characteristics are closely associated with hemorheology,hemodynamics and microvascular structure.Nevertheless,up to date,there are limited reports on NO transport characteristics in diabetic mellitus.The purpose of this review is to summarize the current advances in the investigation of NO transport characteristics from cellular to vascular level,which can provide effective ideas for the early diagnosis of vascular complications in diabetes.
基金Supported by Scientific Research Project of China Medical Association of Minorities(2022M2038-310401)Guangxi First-class Discipline Project for Traditional Chinese Medicine(GuiJiaoKeYan 202201)+3 种基金Scientific Research and Training Project for College Students of Guangxi University of Chinese Medicine(2023DXS14)Funding Project for High-level Innovation Team and Outstanding Scholars in Guangxi Universities(GuiJiaoRen 201407)NATCM s Project of High-level Construction of Key TCM Disciplines/Medicine for Ethnic Minorities(Zhuang Medicine)(ZYYZDXK-2023164)Guangxi Higher Education Key Laboratory for the Research of Toxic Diseases in Zhuang Medicine(GuiJiaoKeYan 202210).
文摘[Objectives]To analyze the main chemical components in Cocculus laurifolius DC.by ultra-high performance liquid chromatography-quaternary rod/electrostatic field orbital hydrazine high resolution mass spectrometry.[Methods]Using Welch AQ-C 18 chromatographic column(150 mm×2.1 mm,1.8μm),gradient elution was performed with 0.1%formic acid aqueous solution(A)-methanol(B)as the mobile phase,and electrospray ESI ionization source and simultaneous mass spectrometry scanning mode of positive and negative ions were used.[Results]26 kinds of chemical component were identified or inferred,including 3 organic acids,5 flavonoids,4 alkaloids,1 coumarin and 13 others.[Conclusions]The UPLC-Q-Exactive HRMS technique is simple,which lays a foundation for the drug-efficacy material basis and medicinal quality evaluation of C.laurifolius DC.
