Current Status of Research on Pollen Vitality and Stigma Receptivity of Wuren Walnut(Juglans sigillata)in Guizhou Province

Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste compared with ordinary walnut.The color of its inner seed coat is different from that of ordinary walnut,which is mostly light yellow.Wuren walnut has great market potential for development.To lay a theoretical foundation for the future breeding of improved varieties,pollination tree configuration,and hybrid breeding of Wuren walnut in Guizhou Province,this paper summarized the current research status of pollen vitality and stigma acceptability of Guizhou Wuren walnut in recent years,and looked forward to future research and application.

Earthworm rather than biochar and sodium silicate addition increased bacterial diversity in mining areas subjected to chemical fertilization

The addition of biochar,sodium silicate,or earthworm is a feasible practice to repair soils disturbed by mining activities,and the reclamation is largely based on the alteration of the diversity and structure of soil bacteria.The objectives of this study were to assess the relative importance of these supplements on soil bacterial community diversity and structure in reclaimed mine areas.A field experiment with soybean was carried out in mining areas to assess the efficiency of nitrogen,phospho-rus and potassium(NPK)fertilizers plus those supplements on soil bacterial community structure and diversity by the 16S rRNA sequencing method.Soil chemical properties were analyzed to their effects on the bacterial community structure.The results showed that the application of nitrogen,phosphorus and potassium(NPK)fertilizers significantly increased bacterial diversity,and a further increase was observed in NPK plus biochar,sodium silicate or earthworm addition.Furthermore,a higher number of genera were found in the NPK plus biochar and NPK plus earthworm treatments than that in the control,NPK and NPK plus sodium silicate treatments.The bacterial community was significantly associated with nutrients,such as carbon(C)and nitrogen(N).Moreover,soil organic carbon(SOC)and pH were the most dominant factors in shaping the soil bacterial community structure and diversity.Our data indicate that the addition of earthworms to soil rather than biochar and sodium silicate was the best strategy to mitigate the detrimental effects of mining activities on soil bacterial diversity.