Fresh-keeping Effects of Different Concentrations of 6-BA and B9 on Carnation Cut Flower

Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.

Comparative transcriptome and proteome analysis of lily clones inoculated with Fusarium oxysporum f. sp. lilii

Basal bulb rot is the major factor restricting the production of lily,caused by Fusarium oxysporum f.sp.lilii.To systematically analyze the transcriptomic and proteomic responses of lily clones to F.oxysporum,we constructed six RNA-seq libraries and four iTRAQ proteomic libraries using lily resistant and susceptible clones sampled at 0,24 and 48 h post-inoculation(hpi)with F.oxysporum respectively.137,715 unigenes were generated,of which 7,667 were differentially expressed.1,679 and 4,051 differentially expressed genes(DEGs)between resistant and susceptible clones were isolated from samples collected at 0 and 48 hpi.Four hundred and thirty three and 155 DEGs were identified in resistant clones sampled at 24 and 48 hpi separately while 550 and 799 DEGs were isolated in the susceptible clones sampled at 24 and 48 hpi respectively.The results of iTRAQ analysis revealed 7,482 proteins in resistant and susceptible clones.Data analysis of transcriptome and proteome indicated that 5,735 proteins corresponded to mRNAs.Three hundred and sixteen and 1,052 DEGs had corresponding DEPs.At 48 hpi,the resistant clones showed 155 DEGs and 108 corresponding DEPs,while the susceptible clones showed 799 DEGs and 316 corresponding DEPs.In general,these results enhance comprehension of the defense response of lily resistant clones to F.oxysporum infection and provide valuable sequence data for studying the resistance mechanism.

Effects of Illumination on Growth and Development and Quality of Cut Flowers of Eustoma grandiflorum (Raf.) Shinners

Lucetta,a cultivar of Eustoma grandiflorum( Raf.) Shinners,was subjected to treatments of different light intensities( 100%,85%,70% and 55% of natural light intensity) and different light durations( 10-h natural lighting,12-h natural lighting,12-h natural lighting+ 2-h LED lighting,12-h natural lighting + 4-h LED lighting),and then its plant height,crown width,stem thickness,stalk thickness,total flower number,flowering period and growth situation were analyzed. The results show that E. grandiflorum( Raf.) Shinners is sensitive to the changes in light intensity. Under 85% of natural light intensity( 40 000-50 000 lux),E. grandiflorum( Raf.) Shinners grew faster,bloomed earlier with more flowers,and was taller,thicker,more robust,higher in yield,larger in pedicel length,and better in cut flower quality,without rosette phenomenon. Long-term exposure to low-intensity light would led to slow growth,thin stalk,delayed flowering,low yield and reduced cut flower quality in E. grandiflorum( Raf.) Shinners. The illumination duration of more than 12 h could promote the differentiation of flower buds,increase the plant height,increase the length of pedicels of E. grandiflorum( Raf.) Shinners. Extending the light duration by 2-4 h at night,that is,14-16 h of illumination was the most ideal illumination time for the growth of E. grandiflorum( Raf.)Shinners. A longer photoperiod could promote the growth and bring forward the blooming of E. grandiflorum( Raf.) Shinners. The longer the illumination time,the higher the plant. The optimum illumination time was between 14 and 16 h. When the illumination duration was shorter than 12 h,the growth of E. grandiflorum( Raf.) Shinners was retarded,and its flowering was also delayed.