The functional components and mechanism of Linderae Radix in treating diabetic nephropathy based on the network pharmacology

Objective:To analyze the components and mechanisms of Wuyao(Linderae Radix)in treating diabetic nephropathy(DN)based on network pharmacology.Methods:Multiple online databases were used to search and screen out the active ingredients from Linderae Radix,the related targets of active components of Linderae Radix and the genes related to DN.Search the corresponding genes name of target through UniProt database.Cytoscape 3.2.1 was used to construct the corresponding target gene network of Linderae Radix compounds.Venn diagram was used to screen the intersection genes of the active components corresponding to the target and disease-related genes,and the intersection genes were constructed into the protein interaction relationship network.Finally,DAVID database was used to do GO function enrichment analysis and KEGG signaling pathway enrichment analysis for the intersection genes,and the results of GO and KEGG were visualized.Results:1.A total of 7 potential active ingredients and 100 target proteins were screened.2.There are a total of 34 intersection genes between the potential active ingredient target in Linderae Radix in DN.3.The top 10 of the interaction correlation between intersection proteins include:AR and NCOA2,NCOA2 and NR3C1,NCOA2 and PPARG,etc.4.There were 16 entries of molecular function,9 entries of cell component,47 entries of biological process and 18 entries of signaling pathway(P<0.05).Conclusion:DN was treated by Linderae Radix from multi-component,multi-target and multi-link synergies.

Effects of Suoquan Yishen Formula on Mice with Diabetic Nephropathy (DN)

[Objectives]The purpose of this paper was to study the effects of Suoquan Yishen formula on mice with diabetic nephropathy(DN).[Methods]The mice were randomly divided into normal group,model group,irbesartan group,and high and low-dose Suoquan Yishen formula groups.The blood glucose level,BUN,Scr excretion and GSH activity and CAT activity were detected.[Results]The blood glucose levels of mice in the irbesartan group and high and low-dose Suoquan Yishen formula groups decreased.The BUN,PRO and Scr levels were higher and the GSH and CAT activity was lower in the model group compared with the normal group.After administration of Suoquan Yishen formula,the BUN,PRO and Scr levels of the DN mice decreased significantly,and the GSH and CAT activity increased significantly.[Conclusions]Suoquan Yishen formula can reduce blood glucose level,reduce PRO,BUN and Scr levels,and increase CAT and GSH activity in DN mice,thus protecting the ND mice.