PURPOSE: To determine whether the location of a fluorescein-stained line, the Marx line (ML), which runs along the inner eyelid, is correlated with meibomian gland function. DESIGN: Prospective observational case seri...PURPOSE: To determine whether the location of a fluorescein-stained line, the Marx line (ML), which runs along the inner eyelid, is correlated with meibomian gland function. DESIGN: Prospective observational case series. METHODS: After applying fluorescein dye solution to the eye, the ML score was calculated for the outer, middle, and inner thirds of the lower eyelid margin. ML scoring was as follows: 0, entirely on the conjunctival side of the meibomian orifices (MOs); 1, part of the ML touches the MOs; 2, ML runs through all of the MOs; and 3, ML runs on the eyelid-margin side of the MOs. Correlations were calculated between the total ML score and age for 251 randomly recruited subjects without acute ocular surface diseases, and between age and the ML score for the three regions of the lower eyelid. Correlations between the regional ML score and the meibographic score, and the meibomian gland secretion score were also determined. The total ML scores of 15 subjects without meibomian gland dysfunction (MGD) were compared with 15 age-matched patients with MGD. RESULTS: The three regions of the lower eyelid had significantly different ML scores. Strong correlations were found between the ML score and age, the meibographic score, and the meibomian gland secretion score. The total ML score of MGD group was significantly higher than that of the non-MGD group. CONCLUSIONS: The strong correlation between the ML score and the meibomian gland scores indicates that the ML score can be used as a simple and rapid screening score for meibomian gland function.展开更多
Adult T-cell leukaemia/lymphoma is alymphopro life rativedis ord eraetiologi call yassociated with human T-cell lymphotropic virus type I infection. A cutan eous lesion often develops in the disease, and in rare cases...Adult T-cell leukaemia/lymphoma is alymphopro life rativedis ord eraetiologi call yassociated with human T-cell lymphotropic virus type I infection. A cutan eous lesion often develops in the disease, and in rare cases, is even the only m anifestation. Here we report a rare case of ‘cutaneous’adult T-cell leukaemia /lymphoma with neither atypical cells in the peripheral blood nor lymph node inv olvement. All nodular lesions were completely eliminated after local electron be am irradiation (20 Gy/nodule in total). To evaluate whether or not there were re sidual lymphoma cells in the skin, we performed PCR to detect clonal T cell rece ptor γgene rearrangements. The sample from the nodule before irradiation showed evidence of a rearranged band, which was not detected at the same site after tr eatment nor in any peripheral blood. The findings suggest that this procedure is useful for the evaluation of therapeutic effects and the early detection of lym phoma recurrence.展开更多
Background: Colonoscopic and clinical differences between primary ileocolonic mucosa-associated lymphoid tissue (MALT) lymphoma and mantle cell lymphoma (MCL) have not been defined. Methods: We reviewed colonoscopic a...Background: Colonoscopic and clinical differences between primary ileocolonic mucosa-associated lymphoid tissue (MALT) lymphoma and mantle cell lymphoma (MCL) have not been defined. Methods: We reviewed colonoscopic and clinical features in eight patients with primary MALT lymphoma and eight patients with MCL in the terminal ileum and/or colorectum. All cases were examined for CD5 and/or cyclin D1 expression. Results: Endoscopic features of MALT lymphoma were characterized as protrusions that were covered with normal-appearing mucosa with or without ulceration. The gross appearances of MALT lymphomas were categorized as solitary (4 patients), multiple (3 patients), and multiple lymphomatous polyposis (MLP) (1 patient). The gross features of MCL at endoscopy were categorized as multiple protrusions (2 patients), and MLP (6 patients). The clinical stages of patients with MCL were more advanced than in patients with MALT lymphoma. Conclusions: Solitary or multiple protrusions at an early clinical stage is the most common presentation pattern of patients with MALT lymphoma, but an MLP appearance at an early stage is also possible. On the other hand, MLP appearance with an advanced clinical stage is the main presentation pattern in patients with MCL, although multiple protrusions with an early clinical stage is also possible. Histological and immunohistochemical investigation including that of cyclin D1 and CD5 expression is essential to make the final diagnosis.展开更多
Objective.Abnormalities of the blood coagulation system have an influence on outcome in patients with fulminant hepatic failure(FHF).The protein C(PC)pathway is one of the main modulators of the blood coagulation syst...Objective.Abnormalities of the blood coagulation system have an influence on outcome in patients with fulminant hepatic failure(FHF).The protein C(PC)pathway is one of the main modulators of the blood coagulation system.The role of the PC pathway in FHF is not clear.In the present study,we evaluated endothelial cell injury and the grade of activated protein C(APC)generation in FHF patients.Material and methods.The effect of APC on the expression of tumor necrosis factor(TNF)-αand monocyte chemoattractant protein(MCP)-1 from LI90 stellate cells was also evaluated.This study comprised 5 patients with FHF,6 with acute hepatitis(AH),12 with chronic hepatitis(CH)and 20 healthy subjects.Results.The plasma concentrations of thrombin-antithrombin complex and thrombomodulin were significantly increased in FHF patients compared with those in AH patients and healthy subjects.The circulating levels of activated protein C-protein C inhibitor(APC-PCI)complex and the APC-PCI/PC ratio were significantly decreased in patients with FHF compared to healthy controls.APC significantly inhibited in vitro the expression of TNF-αand MCP-1 from LI90 stellate cells.Conclusions.This study demonstrated enhanced endothelial cell injury in association with decreased PC activation and hypercoagulability in FHF.展开更多
To determine whether mutations of the homeobox region of the human NOBOX gene are present in Japanese women with premature ovarian failure (POF), genomic DNA of blood samples from 30 women with POF and 20 normal contr...To determine whether mutations of the homeobox region of the human NOBOX gene are present in Japanese women with premature ovarian failure (POF), genomic DNA of blood samples from 30 women with POF and 20 normal controls were extracted for polymerase chain reaction (PCR) and analyzed by direct sequencing. No mutations in exons 2-6 of the NOBOX gene, including the homeobox region, could be identified in Japanese women with POF. Our data suggest that mutations of the homeobox region of the NOBOX gene are uncommon in Japanese patients with POF.展开更多
文摘PURPOSE: To determine whether the location of a fluorescein-stained line, the Marx line (ML), which runs along the inner eyelid, is correlated with meibomian gland function. DESIGN: Prospective observational case series. METHODS: After applying fluorescein dye solution to the eye, the ML score was calculated for the outer, middle, and inner thirds of the lower eyelid margin. ML scoring was as follows: 0, entirely on the conjunctival side of the meibomian orifices (MOs); 1, part of the ML touches the MOs; 2, ML runs through all of the MOs; and 3, ML runs on the eyelid-margin side of the MOs. Correlations were calculated between the total ML score and age for 251 randomly recruited subjects without acute ocular surface diseases, and between age and the ML score for the three regions of the lower eyelid. Correlations between the regional ML score and the meibographic score, and the meibomian gland secretion score were also determined. The total ML scores of 15 subjects without meibomian gland dysfunction (MGD) were compared with 15 age-matched patients with MGD. RESULTS: The three regions of the lower eyelid had significantly different ML scores. Strong correlations were found between the ML score and age, the meibographic score, and the meibomian gland secretion score. The total ML score of MGD group was significantly higher than that of the non-MGD group. CONCLUSIONS: The strong correlation between the ML score and the meibomian gland scores indicates that the ML score can be used as a simple and rapid screening score for meibomian gland function.
文摘Adult T-cell leukaemia/lymphoma is alymphopro life rativedis ord eraetiologi call yassociated with human T-cell lymphotropic virus type I infection. A cutan eous lesion often develops in the disease, and in rare cases, is even the only m anifestation. Here we report a rare case of ‘cutaneous’adult T-cell leukaemia /lymphoma with neither atypical cells in the peripheral blood nor lymph node inv olvement. All nodular lesions were completely eliminated after local electron be am irradiation (20 Gy/nodule in total). To evaluate whether or not there were re sidual lymphoma cells in the skin, we performed PCR to detect clonal T cell rece ptor γgene rearrangements. The sample from the nodule before irradiation showed evidence of a rearranged band, which was not detected at the same site after tr eatment nor in any peripheral blood. The findings suggest that this procedure is useful for the evaluation of therapeutic effects and the early detection of lym phoma recurrence.
文摘Background: Colonoscopic and clinical differences between primary ileocolonic mucosa-associated lymphoid tissue (MALT) lymphoma and mantle cell lymphoma (MCL) have not been defined. Methods: We reviewed colonoscopic and clinical features in eight patients with primary MALT lymphoma and eight patients with MCL in the terminal ileum and/or colorectum. All cases were examined for CD5 and/or cyclin D1 expression. Results: Endoscopic features of MALT lymphoma were characterized as protrusions that were covered with normal-appearing mucosa with or without ulceration. The gross appearances of MALT lymphomas were categorized as solitary (4 patients), multiple (3 patients), and multiple lymphomatous polyposis (MLP) (1 patient). The gross features of MCL at endoscopy were categorized as multiple protrusions (2 patients), and MLP (6 patients). The clinical stages of patients with MCL were more advanced than in patients with MALT lymphoma. Conclusions: Solitary or multiple protrusions at an early clinical stage is the most common presentation pattern of patients with MALT lymphoma, but an MLP appearance at an early stage is also possible. On the other hand, MLP appearance with an advanced clinical stage is the main presentation pattern in patients with MCL, although multiple protrusions with an early clinical stage is also possible. Histological and immunohistochemical investigation including that of cyclin D1 and CD5 expression is essential to make the final diagnosis.
文摘Objective.Abnormalities of the blood coagulation system have an influence on outcome in patients with fulminant hepatic failure(FHF).The protein C(PC)pathway is one of the main modulators of the blood coagulation system.The role of the PC pathway in FHF is not clear.In the present study,we evaluated endothelial cell injury and the grade of activated protein C(APC)generation in FHF patients.Material and methods.The effect of APC on the expression of tumor necrosis factor(TNF)-αand monocyte chemoattractant protein(MCP)-1 from LI90 stellate cells was also evaluated.This study comprised 5 patients with FHF,6 with acute hepatitis(AH),12 with chronic hepatitis(CH)and 20 healthy subjects.Results.The plasma concentrations of thrombin-antithrombin complex and thrombomodulin were significantly increased in FHF patients compared with those in AH patients and healthy subjects.The circulating levels of activated protein C-protein C inhibitor(APC-PCI)complex and the APC-PCI/PC ratio were significantly decreased in patients with FHF compared to healthy controls.APC significantly inhibited in vitro the expression of TNF-αand MCP-1 from LI90 stellate cells.Conclusions.This study demonstrated enhanced endothelial cell injury in association with decreased PC activation and hypercoagulability in FHF.
文摘To determine whether mutations of the homeobox region of the human NOBOX gene are present in Japanese women with premature ovarian failure (POF), genomic DNA of blood samples from 30 women with POF and 20 normal controls were extracted for polymerase chain reaction (PCR) and analyzed by direct sequencing. No mutations in exons 2-6 of the NOBOX gene, including the homeobox region, could be identified in Japanese women with POF. Our data suggest that mutations of the homeobox region of the NOBOX gene are uncommon in Japanese patients with POF.
